Evaluation of biofilm of Vibrio anguillarum for oral vaccination of Asian seabass, Lates calcarifer (BLOCH, 1790)

Ram, Mohit Kumar; Kumar, B. V. Sunil; Poojary, Sathish Rama; Abhiman, P. B.; Patil, Prakash; Ramesh, K.; Shankar, K. M.

doi:10.1016/j.fsi.2019.09.053

Cited by 14 publications

(9 citation statements)

References 18 publications

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“…We conclude that the biofilm vaccine stimulates antibody production from GALT. Other studies have shown that Vibrio anguillarum, Aeromonas hydrophila, and Streptococcus agalactiae biofilm vaccines can increase host antibody production [16,18,45,46].…”

Section: Discussionmentioning

confidence: 99%

“…When oral vaccines are delivered via the gut, local and systemic immune responses will be elicited, which are reflected in high amounts of circulating immunoglobulin M (IgM) [12][13][14]. Oral biofilm vaccines have been confirmed to have protective effects for many fish, such as Clarias batrachus [15], Lates calcarifer [16], Labeo rohita [17], Oreochromis sp. [18], Cyprinus carpio [11,19], and Mugil cephalus [20].…”

Section: Introductionmentioning

confidence: 99%

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Protective Efficacy of Novel Oral Biofilm Vaccines against Photobacterium damselae subsp. damselae Infection in Giant Grouper, Epinephelus lanceolatus

Chen

2022

Vaccines

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Photobacterium damselae subsp. damselae is a pathogen that mainly infects a variety of fish species. There are many antibiotic-resistant strains of Photobacterium damselae subsp. damselae. In a previously published article, we described the production method for a novel oral biofilm vaccine. In the study reported herein, we confirmed the protective effect of the oral biofilm vaccine against Photobacterium damselae subsp. damselae. Twenty-eight days after vaccination, phagocytosis increased by 256% relative to the control group. The mean albumin–globulin ratios of the vaccine groups were significantly lower than the mean albumin–globulin ratios of the control group. There were no significant intergroup differences in lysozyme activity. Mean IgM titers were significantly higher in the vaccine group than in the control group. There was a significant upregulation of the TLR 3, IL-1β, and IL-8 genes in the spleen 28 days after vaccination. The cumulative mortality of the control fish was 84% after challenging fish with the Photobacterium damselae subsp. damselae, while the cumulative mortality of the oral biofilm vaccine (PBV) group was 32%, which was significantly higher than those of the whole-cell vaccine (PWV) and chitosan particle (CP) groups. There is minimal published research on the prevention and treatment of Photobacterium damselae subsp. damselae infection; therefore, this oral biofilm vaccine may represent a new method to fill this gap.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protective Efficacy of Novel Oral Biofilm Vaccines against Photobacterium damselae subsp. damselae Infection in Giant Grouper, Epinephelus lanceolatus

Chen

2022

Vaccines

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“…Recent methods for developing oral biofilm vaccines mainly use chitin flakes, and have achieved good protective effects in a variety of fish species, such as common carp ( Cyprinus carpio ) [ 12 , 15 ], catfish ( Clarias batrachus ) [ 16 ], rohu ( Labeo rohita ) [ 17 ], red hybrid tilapia ( Oreochromis sp.) [ 18 ] and Asian seabass ( Lates calcarifer ) [ 19 ]. In addition, oral vaccines use chitosan polymer-based, coated and inactivated bacteria to enhance protective efficacy [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Protective Efficacy of Novel Oral Biofilm Vaccines against Lactococcus garvieae Infection in Mullet, Mugil cephalus

Chen

2021

Vaccines

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Lactococcus garvieae (L. garvieae) is an important pathogen that causes enormous economic losses in both marine and freshwater aquaculture. At present, antibiotics are the only option for farmers to reduce the losses caused by L. garvieae. However, the usage of antibiotics leads to environmental pollution and the production of drug-resistant strains of bacteria. Therefore, vaccination is preferred as an alternative method to prevent infectious diseases. In this study, we describe an effective approach to the production of an oral biofilm vaccine, using bacteria grown on chitosan particles to form biofilms, and thus providing an inactive pathogen that enhances the immune response in fish. We observed the formation of a biofilm on chitosan particles and administered the novel oral biofilm vaccine to fish. We analyzed the immune responses, including antibody production, phagocytic ability, albumin/globulin ratio and immune-related genes, of vaccinated and control groups of black mullet. Our results show that the phagocytic ability of the biofilm vaccine group was 84%, which is significantly higher than that of the control group, and the antibody production in this group was significantly higher compared with the other group. The mRNA expression levels of immune-related genes (TLR2, IL-1β, TNF-α) were significantly upregulated in the spleen after vaccination. In challenge experiments, the relative percent survival (RPS) was 77% in the biofilm vaccine group, 18% in the whole-cell vaccine group, and 0% in the chitosan particle group at 32 days post-vaccination. In addition, we also found that the relative percent survival (RPS) at 1 day post-vaccination was 74% in the biofilm vaccine group, 42% in the whole-cell vaccine group, and 26% in the chitosan particle group. In both long-term and short-term challenge experiments, the viability of the biofilm vaccine group was significantly higher than that of the whole-cell, chitosan particle and PBS groups. We conclude that based on its protective effect, the L. garvieae biofilm vaccine is better than the whole-cell vaccine when challenged several weeks after vaccination. In addition, the biofilm vaccine also has a greater protective effect than the whole-cell vaccine when challenged immediately after vaccination. Therefore, the biofilm vaccine might represent a novel method for the prevention and treatment of L. garvieae infection.

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“…Alternatively, the biofilm‐forming properties of some pathogenic bacteria provide superior efficacy to vaccines based on free cell culture, as demonstrated in the experiments with Asian sea bass ( Lates calcarifer ) immunized by a biofilm of inactivated Vibrio anguillarum 52 …”

Section: Strategies To Increase Oral Vaccines Efficiencymentioning

confidence: 99%

Molecular farming: Expanding the field of edible vaccines for sustainable fish aquaculture

Mičúchová

Piačková

Frébort

et al. 2022

Reviews in Aquaculture

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Vaccines represent one of the most promising strategies for mitigating economic losses imposed by infectious diseases to global aquaculture. While the majority of commercial vaccines employ injection as the main route of delivery, a substantial body of research explores other avenues for the stimulation of protective immunity. Oral vaccines, representing the most favourable and convenient solution for the aquaculture industry, have been at the forefront of scientific interest for the last couple of decades. The orchestrated effort resulted in identifying the main roadblocks on the path to an ideal edible vaccine and provided several ingenious solutions improving the activation of immunity, ensuring the stability of administered antigens and their uptake upon the passage through the stomach. In the presented review, we describe advances in the available knowledge of the processes prerequisite for developing protective mucosal vaccines and focus readers' attention on the untapped potential of plant‐based production systems in this effort. We propose that these approaches not only meet production demands but also fulfil all requirements of an oral vaccine, addressing all the obstacles, previously solved via separate strategies, in one platform. Thus, combined with the available knowledge of molecular adjuvants and produced for a fraction of the cost, plant‐based production, so‐called molecular farming, is an ideal candidate for vaccine development, paving the road to sustainable aquaculture.

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Evaluation of biofilm of Vibrio anguillarum for oral vaccination of Asian seabass, Lates calcarifer (BLOCH, 1790)

Cited by 14 publications

References 18 publications

Protective Efficacy of Novel Oral Biofilm Vaccines against Photobacterium damselae subsp. damselae Infection in Giant Grouper, Epinephelus lanceolatus

Protective Efficacy of Novel Oral Biofilm Vaccines against Photobacterium damselae subsp. damselae Infection in Giant Grouper, Epinephelus lanceolatus

Protective Efficacy of Novel Oral Biofilm Vaccines against Lactococcus garvieae Infection in Mullet, Mugil cephalus

Molecular farming: Expanding the field of edible vaccines for sustainable fish aquaculture

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