Evaluation of the ET-AAS and HG-AAS methods of selenium determination in vegetables

Hegedűs, Ondrej; Hegedüsová, Alžbeta; Šimková, Silvia; Pavlı́k, V.; Jomová, Klaudia

doi:10.1016/j.jprot.2008.01.002

Cited by 26 publications

(5 citation statements)

References 9 publications

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“…Quantitative determination of Se was done by using of ET-AAS method with Zeeman background correction. Atomic absorption spectrometer SpectrAA240FS (Varian, Mulgrave Virginia, Australia) was used to measure the total selenium content [Hegedűs et al 2008]. Conditions for selenium measurement were set in the equipment according to the recommendations of the manufacturer [Rothery 1988] for ET-AAS technique.…”

Section: Determination Of Qualitative Parametersmentioning

confidence: 99%

Untitled

2017

Acta Sci. Pol. Hortorum Cultus

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The presented paper deals with monitoring of the ways of selenium (Se) content increase by foliar biofortification with inorganic Se in two varieties of garden pea Pisum sativum L. (Premium, Ambassador) in the conditions of south Slovakia in 2014-2015. The results of experiments show that treatment of plants with two doses of Se concentration (50 g and 100 g Se ha-1 in the form of a sodium selenate anhydrous solution) at the flowering stage significantly increased the total Se content in the seeds of both varieties. Following the results by the consumption of 25 g of dried seeds of peas or 100 g of fresh pea seeds after the biofortification with 100 g Se ha-1 a recommended daily dose of Se in humans may be covered. The significantly positive influence of Se application on the total polyphenols content (TPC) has been confirmed in the both varieties after application of dosage in 100 g Se ha-1 (52% and 33%). A significant increase in the average value of total antioxidant capacity (TAC by DPPH method) in garden pea var. Ambassador was observed after the application of both doses of Se, in case of Premium variety only after application with a 100 g Se ha-1. Significantly increasing level of TAC by PCL (photochemiluminescence) method was found out only in case of var. Premium.

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Section: Determination Of Qualitative Parametersmentioning

confidence: 99%

Untitled

2017

Acta Sci. Pol. Hortorum Cultus

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“…A flow injection technique has been described in literature where the oxidation reaction of 3 methyl-2benzothiazolinone hydrazone hydrochloride by selenite followed by coupling reaction with chromotropic acid has been used (Rekha et al, 2006). Apart from spectrophotometric methods, other methods of Se (IV) determination commonly employed include nuclear techniques (Alamin et al, 2006;Yusof, 1997), atomic absorption spectroscopy (Capelo et al, 2006;Hegedus et al, 2008) and inductively coupled mass spectrometry (Chen et al, 2007;Ohki et al, 2016;Jagtap & Maher, 2016), and voltammetry (Devi et al, 2017) etc.…”

Section: Introductionmentioning

confidence: 99%

An optical sensor for selenite determination in aqueous samples

Kulkarni

Ramekar²,

Kulkarni

2018

J Anal Sci Technol

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Background: In the present work, an optical sensor has been developed for the determination of selenium (IV) in aqueous samples. The optical sensor is a cellulose triacetate plasticized membrane with tri-octyl methyl ammonium chloride (Al-336) immobilized in it as an ion exchanger. Methods: The sensor works in two steps. In the first step, a 1 × 2 cm piece of the membrane is allowed to equilibrate with a known amount of selenium (IV) solution and potassium iodide in acidic medium. During this equilibration, the iodometric reaction takes place and, I 3 − (tri-iodide anion) is formed which gets sorbed into the membrane due to which the colorless film changes to yellow color showing an absorption maxima at 355 nm which is a characteristic of I 3 −. In the second step the I 3 − loaded membrane is allowed to equilibrate with a solution of variamine blue dye in presence of sodium acetate. In this step, oxidation of the dye takes place and the membrane shows a color change from yellow to violet and a shift in the absorption maxima from 355 to 550 nm. Results: The method is used to study the quantitative determination of selenium in aqueous samples, where the amount of selenium is varied and the corresponding absorbance at 550 nm is measured. A good linear curve is obtained in the concentration range of 0.2 to 3 μg mL −1 of selenium. Conclusions: The proposed method can be successfully applied to determination of selenium (IV) in various pharmaceutical and environmental samples.

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“…Selenium can be determined by high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) [4], hydride generator atomic absorption spectroscopy (HG-AAS) [5], graphite furnace atomic absorption spectroscopy (GF-AAS) [6], gas chromatography (GC) [7], and various electroanalytical techniques (i.e., anodic stripping voltammetry (ASV), cathodic stripping voltammetry (CSV), and adsorptive stripping voltammetry (AdSV)), which exhibit a high sensitivity and are inexpensive [8].…”

Section: Introductionmentioning

confidence: 99%