Abstract:This paper describes the impact of different concentrations of single-walled carbon nanotubes (SWCNTs) on cell viability of breast adenocarcinoma, MCF-7 line, and formation of multicellular tumor spheroids (MTS). Chemical composition and purity of nanotubes is controlled by Fourier transform infrared spectroscopy. The strength and direction of the influence of SWCNTs on the tumor cell population was assessed by cell counting and measurement of the volume of multicellular tumor spheroids. Effect of SWCNTs on th… Show more
“…Compared to N0, the band width increased in the O-H stretching region at the peak at 3300 cm −1 in N2, N3 and N5. 28–31,34,35 Figure 15.FTIR-ATR spectra of N0 sample and N2, N3 and N5 samples.…”
Section: Resultsmentioning
confidence: 99%
“…Compared to N0, the bandwidth increased in the O-H stretching region at the peak at 3300 cm À1 in N1, N2, N3 and N4. [28][29][30][31][32][33][34][35] The bands of tension and bending vibrations were examined from the FTIR-ATR graph in Figure 14. The new peaks observed as a result of the examination are the peak at 839 cm À1 in N5, N6, N7 and N8 compared to N0; comes from the active peak of graphite.…”
Section: Investigation Of the Chemical (Ftir-atr) Condition Of The Su...mentioning
confidence: 99%
“…Compared to N0, the band width increased in the O-H stretching region at the peak at 3300 cm À1 in N2, N3 and N5. [28][29][30][31]34,35…”
Section: Investigation Of the Chemical (Ftir-atr) Condition Of The Su...mentioning
Composite materials have many features to contribute to aircraft design but have low electrical conductivity. In this study, it is aimed to increase the electrical conductivity of FRP (Fiber Reinforced Polymer) composites used in different parts of the aircraft. The modified nanoparticles were added into the aircraft paint and applied on the composites used in the radome part of the aircraft, and it is aimed to either increase or keep the electromagnetic permeability stable. Firstly, the surfaces of the FRP composite samples were painted by Graphene and Double-Walled Carbon Nanotube (DWCNT) to reinforce the conductive nanoparticles at different ratios. Resistance measurements of the samples were made according to the Airbus “Electrical Surface Resistance Measurement” procedure and their electrical conductivity was calculated. The Non-Contact Measurement Method test setup was used to obtain information about the electromagnetic properties of the samples. As a result of the measurements, N3 (σ = 4.37x10−4 S/m, S21 = −15.81 dB) sample conforms the best with the objectives of ourstudy compared to electrical conductivity (N0, σ = 4.83x10−5 S/m) and electromagnetic permeability (N0, S21 = −15.83 dB) of the reference sample. The contact angle measurements were taken to determine the contact angle changes depending on the surface morphology and conductivity changes of the samples painted with different and varying amounts of nanoparticle doped paint. Additionally, measurements were taken with the FTIR-ATR instrument to make surface analysis of the samples.
“…Compared to N0, the band width increased in the O-H stretching region at the peak at 3300 cm −1 in N2, N3 and N5. 28–31,34,35 Figure 15.FTIR-ATR spectra of N0 sample and N2, N3 and N5 samples.…”
Section: Resultsmentioning
confidence: 99%
“…Compared to N0, the bandwidth increased in the O-H stretching region at the peak at 3300 cm À1 in N1, N2, N3 and N4. [28][29][30][31][32][33][34][35] The bands of tension and bending vibrations were examined from the FTIR-ATR graph in Figure 14. The new peaks observed as a result of the examination are the peak at 839 cm À1 in N5, N6, N7 and N8 compared to N0; comes from the active peak of graphite.…”
Section: Investigation Of the Chemical (Ftir-atr) Condition Of The Su...mentioning
confidence: 99%
“…Compared to N0, the band width increased in the O-H stretching region at the peak at 3300 cm À1 in N2, N3 and N5. [28][29][30][31]34,35…”
Section: Investigation Of the Chemical (Ftir-atr) Condition Of The Su...mentioning
Composite materials have many features to contribute to aircraft design but have low electrical conductivity. In this study, it is aimed to increase the electrical conductivity of FRP (Fiber Reinforced Polymer) composites used in different parts of the aircraft. The modified nanoparticles were added into the aircraft paint and applied on the composites used in the radome part of the aircraft, and it is aimed to either increase or keep the electromagnetic permeability stable. Firstly, the surfaces of the FRP composite samples were painted by Graphene and Double-Walled Carbon Nanotube (DWCNT) to reinforce the conductive nanoparticles at different ratios. Resistance measurements of the samples were made according to the Airbus “Electrical Surface Resistance Measurement” procedure and their electrical conductivity was calculated. The Non-Contact Measurement Method test setup was used to obtain information about the electromagnetic properties of the samples. As a result of the measurements, N3 (σ = 4.37x10−4 S/m, S21 = −15.81 dB) sample conforms the best with the objectives of ourstudy compared to electrical conductivity (N0, σ = 4.83x10−5 S/m) and electromagnetic permeability (N0, S21 = −15.83 dB) of the reference sample. The contact angle measurements were taken to determine the contact angle changes depending on the surface morphology and conductivity changes of the samples painted with different and varying amounts of nanoparticle doped paint. Additionally, measurements were taken with the FTIR-ATR instrument to make surface analysis of the samples.
“…As for SPEV, the high concentration of AuNPs may have a cytostatic effect which reduced cell numbers in the adhesive fraction and the number of MSs in suspension. Previously, the authors reported that carbon nanoparticles reduce the adhesion of cells to the substrate, stimulate cell transfer into the suspension, and leaded to formation of multicellular spheroids [ 25 , 26 ]. In the literature, there are data about AuNP ability to break the structure of actin/myosin microfilaments and decrease cell proliferation, adhesion, and differentiation [ 27 ].…”
Section: Resultsmentioning
confidence: 99%
“…The multicellular spheroids (MSs) were generated to estimate the in vitro impact of AuNPs on migration and aggregation potential of investigated cells. Spheroid (3-D) model system of SPEV and HT29 cells was cultured by the conventional method, which was reported by [ 24 ] and modified in our laboratory [ 25 ]. Briefly, cell suspension were counted using trypan blue and equal numbers of cells (5 × 10 4 cells/cm 2 ) were planted in full supplemented culture medium.…”
Cell culture models are excellent tools for potential toxicity of nanoparticles and fundamental investigations in cancer research. Thus, information about AuNP potential toxicity and effects on human health is necessary for the use of nanomaterials in clinical settings. The aim of our research is to examine the effects of AuNPs on the epithelial origin cell lines: continuous and oncogenic. Embryonic porcine kidney epithelial inoculated (SPEV) cell line and colorectal carcinoma cell line (HT29) were used. In the test cultures, the cell proliferation, necrosis/apoptosis, and multicellular spheroids generation were evaluated. We demonstrated that AuNP concentrations of 6–12 μg/ml reduced the proliferation of SPEV and HT29 cells and increased the cell number at early and late stages of apoptosis and necrosis. It was shown that small concentrations of AuNPs (1–3 μg/ml) stimulate multicellular spheroid formation by HT29 and SPEV cells. However, higher AuNP concentrations (6–12 μg/ml) had both cytotoxic and anti-cohesive effects on cell in suspension. The large sensitiveness to the action of AuNPs was shown by the line of HT29 (6 μg/ml) as compared to the SPEV cells (12 μg/ml). This experimental study of the effect of AuNPs on SPEV and HT29 cell lines will justify their further application in AuNP-mediated anticancer treatment.
Regions of hypoxia in tumours can be modelled in vitro in 2D cell cultures with a hypoxic chamber or incubator in which oxygen levels can be regulated. Although this system is useful in many respects, it disregards the additional physiological gradients of the hypoxic microenvironment, which result in reduced nutrients and more acidic pH. Another approach to hypoxia modelling is to use three-dimensional spheroid cultures. In spheroids, the physiological gradients of the hypoxic tumour microenvironment can be inexpensively modelled and explored. In addition, spheroids offer the advantage of more representative modelling of tumour therapy responses compared with 2D culture. Here, we review the use of spheroids in hypoxia tumour biology research and highlight the different methodologies for spheroid formation and how to obtain uniformity. We explore the challenge of spheroid analyses and how to determine the effect on the hypoxic versus normoxic components of spheroids. We discuss the use of high-throughput analyses in hypoxia screening of spheroids. Furthermore, we examine the use of mathematical modelling of spheroids to understand more fully the hypoxic tumour microenvironment.
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