Exosome/Liposome-like Nanoparticles: New Carriers for CRISPR Genome Editing in Plants

Alghuthaymi, Mousa A.; Ahmad, Aftab; Khan, Zulqurnain; Khan, Sultan Habibullah; Ahmed, Farah K.; Faiz, Sajid; Nepovimová, Eugenie; Kuča, Kamil; Abd-Elsalam, Kamel A.

doi:10.3390/ijms22147456

Cited by 44 publications

(21 citation statements)

References 150 publications

(125 reference statements)

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“…EVs can be utilized as efficient transport carriers with the advantages of design flexibility, functionalized modification, targeted delivery, and low cytotoxicity in an integrated systematic mode ( Alghuthaymi et al, 2021 ). Li et al (2020) reported a nanoplatform comprising engineered macrophage exosomes, c-Met-targeting peptide, and DOX, which exhibited an excellent antitumor effect in TNBC xenograft.…”

Section: Extracellular Vesicles In Triple-negative Breast Cancer Trea...mentioning

confidence: 99%

Extracellular Vesicles: The Landscape in the Progression, Diagnosis, and Treatment of Triple-Negative Breast Cancer

Dong

Liu

et al. 2022

Front. Cell Dev. Biol.

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Triple-negative breast cancer (TNBC) is a heterogeneous subtype of breast cancer (BC) with diverse biological behavior, high aggressiveness, and poor prognosis. Extracellular vesicles (EVs) are nano-sized membrane-bound vesicles secreted by nearly all cells, and are involved in physiological and pathological processes. EVs deliver multiple functional cargos into the extracellular space, including proteins, lipids, mRNAs, non-coding RNAs (ncRNAs), and DNA fragments. Emerging evidence confirms that EVs enable pro-oncogenic secretome delivering and trafficking for long-distance cell-to-cell communication in shaping the tumor microenvironment (TME). The transferred tumor-derived EVs modify the capability of invasive behavior and organ-specific metastasis in recipient cells. In addition, TNBC cell-derived EVs have been extensively investigated due to their promising potential as valuable biomarkers for diagnosis, monitoring, and treatment evaluation. Here, the present review will discuss the recent progress of EVs in TNBC growth, metastasis, immune regulation, as well as the potential in TNBC diagnosis and treatment application, hoping to decipher the advantages and challenges of EVs for combating TNBC.

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Section: Extracellular Vesicles In Triple-negative Breast Cancer Trea...mentioning

confidence: 99%

Extracellular Vesicles: The Landscape in the Progression, Diagnosis, and Treatment of Triple-Negative Breast Cancer

Dong

Liu

et al. 2022

Front. Cell Dev. Biol.

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“…In food science, they are used for the protection and controlled delivery of enzymes, vitamins, functional compounds, antibiotics, and metabolites to improve the properties of food [62]. In plants, in addition to the reports in Table 2 for RNAi delivery, they are used as carriers of CRISPR/Cas gene-editing systems, proteins, DNA, or mRNA [63]. Additionally, lipofection (liposome-mediated DNA delivery) in protoplasts [64] and nutrient transport/internalization are cited [65].…”

Section: Liposomesmentioning

confidence: 99%

RNAi Crop Protection Advances

Hernández-Soto

Chacón-Cerdas

2021

IJMS

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RNAi technology is a versatile, effective, safe, and eco-friendly alternative for crop protection. There is plenty of evidence of its use through host-induced gene silencing (HIGS) and emerging evidence that spray-induced gene silencing (SIGS) techniques can work as well to control viruses, bacteria, fungi, insects, and nematodes. For SIGS, its most significant challenge is achieving stability and avoiding premature degradation of RNAi in the environment or during its absorption by the target organism. One alternative is encapsulation in liposomes, virus-like particles, polyplex nanoparticles, and bioclay, which can be obtained through the recombinant production of RNAi in vectors, transgenesis, and micro/nanoencapsulation. The materials must be safe, biodegradable, and stable in multiple chemical environments, favoring the controlled release of RNAi. Most of the current research on encapsulated RNAi focuses primarily on oral delivery to control insects by silencing essential genes. The regulation of RNAi technology focuses on risk assessment using different approaches; however, this technology has positive economic, environmental, and human health implications for its use in agriculture. The emergence of alternatives combining RNAi gene silencing with the induction of resistance in crops by elicitation and metabolic control is expected, as well as multiple silencing and biotechnological optimization of its large-scale production.

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“…In food science, they are used for the protection and controlled delivery of enzymes, vitamins, functional compounds, antibiotics, and metabolites to improve the properties of food 54 . In plants, in addition to the reports in Table 2 for RNAi delivery, they are used as carriers of CRISPR/Cas gene-editing systems, proteins, DNA, or mRNA 55 . Also, lipofection (liposome-mediated DNA delivery) in protoplasts 56 , and nutrient transport /internalization are cited 57 .…”

Section: Liposomesmentioning

confidence: 99%

RNAi Crop Protection Advances

Hernández-Soto¹,

Chacón-Cerdas²

2021

Preprint

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RNAi technology is a versatile, effective, safe, and eco-friendly alternative for crop protection. There is plenty of evidence of its use through Host-induced gene silencing (HIGS) and spray-induced gene silencing (SIGS) techniques to control viruses, bacteria, fungi, insects, and nematodes. As for SIGS, its most significant challenge is achieving stability and avoiding premature degradation of RNAi in the environment or during its absorption in the target organism. One alternative is the encapsulation in liposomes, virus-like particles, polyplex nanoparticles, and bio-clay, which can be obtained through the recombinant production of RNAi in vectors, transgenesis, and micro/nanoencapsulation. The materials must be safe, biodegradable, and stable in multiple chemical environments favoring the controlled release of RNAi. Most of the current research of encapsulated RNAi focuses primarily on oral delivery to control insects by silencing essential genes. The regulation of RNAi technology focuses on risk assessment from different approaches; however, this technology has positive characteristics for its use in agriculture from the economic, environmental, and human health implications. The emergence of alternatives combining RNAi gene silencing with the induction of resistance in crops by elicitation and metabolic control is expected, as well as multiple silencing and biotechnological optimization of its large-scale production.

show abstract

Exosome/Liposome-like Nanoparticles: New Carriers for CRISPR Genome Editing in Plants

Cited by 44 publications

References 150 publications

Extracellular Vesicles: The Landscape in the Progression, Diagnosis, and Treatment of Triple-Negative Breast Cancer

Extracellular Vesicles: The Landscape in the Progression, Diagnosis, and Treatment of Triple-Negative Breast Cancer

RNAi Crop Protection Advances

RNAi Crop Protection Advances

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