The presented paper deals with monitoring of the ways of selenium (Se) content increase by foliar biofortification with inorganic Se in two varieties of garden pea Pisum sativum L. (Premium, Ambassador) in the conditions of south Slovakia in 2014-2015. The results of experiments show that treatment of plants with two doses of Se concentration (50 g and 100 g Se ha -1 in the form of a sodium selenate anhydrous solution) at the flowering stage significantly increased the total Se content in the seeds of both varieties. Following the results by the consumption of 25 g of dried seeds of peas or 100 g of fresh pea seeds after the biofortification with 100 g Se ha -1 a recommended daily dose of Se in humans may be covered. The significantly positive influence of Se application on the total polyphenols content (TPC) has been confirmed in the both varieties after application of dosage in 100 g Se ha -1 (52% and 33%). A significant increase in the average value of total antioxidant capacity (TAC by DPPH method) in garden pea var. Ambassador was observed after the application of both doses of Se, in case of Premium variety only after application with a 100 g Se ha -1 . Significantly increasing level of TAC by PCL (photochemiluminescence) method was found out only in case of var. Premium.
In this study, electrothermal atomic absorption spectrometry (ETAAS) was used for determination of lead and cadmium in Macedonian white wines. Wine samples, without prior purification, were directly injected into the ETAAS system, using matrix modifiers for Pb and Cd. The standard addition method was used for quantitative analysis of Pb and Cd content. Reliability of the method was verified by determination of selected validation characteristics. The results of the measurements indicated satisfactory precision and accuracy, confirming that the method is accurate and convenient for quantitative analysis. The lead and cadmium concentrations were ranged between 2-28.5 and 0.4-5.69 μg/L, respectively. The levels were below the maximal allowed concentration in wine and compare well with those reported for similar wines from other parts of the world.
The species Pleurotus ostreatus is a commercially, gastronomically, and biotechnologically important fungus. Its strain variability has been little researched. The study provides an evaluation of 59 oyster mushroom production strains in terms of the ability to accumulate selected metals in the cap and stipe. The fruiting bodies were grown under identical model conditions on straw substrate. Metal concentrations (ET-AAS) in dry fruiting bodies ranged in values 1.7–22.4 mg kg−1 for Al, 2.6–9.7 mg kg−1 Ba, 199–4560 mg kg−1 Ca, 1.7–12.0 mg kg−1 Cu, 12–120 mg kg−1 Fe, 16,000–49,500 mg kg−1 K, 876–2400 mg kg−1 Mg, 0.39–11.0 mg kg−1 Mn, 46–920 mg kg−1 Na and 11–920 mg kg−1 for Zn. More Cu, Fe, K, Mg, Mn, Zn accumulated in the cap, while in the stipe Ba was amassed. No significant difference was found between Al, Ca and Na between the accumulation in the cap and the stipe. Furthermore, the dependence of metal uptake from the substrate depending on the fortification of the substrate was confirmed. Statistically significant (p < 0.05) synergistic relationships were shown in pairs Al and Ba, Al and Fe, Ba and Na, Ba and Ca, Ca and Na, Cu and Fe, Fe and Mn, Fe and Zn, K and Mg, K and Mn, K and Zn, Mg and Mn, Mg and Na, Mg and Zn and Mn and Zn in the substrate without the addition of sodium selenate to the substrate. Altered relationships were observed after the application of sodium selenate to the substrate, synergism of Se and Ni, Se and Co and Se and Hg, Cu and Mn, Cu and Fe, Zn and Co, Zn and Ni, Zn and Hg, Mn and Fe, Mn and Cr, Co and Ni, Co and Hg, Ni and Hg, Pb and Cd. The findings of the study may help in the selection of production strains with hypercumulative properties for a particular metal and subsequent use in the addition of fortified fruiting bodies (e.g., with Zn). Based on the study the strains less sensitive to the accumulation of hazardous metals is possible to select for large-scale production, which is important from the perspective of food safety.
The effect of genotype and selenium foliar biofortification in the form of an aqueous solution of sodium selenate on the content of total carotenoids, vitamin C, total polyphenols and selenium content in the tomato fruits was studied. Field experiment was held in the Botanical garden of the Slovak University of Agriculture in 2016. Seven determinant varieties of tomato in the two variants were observed. The results of experiments show that treatment of plants with the dose of Se concentration (150 g Se.ha-1) at the flowering stage significantly increased the total Se content in the in tomato fruits. Foliar application of selenium had a positive effect on the increase of total polyphenol. The influence of Se biofortification on the content of vitamin C and carotenoids was not detected. Selenium foliar fertilization in dosage 150 g.ha-1 is suitable way of tomato fruits enriching in polyphenols, without negative effect on other antioxidants content.
The work aimed to determine the potential of selenium incorporation into seeds of selected species of Ocimum spp. after fortification with a foliar solution of sodium selenate at a concentration of 50 g Se · ha−1. In a 2-year trial, the selenium content was determined by electrothermal atomic absorption method with Zeeman background correction. Modified spectrophotometric method (2,2-diphenyl-1-picrylhydrazyl [DPPH] assays) was used to rate the potential of oxidation– reduction components of basil seeds (AA). The total polyphenol content (TPC) was determined spectrophotometrically using the Folin–Ciocalteu reagent and gallic acid (GA) as the standard solution. The results of experiments showed that the selenium biofortification significantly (p < 0.05) increased the content of selenium in basil seeds (17-fold increase in comparison with controlled variant in case of Tulsi, 12-fold in ‘Cinamonette’ and 12-fold in ‘Dark Green’ when compared with control). The basil seeds represented a valuable source of polyphenols (1414.61–1681.75 μg GA · g−1 dried weight [d.w.]) with multiple times higher antioxidant activity (23.50–28.97 mmol Trolox · kg−1) in comparison with common tested horticultural crops (e.g. peas, tomato and pumpkin). Significant influence of fortification was not found in AA and TPC values. Fortification was not significantly reflected in AA and TPC values. In addition to its very strong reproductive function, healing and religious purposes, the basil seed is used as a functional food due to its high content of bioactive compounds.
The adsorption of contaminants plays an important role in the process of their elimination from a polluted environment. This work describes the issue of loading environment with lead Pb(II) and the resulting negative impact it has on plants and living organisms. It also focuses on bentonite as a natural adsorbent and on the adsorption process of Pb(II) ions on the mixture of bentonite and bottom sediment from the water reservoir in Kolíňany (SR). The equilibrium and kinetic experimental data were evaluated using Langmuir isotherm kinetic pseudo-first and pseudo-second-order rate equations the intraparticle and surface diffusion models. Langmuir isotherm model was successfully used to characterize the lead ions adsorption equilibrium on the mixture of bentonite and bottom sediment. The pseudo second-order model, the intraparticle and surface (film) diffusion models could be simultaneously fitted the experimental kinetic data.
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