Effect of Biofilm of Aeromonas Hydrophila Oral Vaccine on Growth Performance and Histopathological Changes in Various Tissues of Striped Catfish, Pangasianodon Hypophthalmus (Sauvage 1878)

Abstract

Biofilm of Aeromonas hydrophila oral vaccine was evaluated on growth and histopathological features of striped catfish, Pangasianodon hypophthalmus. Fish were fed with feed incorporating biofilm (BF) or free cell (FC) of A. hydrophila vaccine at 1010 cells/fish/day for 20 days and basal feed for 60 days. From 30th day onwards, till end of the trial, the mean weight gain (g/fish) was significantly higher (P less than 0.05) in BF treated group than both FC and control group. At the end of the study, histopathological changes in gills, liver and kidney were evaluated using haematoxylin eosin technique. The histopathological findings, in BF fed P. hypophthalmus, showed normal architechture of the gills, liver and kidney. However, fishes fed on FC and control group, resulted several histopathological abnormalities in all the organs.

Histopathology of head kidney tissues in challenged rohu, Labeo rohita Hamilton after vaccinating with Aeromonas hydrophila antigens

“…Mamun et al. [34] also observed several histopathological observations like destruction of Bowman's space, inflammatory exudate, glomerular necrosis, tubular necrosis and infiltration of leukocyte cells in the kidney of striped catfish, Pangasianodon hypophthalmus while they fed with A. hydrophila vaccine.

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Evaluation of feed utilization, immune response and disease resistance in striped catfish, Pangasianodon hypophthalmus (Sauvage 1878) fed with a novel Aeromonas hydrophila biofilm vaccine

“…[ 67 ]. The histopathological findings in the present study, in BF fed P. hypophthalmus , showed normal architecture however, several histopathological abnormalities were detected in gills, liver and kidney of FC and control group fishes [ 75 ]. Our results also corroborate the finding of Biswas et al.…”

Evaluation of three alum-precipitated Aeromonas hydrophila vaccines administered to Labeo rohita, Cirrhinus mrigala and Ctenopharyngodon idella: immunokinetics, immersion challenge and histopathology

“…These findings are in congruence with a previous study from India in which histopathological findings in P. hypophthalmus showed normal architecture of the gills, liver and kidney after oral A. hydrophila vaccination. However, fishes in control group, resulted several histopathological abnormalities in all the organs (Mamun et al, 2020).…”