The Effect of Aqueous Peat Extract on the Spontaneous Contractile Activity of Smooth MusclesBackground:There are reports in the literature about the effects of peat and its products on spontaneous contractile activity (SCA) of smooth muscles. In our earlier work we showed that the aqueous peat extract is a stimulus for SCA of smooth muscle fibres. After longstanding (4–5 h) influence from aqueous peat extract, a selfblocking effect of these influences can be observed. The mechanism of action could not be determined up to now. Purpose:The purpose of this study is to elucidate the peat mechanism of action. Material and Methods:In organ baths, the effects of aqueous peat extract on the SCA from smooth muscles have been studied under normal conditions and with presence of different receptor blocking substances. Results:Our investigations show that only with simultaneous blocking of α2 and D2 receptors the stimulating activity of aqueous peat extract on the SCA of smooth muscle fibres can be suppressed. The selfblocking actions of the stimulating activity of aqueous peat extract on the SCA of smooth muscles are interconnected with blocking of α2 adreno and D2 receptors. Conclusion:The aqueous peat extract contains biologically active substances, which have a partial agonistic effect on α2 and D2 dopamine receptors. Corresponding to the results we start out at pharmacological effects as they are known from the mentioned receptors. The clinical efficacy have to be shown by clinical trials.
Zusammenfassung Hintergrund: Heiltorf und Heilschlämme werden bei verschie− denen Indikationen in der Klinik erfolgreich angewandet. Unab− hängig von physikalisch−thermischen Wirkungen konnten neue Studien so genannte ¹chemische Wirkungen", bedingt durch die enthaltenen Torf− bzw. Schlamminhaltsstoffe, nachweisen. Diese zeichnen sich durch anregende Wirkungen auf die spontane kon− traktile Aktivität an der glatten Muskulatur aus. Das Ziel der vor− liegenden Arbeit ist es, herauszufinden, inwieweit wässeriger Schlammextrakt eine biologische Aktivität besitzt und inwieweit eine Vergleichbarkeit mit wässerigem Torfextrakt diesbezüglich besteht. Methoden: Die Untersuchungen wurden mit Heil− schlamm aus Pomorie (Bulgarien), wie er aus den dortigen Seen gewonnen wird, durchgeführt. Der fein strukturierte Heil− schlamm hat eine schwarze Farbe und riecht leicht nach Hydro− gensulfat. Die biologische Aktivität wurde in Organbädern an glatten Muskelfasern des Meerschweinchenmagens gemessen. Ergebnisse: Die In−vitro−Versuche zeigen, dass wässeriger Schlammextrakt aus den Seen von Pomorie anregenden Wirkun− gen auf die spontane kontraktile Aktivität glatter Muskelfasern des Meerschweinchenmagens aufweist. Die kontraktile Anre− gung ist durch Huminsubstanzen, die sich im Schlamm befinden, bedingt, die agonistische Wirkungen auf a 2 −Adreno− und D 2 − Dopaminrezeptoren an den glatten Muskelzellen besitzen. Schlussfolgerung: Die erhaltenen Ergebnisse machen klinische Beobachtungen der Anwendung von Heilschlamm verständli− cher und bilden die Grundlage für gezielte klinische Studien, die die Rekrutierung von Labordaten und klinischen Daten zur Heil− schlammtherapie erm−glichen.
AbstractPurpose: Peat and mud have been successfully applied for a vari− ety of clinical indications. Quite apart from the physical−thermal effects, new studies point towards the so called ¹chemical ef− fects" of peat/mud containing substances. These effects include a stimulatory response of the spontaneous contractile activity of smooth muscle tissue. The aim of the present work is to study the extent to which the water mud extract possesses biological activity and the extent to which it resembles the properties of water peat extract. Methods: The studies were carried out with curative Pomorie mud from the black layer, with very fine struc− ture, plasticity, black colour and a slight smell of hydrogen sul− phide. The biological activity was examined in organ baths by means of smooth muscle fibres of the stomach of guinea pigs. Results: Experiments in vitro show that the water extract from curative mud from the Pomorie Lake possesses excitatory action on the spontaneous contractile activity of smooth−muscle strips of guinea pig stomach. The contractile activation is due to the hu− mic substances contained in the mud, which have an agonistic action on the a 2 −adrenergic and D 2 −dopamine receptors of the smooth−muscle cells. Conclusions: The results obtained allow to explain better some clinical effects of curative mud and to conduct targeted studies in c...
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