generated a series of backcross derived isogenic lines which were evaluated in glasshouse and field conditions. Across 13 experiments the GW2-A1 mutant allele significantly increased thousand grain weight (6.6 %), grain width (2.8 %) and grain length (2.1 %) in tetraploid and hexaploid wheat compared to the wild type allele. In hexaploid wheat, this led to an increase in spike yield since no differences were detected for spikelet or grain number between isogenic lines. The increase in grain width and length was consistent across grains of different sizes, suggesting that the effect of the mutation is stable across the ear and within spikelets. Differences in carpel size and weight between alleles were identified as early as 5 days before anthesis, suggesting that TaGW2-A1 acts on maternal tissue before anthesis to restrict seed size. A single nucleotide polymorphism marker was developed to aid the deployment of the mutant allele into breeding programmes.
Selenium (Se) is an essential trace element for humans and livestock. In the UK, human Se intake and status has declined since the 1980s. This is primarily due to the increased use of wheat (Triticum aestivum L.) grown in UK soils which are naturally low in Se. The aim of this study was to determine the potential for increasing grain Se concentration in a high-yielding UK wheat crop using fertilisers. The crop response of winter-wheat to Se fertilisation was determined under standard field conditions in two consecutive years at up to 10 sites. Selenium fertilisers were applied as high-volume drenches of sodium selenate solution, or as granular Se-containing products. Yield and harvest index were unaffected by Plant Soil (2010) 332:5-18
BackgroundGrain yield in wheat is a polygenic trait that is influenced by environmental and genetic interactions at all stages of the plant’s growth. Yield is usually broken down into three components; number of spikes per area, grain number per spike, and grain weight (TGW). In polyploid wheat, studies have identified quantitative trait loci (QTL) which affect TGW, yet few have been validated and fine-mapped using independent germplasm, thereby having limited impact in breeding.ResultsIn this study we identified a major QTL for TGW, yield and green canopy duration on wheat chromosome 6A of the Spark x Rialto population, across 12 North European environments. Using independent germplasm in the form of BC2 and BC4 near isogenic lines (NILs), we validated the three QTL effects across environments. In four of the five experiments the Rialto 6A introgression gave significant improvements in yield (5.5%) and TGW (5.1%), with morphometric measurements showing that the increased grain weight was a result of wider grains. The extended green canopy duration associated with the high yielding/TGW Rialto allele was comprised of two independent effects; earlier flowering and delayed final maturity, and was expressed stably across the five environments. The wheat homologue (TaGW2) of a rice gene associated with increased TGW and grain width was mapped within the QTL interval. However, no polymorphisms were identified in the coding sequence between the parents.ConclusionThe discovery and validation through near-isogenic lines of robust QTL which affect yield, green canopy duration, thousand grain weight, and grain width on chromosome 6A of hexaploid wheat provide an important first step to advance our understanding of the genetic mechanisms regulating the complex processes governing grain size and yield in polyploid wheat.
SUMMARYFor 4 successive years, cultivars of winter wheat differing in resistance to eyespot (Cercosporella herpotrichoides) and to lodging were grown in plots in which different levels of eyespot were induced by inoculating at different times. Lodging was either allowed, or prevented by growing the plants through nets. Yield losses were greatest in the most severely infected plots in which lodging was allowed; losses were reduced where infection was less severe or where lodging was prevented. In the year in which eyespot was most severe, there was a significant regression of yield on disease severity, even for the plots in which lodging was prevented; where lodging was allowed, regression lines were steeper for the lodging susceptible cultivars, but did not differ for the lodging resistant cultivars. Eyespot reduced the number of tillers, measured in spring, but later there was partial compensation for these losses. Grain number per ear and 1000 grain weight of individual tillers were reduced by severe eyespot infection; moderate infection reduced only 1000 grain weight; slight infection caused no reduction. Tillers without severe infection produced extra grains in severely diseased plots, so that there was no overall effect of eyespot on grain number per ear. The results are discussed in relation to the breeding of dwarf cultivars, which may be highly resistant to lodging but can nevertheless suffer severe losses from eyespot.
The oat (Avena sativa L.) breeding program at the Eastern Cereal and Oilseed Research Centre of Agriculture & Agri‐Food Canada has the responsibility to breed new oat cultivars for producers in eastern Canada, which includes Ontario, Quebec, and the Atlantic provinces. A 3‐yr multilocation test was conducted to understand the genotype × location interaction patterns and the relationships among test locations in eastern Canada. A genotype + genotype × environment interaction biplot analysis of yield data revealed three distinct oat mega‐environments in eastern Canada: (i) northern Ontario, (ii) southern and eastern Ontario, and (iii) Quebec and Atlantic Canada. To breed for all mega‐environments, initial yield screening must be conducted at locations representing each of these mega‐environments. Based on the relationships among test locations, six essential test locations were identified: three in Ontario, two in Quebec, and one in Atlantic Canada. Testing at all six locations appeared to provide a good coverage of the whole oat‐growing area in eastern Canada. Based on these findings, a breeding and test strategy was developed. This includes conducting initial yield screening at three locations in Ontario, Quebec, and Atlantic Canada, followed by a formal yield test at all six essential test locations. Specifically adapted genotypes selected from this test will then be tested in the Registration Tests in their respectively adapted subregions.
Flower development is an important determinant of grain yield in crops. In wheat (Triticum spp.), natural variation for the size of spikelet and floral organs is particularly evident in Triticum turgidum ssp. polonicum (also termed Triticum polonicum), a tetraploid subspecies of wheat with long glumes, lemmas, and grains. Using map-based cloning, we identified VEGETATIVE TO REPRODUCTIVE TRANSITION 2 (VRT2), which encodes a MADS-box transcription factor belonging to the SHORT VEGETATIVE PHASE family, as the gene underlying the T. polonicum long-glume (P1) locus. The causal P1 mutation is a sequence rearrangement in intron-1 that results in ectopic expression of the T. polonicum VRT-A2 allele. Based on allelic variation studies, we propose that the intron-1 mutation in VRT-A2 is the unique T. polonicum subspecies-defining polymorphism, which was later introduced into hexaploid wheat via natural hybridizations. Near-isogenic lines differing for the P1 locus revealed a gradient effect of P1 across spikelets and within florets. Transgenic lines of hexaploid wheat carrying the T. polonicum VRT-A2 allele show that expression levels of VRT-A2 are highly correlated with spike, glume, grain, and floral organ length. These results highlight how changes in expression profiles, through variation in cis-regulation, can affect agronomic traits in a dosage-dependent manner in polyploid crops.
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