This paper investigates the effect of different concentrations of nanostructured materials: fullerene‐like (C60), onion‐like carbon (OLC) and ultradispersed diamonds (UDD) on the formation of multicellular spheroids. The chemical composition and purity of nanomaterials are controlled by Fourier transform infrared spectroscopy. The strength and direction of the impact of nanomaterials on the cell population was assessed using microphotography of tumor multicellular spheroids of breast adenocarcinoma cell line MCF‐7 in vitro and Pearson's correlation coefficient. The results demonstrated that UDD and OLC reduced adhesion and cohesive ability of cells and stimulated generation of cell spheroids of ∼3 × 10−3 mm3 in significant amount. The fullerenes reduced in the main cell adhesion to substrate that led to formation of cell aggregates of ∼5 × 10−3 mm3. The results could be useful for achievement of the directed cell growth in three‐dimensional culture.
Research in the field of music anthropology as a separate science began to appear only in the last century. Currently, there are only a small number of studies on musical anthropology in the scientific literature. The purpose of the study is a theoretical generalisation of the manifestations of anthropological thinking in the musical art of classicism, romanticism, and avant-garde, carried out on the basis of contemporary scientific publications. The research methodology is based on the principles of analytical, historical, cultural, and comparative methods, which cover the aspects of musical anthropology of different eras. During the study, the authors considered the manifestations of anthropological thinking in the musical art of classicism, romanticism, and avant-garde, as well as modern scientific publications on musical anthropology. The authors concluded that synthetic, epic-dramatic genres of poetic ballads and poems had a particular influence on the musical composition of the Romantic era. These genres led to the emergence of a number of new compositional structures in the instrumental music of the Romanticism. This study can be used during lectures and practical classes in higher education institutions that specialise in the study of music; it can also be used by students for independent study of musical anthropology.
This paper describes the impact of different concentrations of single-walled carbon nanotubes (SWCNTs) on cell viability of breast adenocarcinoma, MCF-7 line, and formation of multicellular tumor spheroids (MTS). Chemical composition and purity of nanotubes is controlled by Fourier transform infrared spectroscopy. The strength and direction of the influence of SWCNTs on the tumor cell population was assessed by cell counting and measurement of the volume of multicellular tumor spheroids. Effect of SWCNTs on the formation of multicellular spheroids was compared with the results obtained by culturing tumor cells with ultra dispersed diamonds (UDDs). Our results demonstrated that SWCNTs at concentrations ranging from 12.5 to 50 μg/ml did not have cytotoxic influence on tumor cells; instead, they had weak cytostatic effect. The increasing of SWCNTs concentration to 100 to 200 μg/ml stimulated proliferation of tumor cells, especially in suspension fractions. The result of this influence was in formation of more MTS in cell culture with SWCNTs compared with UDDs and control samples. In result, the median volume of MTS after cultivation with SWCNTs at 100 to 200 μg/ml concentrations is 3 to 5 times greater than that in samples which were incubated with the UDDs and is 2.5 times greater than that in control cultures. So, if SWCNTs reduced cell adhesion to substrate and stimulated formation of tumor cell aggregates volume near 7 · 10−3 mm3, at the same time, UDDs reduced adhesion and cohesive ability of cells and stimulated generation of cell spheroids volume no more than 4 · 10−3 mm3. Our results could be useful for the control of cell growth in three-dimensional culture.PACS61. 46 + w; 61.48 + c; 61.48De; 87.15-v; 87.64-t
The actual task of oncology is effective treatment of cancer while causing a minimum harm to the patient. The appearance of polymer nanomaterials and technologies launched new applications and approaches of delivery and release of anticancer drugs. The goal of work was to test ultra dispersed diamonds (UDDs) and onion-like carbon (OLCs) as new vehicles for delivery of antitumor drug (doxorubicin (DOX)) and specific antibodies to tumor receptors. Stable compounds of UDDs and OLCs with DOX were obtained. As results of work, an effectiveness of functionalization was 2.94 % w/w for OLC-DOX and 2.98 % w/w for UDD-DOX. Also, there was demonstrated that UDD-DOX and OLC-DOX constructs had dose-dependent cytotoxic effect on tumor cells in the presence of trypsin. The survival of adenocarcinoma cells reduced from 52 to 28 % in case of incubation with the UDD-DOX in concentrations from 8.4–2.5 to 670–20 μg/ml and from 72 to 30 % after incubation with OLC-DOX. Simultaneously, antibodies to epidermal growth factor maintained 75 % of the functional activity and specificity after matrix-assisted pulsed laser evaporation deposition. Thus, the conclusion has been made about the prospects of selected new methods and approaches for creating an antitumor agent with capabilities targeted delivery of drugs.
The objective of this research is to develop the equipment for liquid-jet forming of women's headwear hats, which will allow expanding design assortment of these products. The equipment for cyclic liquid-jet forming of headwear hats in liquid-active working environment (LAWE) with forming element rotation was designed. The mechanism of movement and orientation of liquid -jet forming nozzle was developed, which allows influencing fabric parts by flooded and controlled liquid-jet, repeating the contour at a certain distance and with appropriate angle attack on each technological section of the part. The conducted experimental research allowed to receive semi-spherical forms of headwear hats from six fabrics suit-coat assortments, which are one of the most difficult forms of non-seam forming. Completely formed hats of a given complex configuration were obtained by liquid-jet forming, different from standard semi-spherical ones. The developed equipment provides mobility of the production from the point of view of quick equipment adaptation due to action use of flooded controlled liquid-jet and forming of a significant number of various hats contours, various combinations of technological forming modes.
New trends are emerging in the system of higher art education due to globalisation and integration, rapid development of technological innovations. The use of technological innovations causes emerging of new types of communication, collaboration and interaction between teachers and students. In the article, the concept of smart education and its principles and the competence of a future teacher and specifics of their professional training were described. Global educational trends were characterised. The perspectives of professional and pedagogical activity of art teacher were outlined. The importance of technological innovations in the process of innovative education was explained. The criteria for assessing the teacher's innovative competence were highlighted. It was established that the modern smart society requires a teacher who teaches art history while constantly developing his cognitive abilities, systematically renew his intellectual and creative potential. The peculiarities of teacher's innovative competence formation in the changing conditions of a modern “smart” society were investigated. It was concluded that digital competence is one of the new requirements for art critics training in the context of the development of the information-oriented society.
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