A combined sensor strip was fabricated by screen-printing a Meldola Blue modified carbon working electrode and a Ag/AgCl combined reference/counter electrode alongside each other on a PVC substrate. This was investigated as an amperometric sensor for NADH, at an applied potential of OV, both in bulk solutions under hydrodynamic conditions and using 20 ~L L volumes without agitation. The former technique gave linear responses in the range 3-60 x mot dm-) of NADH. Further modification of the electrodes with NAD+ and lactate dehydrogenase converted the device to a biosensor for lactate both in spiked phosphate buffer and bovine serum. The sensors demonstrated good linear responses in the range 0-20 x Keywords: NADH, Lactate, Chronoamperometry, Screen-printing, Biosensor mol dm-3 and the latter in the range 10-5 x moldm-).
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