Introduction the management of anal fistula remains debatable. The lack of a standard treatment free of complications stimulates the development of new options. Objective to develop an experimental model of anal fistula in rats. Methods to surgically create an anal fistula in 10 rats with Seton introduced through the anal sphincter musculature.The animals were euthanized for histological fistula tract assessment. Results all ten specimens histologically assessed had a lumen and surrounding granulation tissue. There was complete epithelialization of the tract in two samples, halfway epithelialization in one sample and epithelialization of only the outer portion in six samples. Epithelialization was not evident in one tract. Conclusion anal fistulas in rats were histologically proved.

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To analyze the therapeutic potentials of different hydroxyapatites used for the correction of bone defects in rats. Methods: Forty rats, male, albino wistar, were distributed in 4 groups. They were submitted to a 3.5 mm defect in tibia. They received low purity hydroxyapatite, Strontium hydroxyapatite and hydroxyapatite doped with gallium, having a seven day evaluation time. Histopathology slides were stained with hematoxylin-eosin, for morphological evaluation. Were analyzed inflammatory processes, necrosis, presence of osteoclasts and osteoblasts, presence of the material, presence of white cells, neovascularization and bone neoformation. Results: It was observed that the groups HAPSr and HAPGa, presented better results of trabecular bone, hyaline cartilage and bone marrow more organized. Conclusion: There was improvement in the repair of the bone defect produced, showing that these hydroxyapatites are effective osteoinductive, osteoconductive, osteintegrant agents and have biocompatibility, and may be indicated for use in defect repairs.

Ischemic postconditioning was able to minimize reperfusion injury of rats undergone mesenteric ischemia and reperfusion process. There was no difference in the effectiveness of the method comparing two cycles of two minutes with four cycles of 30 seconds by H&E histological evaluation of the ileum after 60-minute reperfusion.

IntroductionIschemic postconditioning has been recognized as effective in the prevention of reperfusion injury in situations of ischemia and reperfusion in various organs and tissues. However, it remains unclear what would be the best way to accomplish it, since studies show great variation in the method of their application.ObjectiveTo assess the protective effect of ischemic postconditioning on ischemia and reperfusion in rats undergoing five alternating cycles of reperfusion and ischemia of 30 seconds each one.MethodsWe studied 25 Wistar rats distributed in three groups: group A (10 rats), which underwent mesenteric ischemia (30 minutes) and reperfusion (60 minutes); Group B (10 rats), undergoing ischemia (30 minutes) and reperfusion (60 minutes), intercalated by postconditioning (5 alternating cycles of reperfusion and ischemia of 30 seconds each one); and group C - SHAM (5 rats), undergoing only laparotomy and manipulation of mesenteric artery. All animals underwent resection of an ileum segment for histological analysis.ResultsThe mean lesions degree according to Chiu et al. were: group A, 2.77, group B, 2.67 and group C, 0.12. There was no difference between groups A and B (P>0.05).ConclusionIschemic postconditioning was not able to minimize or prevent the intestinal tissue injury in rats undergoing ischemia and reperfusion process when used five cycles lasting 30 seconds each one.

PURPOSE: To demonstrate the irreversible poisoning action of the acetone cyanohydrin (AC) in malignant cells. METHODS:Thirty male Swiss mice were inoculated with 1x10³ Ehrlich tumor (ET) cells. The mice were divided into three groups (n=10): CG (saline); ACG1 (1.864 mg/Kg of AC) and ACG2 (2.796 mg/Kg of AC), treated every 48 hours from day 3 until day 13. On day 15 the mice were euthanized and the number of viable cells in ascites was determined. In the meantime, ET cells were incubated with AC (0.5, 1.0, 2.0 μg/mL). Cell viability and percentage of growth inhibition (PGI) were checked after one, two, three, four, 18 and 24 hours. RESULTS:There was reduction in volume and number of viable cells in ACG1 and ACG2 compared to CG. In ACG1 one of the animals did not present ascites. In ACG2 two mice did not present ascites and in CG none of the mice present ascites. The action of AC was dose and time dependent and there was no significant difference among the three doses. CONCLUSION:The acetone cyanohydrin promoted reduction of the tumor and also prevented tumor development in 20% of the treated animals.

INTRODUCTION Some publications have demonstrated the presence of lung reperfusion injury in mesenteric ischemia and reperfusion (I/R), but under to diverse methods. Postconditioning has been recognized as effective in preventing reperfusion injury in various organs and tissues. However, its effectiveness has not been evaluated in the prevention of lung reperfusion injury after mesenteric ischemia and reperfusion.OBJECTIVE To evaluate the presence of pulmonary reperfusion injury and the protective effect of ischemic postconditioning on lung parenchyma in rats submitted to mesenteric ischemia and reperfusion.METHODS Thirty Wistar rats were distributed into three groups: group A (10 rats), which was held mesenteric ischemia (30 minutes) and reperfusion (60 minutes); group B (10 rats), ischemia and reperfusion, interspersed by postconditioning with two alternating cycles of reperfusion and reocclusion, for two minutes each; and group C (10 rats), ischemia and reperfusion interleaved by postconditioning with four alternating cycles of reperfusion and reocclusion of 30 seconds each. Finally, it was resected the upper lung lobe for histological analysis.RESULTS There were mild lung lesions (grade 1) in all samples. There was no statistical difference between groups 1 and 2 (P>0.05).CONCLUSION The mesenteric ischemia and reperfusion in rats for thirty and sixty minutes, respectively, caused mild reperfusion injury in lung. Postconditioning was not able to minimize the remote reperfusion injury and there was no difference comparing two cycles of two minutes with four cycles of 30 seconds.

Nós investigamos as soluções de uma equação hidrodinâmica de campo médio unidimensional utilizando aproximações variacionais. Modelamos analiticamente e comparamos dois condensados de Bose-Einstein que podem ser usados para criar gaps sólitons iluminados experimentalmente, um deles aprisionado por uma rede óptica duplamente periódica e o outro aprisionado por uma rede óptica simples. Nesses dois casos não utilizamos um confinamento harmônico adicional. Através da aproximação variacional nós estudamos a possibilidade de que o coeficiente de não linearidade atuando em uma combinação com o potencial da rede óptica duplamente periódica, ou com o potencial de uma rede óptica simples, permite o surgimento de gaps sólitons iluminados em uma dimensão. Em ambos os casos, nós analisamos a existência e estabilidade de gaps sólitons iluminados usando um ansatz gaussiano. Este artigo pode ser utilizado como um guia de aprendizagem no estudo de átomos frios; incentivando os alunos a realizarem cálculos variacionais para outros tipos de redes ópticas.