This paper describes a simple, convenient approach to the fabrication of microband electrodes and microband biosensors based on screen printing technology. These devices were printed in a three‐electrode configuration on one strip; a silver/silver chloride electrode and carbon counter electrode served as reference and counter electrodes respectively. The working electrodes were fabricated by screen‐printing a water‐based carbon ink containing cobalt phthalocyanine for hydrogen peroxide detection. These were converted into a glucose microband biosensor by the addition of glucose oxidase into the carbon ink. In this paper, we discuss the fabrication and application of glucose microband electrodes for the determination of glucose in cell media. The dimensions (100–400 microns) of the microband electrodes result in radial diffusion, which results in steady state responses in the absence of stirring. The microband biosensors were investigated in cell media containing different concentrations of glucose using chronoamperometry. The device shows linearity for glucose determination in the range 0.5 mM to 2.5 mM in cell media. The screen‐printed microband biosensor design holds promise as a generic platform for future applications in cell toxicity studies.
10This paper describes the development of a simple, low cost chronoamperometric assay, for the measurement of 11 fructose, using a graphite-nanoparticle modified screen-printed electrode (SPCE-G-COOH). Cyclic voltammetry
12showed that the response of the SPCE-G-COOH enhanced the sensitivity and precision, towards the enzymatically 13 generated ferrocyanide species, over a plain SPCE; therefore the former was employed in subsequent studies.14 Calibration studies were carried out using chronoamperometry with a 40 µl mixture containing fructose, mediator 15 and FDH, deposited onto the SPCE-G-COOH. The response was linear from 0.1 mM to 1.0 mM. A commercial 16 fruit juice sample was analysed using the developed assay and the fructose concentration was calculated to be 477 17 mM with a precision of 3.03 % (n=5). Following fortification (477 mM fructose) the mean recovery was found to be 18 97.12 % with a coefficient of variation of 6.42 % (n=5); consequently, the method holds promise for the analysis of
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