Fabrication of Miniaturised Screen‐printed Glucose Biosensors, Using a Water‐based Ink, and the Evaluation of their Electrochemical Behaviour

Abstract

This paper describes a simple, convenient approach to the fabrication of microband electrodes and microband biosensors based on screen printing technology. These devices were printed in a three‐electrode configuration on one strip; a silver/silver chloride electrode and carbon counter electrode served as reference and counter electrodes respectively. The working electrodes were fabricated by screen‐printing a water‐based carbon ink containing cobalt phthalocyanine for hydrogen peroxide detection. These were converted into a glucose microband biosensor by the addition of glucose oxidase into the carbon ink. In this paper, we discuss the fabrication and application of glucose microband electrodes for the determination of glucose in cell media. The dimensions (100–400 microns) of the microband electrodes result in radial diffusion, which results in steady state responses in the absence of stirring. The microband biosensors were investigated in cell media containing different concentrations of glucose using chronoamperometry. The device shows linearity for glucose determination in the range 0.5 mM to 2.5 mM in cell media. The screen‐printed microband biosensor design holds promise as a generic platform for future applications in cell toxicity studies.

Impact of nano-morphology, lattice defects and conductivity on the performance of graphene based electrochemical biosensors

“…Nowadays, amperometric biosensors are commercially available and widely used for glucose monitoring, reflecting the pioneering design’s effectiveness. Exemplifying current design progress, a miniaturised screen printed biosensor (100–400 microns), using a water-based ink containing cobalt phthalocyanine, detected glucose by chronoamperometry (CA) in the linear range from 0.5 to 2.5 mM [14], appropriate for cell toxicity applications.…”

Chitosan-based enzyme ink for screen-printed bioanodes

“…16 Possibly because of this need for a complicated optimization, as well as for in-house printing facilities in the developing laboratory, the number of reported bio-composite inks for one-step deposition for fabrication of biosensors and BFCs is limited. [16][17][18][19][20][21][22] Furthermore, to the best of our knowledge, all printable bio-composite inks have been fabricated using graphite as a conductive material.…”

Reusable, Non-Invasive, and Ultrafast Radio Frequency Biosensor Based on Optimized Integrated Passive Device Fabrication Process for Quantitative Detection of Glucose Levels

“…With the aid of chips, microfluidics systems and labs-on-a-chip, biosensor technology have developed rapidly and can be divided into electrochemical biosensors and optical biosensors based on the type of signal conversion [17][18][19][20][21][22][23][24][25][26]. Electrochemical biosensors enable the detection of biomarkers by adding specific enzymes on the electrodes, which has many advantages, such as high sensitivity, simple operation and cost-effective [27][28][29]. However, the introduction of some small molecule exotic vectors acting as channels between enzymes and motors electrodes leads to a slowing reaction, a decreasing performance, a deteriorating reliability and frequent replacement.…”