Polymer and lipid-based nanoparticles to deliver RNAi and CRISPR systems

Chandrasekaran, Rajkuberan; Seetharaman, Prabu Kumar; Danaraj, Jeyapragash; Periakaruppan, Rajiv; Abd‐Elsalam, Kamel A.

doi:10.1016/b978-0-12-821910-2.00016-3

Cited by 1 publication

(1 citation statement)

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“…This makes these systems similar to real-life systems [ 97 ]. The notable benefit of cationic lipid nanoparticles over polymeric NPs is that they can be generated without the use of organic solvents using a high-pressure homogenization (HPH) process [ 98 ]. Cationic lipid nanoparticles with at least one cationic lipid have been described as non-viral vectors for delivery of genes [ 99 ].…”

Section: Nanoparticle-based Carriers In Crispr/casmentioning

confidence: 99%

Exosome/Liposome-like Nanoparticles: New Carriers for CRISPR Genome Editing in Plants

Alghuthaymi

Ahmad

Khan

et al. 2021

IJMS

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Rapid developments in the field of plant genome editing using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems necessitate more detailed consideration of the delivery of the CRISPR system into plants. Successful and safe editing of plant genomes is partly based on efficient delivery of the CRISPR system. Along with the use of plasmids and viral vectors as cargo material for genome editing, non-viral vectors have also been considered for delivery purposes. These non-viral vectors can be made of a variety of materials, including inorganic nanoparticles, carbon nanotubes, liposomes, and protein- and peptide-based nanoparticles, as well as nanoscale polymeric materials. They have a decreased immune response, an advantage over viral vectors, and offer additional flexibility in their design, allowing them to be functionalized and targeted to specific sites in a biological system with low cytotoxicity. This review is dedicated to describing the delivery methods of CRISPR system into plants with emphasis on the use of non-viral vectors.

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Section: Nanoparticle-based Carriers In Crispr/casmentioning

confidence: 99%