Cardiac autonomic dysfunction expressed by reduced baroreflex bradycardia was detected in rats chronically infected with T cruzi, as in human Chagas' disease. The disturbance, shown for the first time in an animal model of chagasic infection, resulted primarily from impaired efferent parasympathetic activity caused by intrinsic neuroganglionar lesions.
Ten male Wistar rats, chronically infected with Colombian, São Felipe (12SF) and Y strains of Trypanosoma cruzi and ten non-infected control animals were submitted to the bradycardia responsiveness test, an assessment of heart parasympathetic function, after phenylephrine injection. Six chagasic animals showed heart parasympathetic dysfunction characterized by reduction in the index of bradycardia baroreflex responsiveness, as compared with the control group. Microscopic examination of the atrial heart ganglia of chagasic rats showed ganglionitis, but no statistically significant reduction in the number of neurons.
The Griess assay is widely used by
regulation agencies as an official
method for nitrite quantification in water and food samples. In Brazil,
the official method, which has been used to determine nitrite in food,
was described by Instituto Adolfo Lutz (283/IV) in 1984. It uses 8
mL of reactants and provides 50 mL (reactants plus sample) of waste
per sample analyzed. Here, students scaled down the official method
50 times and quantified nitrite in water and food samples by using
just 0.2 mL of the Griess reactant and 1 mL of sample. Quantitative
analysis was carried out using absorbance measured at 540 nm (standard
method) and 96-well-plate images (proposed method) obtained with a
desktop scanner. The nitrite was extracted from solid food samples
by heating it in a water bath. After heating, sample color and turbidity
were eliminated by addition of K4[Fe(CN)6] and
ZnSO4 solutions and filtering. During samples preparation,
students evaluate the heating time effect in nitrite extraction from
sausage samples using hypothesis tests. Students did a series of matrix
matched samples to observe the matrix effect. Students also calculated
the detection limit (DL) and the quantification limit (QL) for the
proposed method (1.35 and 4.1 μmol/L nitrite, respectively)
and for the standard method (1.1 and 3.4 μmol/L nitrite, respectively).
DL and QL were determined using the standard deviation of the lowest
concentration point on the standard curve. The major pedagogical value
of this laboratory class was to scale down the official method and
use it to prepare and analyze a solid food sample. As a learning model,
finding real water samples, which have nitrite concentrations larger
than method QL, was a hard task, but all sausage samples analyzed
had larger nitrate concentrations than the method QL and nitrite quantification
in sausages was a good learning model.
In the present work, the ester synthesis in organic media catalyzed by lipases immobilized on chrysotile was studied. Lipases of different sources (Mucor javanicus, Pseudomonas cepacia, Rhizopus oryzae, Aspergillus niger and Candida rugosa) were immobilized on chrysotile, an inexpensive magnesium silicate, and used for esterification of hexanoic, octanoic and lauric acid with methanol, ethanol, 1-butanol and 1-octanol at 25 • C in hexane as solvent. The best results were obtained with Mucor javanicus lipase and lauric acid giving yields of 62-97% of ester.
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