Development of a simple, low cost chronoamperometric assay for fructose based on a commercial graphite-nanoparticle modified screen-printed carbon electrode

Abstract

10This paper describes the development of a simple, low cost chronoamperometric assay, for the measurement of 11 fructose, using a graphite-nanoparticle modified screen-printed electrode (SPCE-G-COOH). Cyclic voltammetry 12showed that the response of the SPCE-G-COOH enhanced the sensitivity and precision, towards the enzymatically 13 generated ferrocyanide species, over a plain SPCE; therefore the former was employed in subsequent studies.14 Calibration studies were carried out using chronoamperometry with a 40 µl mixture containing fructose, mediator 15 and FDH, deposited onto the SPCE-G-COOH. The response was linear from 0.1 mM to 1.0 mM. A commercial 16 fruit juice sample was analysed using the developed assay and the fructose concentration was calculated to be 477 17 mM with a precision of 3.03 % (n=5). Following fortification (477 mM fructose) the mean recovery was found to be 18 97.12 % with a coefficient of variation of 6.42 % (n=5); consequently, the method holds promise for the analysis of

A review of screen-printed silver/silver chloride (Ag/AgCl) reference electrodes potentially suitable for environmental potentiometric sensors

“…After the appearance of novel conducting polymers, ionic liquids and other carbon-based materials, the scientific community has tried to implement them for the development of a reliable screen-printed reference electrode not necessarily of the Ag/AgCl type. Although in the past, Ag/AgCl reference electrode seemed to be the most promising type of screen-printed reference electrodes, nowadays most scientists have moved to other directions such as ionophore-doped membranes using ionic liquids, nanomaterials [57], solid ion-conducting materials such as Nafion™ and more commonly, conducting polymers. Conducting polymers feature several advantages since they are in the solid form, can conduct electricity with very low currents and have low impedances, they are low-cost and they can be used as screen-printing inks.…”

“…Salt matrix A wide variety of voltammetric, amperometric and potentiometric sensors have been reported that implement QREs while they have also been used in cyclic voltammetry. The construction and fabrication of Ag/AgCl QREs has been achieved by different approaches and on several different types of substrates, each one serving the specific application [49], [57]- [61]. Choi et al in 2001 [62], developed a screen-printed disposable sensor for the anodic stripping voltammetric (ASV) analysis of heavy metals.…”

Recent Updates on Electrochemical Detection of Perchlorate Ions

“…We were taken screen printed electrode and modifying them using different materials. In past few years many nano-materials was used for modifying electrodes [32], [47], [70]. After modifying samples are performed on that electrode using following equation: X 1 + HClO 4 X 2 + Cl -Here we mixed X1 solvent into HClO 4 solution at different terms and conditions and performed electrochemical experiment.…”

Direct electrochemical detection of clozapine by RuO2 nanoparticles-modified screen-printed electrode

“…Furthermore, these electrodes are appropriate for operations with micro volumes and decentralized assays (point of care tests), and so forth. [19][20][21][22][23] To minimize the oxidation over-potential of numerous electroactive compounds, researchers designed the electrocatalytic procedures for exploitation at the modied electrode surface. Notably, the chemically modied electrodes augment the transfer rate of electrons by declining the over-potential.…”