One hundred seventy-six canine lymphomas were classified morphologically using four of the major human lymphoma classification schemes (Rappaport, Lukes-Collins, Kiel, and the Working Formulation). All 176 dogs received the same chemotherapeutic protocol. Sixty-two of these lymphomas had their immunophenotypes established by examination of cell surface markers by automated cytofluorography. Several different morphologic types of canine lymphoma were identified and these were comparable to morphologic categories in human classification schemes. Follicular and low grade lymphomas were rare. The two most common morphologic types were diffuse large cell (centroblastic) and immunoblastic. The Kiel classification appeared to be the most useful human scheme for classifying the canine lymphomas. Cytofluorographic analysis was generally straightforward, and 60 of the 62 lymphomas were placed into one of three immunophenotypic categories: 27 pan-T(LQ1)+SIg+, 21 pan-T(LQ1)-SIg+, and 12 pan-T(LQ1)+SIg-. Two of the lymphomas could not be characterized immunologically because a pre-existing or reactive non-neoplastic population of lymphocytes made interpretation of single cell suspension analysis difficult. The authors identified correlations between morphology and survival and disease-free remission; dogs with high-grade tumors generally survived the longest and had the longest remissions. No correlations were identified between high concentrations of serum lactate dehydrogenase, age, sex, or stage of disease, and morphology, immunophenotype, remission, or survival times. A significant correlation between clinical illness and survival time was documented. The median age of the dogs was nine years, no significant effect of sex on prevalence was observed, and some breeds were significantly overrepresented. Significant morphologic-immunophenotypic correlations included shorter remission and survival times for T-cell tumors than B-cell tumors, and a highly significant correlation between the pan-T(LQ1)+SIg-"T cell" phenotype and hypercalcemia.

Abstract. In a retrospective study of 71 primary ovarian tumors in the dog, epithelial tumors (46%) were more common than sex cord stromal (34%) and germ cell tumors (20%). There were more adenocarcinomas (64%) than adenomas. Sex cord stromal tumors were equally divided into Sertoli-Leydig (1 2/24) and granulosa cell tumors (12/24). There were equal numbers (7/14) of dysgerminomas and teratomas among the germ cell tumors. Most teratomas (6/7) were malignant. Most granulosa cell tumors were solid; two were mostly cystic. Patterns included sheets of round and ovoid to spindle-shaped cells separated by thin, fibrovascular stroma; neoplastic cells formed rosettes or Call-Exner bodies. In some areas, neoplastic cells were in cords or columns and formed cyst-like structures. Four granulosa cell tumors were macrofollicular, having cysts lined with granulosa cells. Median ages of dogs with different ovarian neoplasms were similar; all were more than 10 years old, except the dogs with teratoma (mean age, 4 years). Most neoplasms were unilateral (84'?0), except the SertoliLeydig cell tumors, many of which were bilateral (36%). Size of ovarian neoplasms varied (2 cm3 to 15,000 cm3). Twenty-nine percent of neoplasms metastasized; adenocarcinomas (48%) and malignant teratomas (50%) had the highest rates, and distant metastasis was more common in malignant teratoma. Endometrial hyperplasia was in 67% of the dogs; it was most common in dogs with sex cord stromal tumors (95%). Uterine malignancy was not seen in dogs with granulosa cell tumors, although hyperplastic endometrium was in all dogs with this tumor. Cysts in the contralateral ovaries were most common in dogs with sex cord stromal tumors.Canine ovarian neoplasms can be broadly divided into three major categories: (1) germ cell, (2) epithelial, and (3) sex cord stromal. The rate of occurrence of these three histologic types varies in different studies. Epithelial neoplasms are most common in some studies,2J2 and sex cord stromal tumors are most common in o t h e r~.~J~ Adenocarcinomas were more common than their benign counterparts in two studies that used the same source m a t e~i a l .~, '~ Germ cell tumors were less prevalent than the other two types of tumor in all these studies, and dysgerminomas were more common than teratomas in most of them.2s5*7J2J3 In all of these ~t u d i e s~,~,~J~ except one,I2 no distinction was made be- Materials and MethodsA 10-year retrospective survey of the medical records at The Animal Medical Center yielded 7 1 dogs with adequate tissue specimens to confirm a diagnosis of primary ovarian neoplasm and to classify the tumors. Contralateral ovarian and uterine specimens, whenever available, were also examined. Age, breed, clinical, and gross pathologic descriptions were obtained from records. Tissues were fixed in 10% buffered formalin, processed routinely, sliced at 6 pm, and stained with hematoxylin and eosin (HE), mucicarmine, periodic acid-Schiff (PAS), and modified Grimelius stains. Histologic classification was based on the majo...

Abstract. Fourteen ovarian germ cell tumors were diagnosed in the dog-seven dysgerminomas and seven teratomas. Six of the teratomas had immature components and were judged malignant; three had metastasized. One dysgerminoma had metastasized. The dysgerminomas developed in dogs ten years of age and older, and the teratomas developed in dogs nine years old and younger.

No abstract

No abstract

Trichrome-stained bronchial washings were obtained from 22 cats at necropsy and compared to lung tissue sections obtained at the same time. In three of the 22 cats, antemortem bronchial washings also were obtained. Sixteen of the cats were clinically ill and six were clinically normal and served as controls. On the basis of comparison of histologic sections and bronchial washings, patterns were detected in the washings indicative of general processes such as alveolar edema, chronic passive congestion, mucous metaplasia, and inflammation. Two specific etiologic agents, Histoplasma capsulatum and Toxoplasma gondii, each were seen in one washing.

No abstract

A prospective, clinical trial was conducted using 12 dogs that were presented for intestinal biopsy. Comparisons were made between paired jejunal biopsies collected using a Keyes biopsy punch and a standard scissor excisional technique. There were no differences in speed of collection, diagnostic value of specimens, complication rates, or sample quality. In this study, the Keyes skin punch was found to be safe, rapid, and diagnostic in all animals. Based on results of this study, the use of a Keyes punch instrument can be recommended as an alternative to other open intestinal biopsy methods.