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Isolates of the fish parasitic copepod Lernaea from four different freshwater fishes viz., catla (Catla catla), guppy (Poecilia reticulata), platy (Xiphophorus naculatus) and gold fish (Carassius auratus) were selected for the study. The isolates had considerably differing anchor shapes and were likely to be grouped as different species. Partial nucleotide sequences of the 18S and 28S rDNA regions of the Lernaea isolates were determined. Phylogenetic trees generated by the neighbour joining method revealed that the Lernaea isolates examined in this study were of similar genotype and were identified to be Lernaea cyprinacea.

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Biofilm of Aeromonas hydrophila oral vaccine was evaluated on growth and histopathological features of striped catfish, Pangasianodon hypophthalmus. Fish were fed with feed incorporating biofilm (BF) or free cell (FC) of A. hydrophila vaccine at 1010 cells/fish/day for 20 days and basal feed for 60 days. From 30th day onwards, till end of the trial, the mean weight gain (g/fish) was significantly higher (P less than 0.05) in BF treated group than both FC and control group. At the end of the study, histopathological changes in gills, liver and kidney were evaluated using haematoxylin eosin technique. The histopathological findings, in BF fed P. hypophthalmus, showed normal architechture of the gills, liver and kidney. However, fishes fed on FC and control group, resulted several histopathological abnormalities in all the organs.

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