In the industrial pig farm for 26 thousand heads, the analysis of the influence of a forage factor on bacteria carriers of a uterine pig population in connection with mass morbidity of dairy piglets on anaerobic enterotoxemia is carried out. Actinobacillus pleuropneumonia, Pasteurella multocida, Clostridium perfringens, Neisseria spp., Candida albicans, Aspergillus niger which are able to form biofilms, were isolated from five samples of “SK-1” compound feed for pregnant sows and from the blood of animals (n=20) fed with this compound feed. The structural basis of the most stable biofilms in vitro were the aerobic fungi Aspergillus niger and Candida albicans. Biofilm-forming variants of these bacteria showed multidrug resistance to 30 antimicrobial drugs (synthetic penicillins, cephalosporins, fluoroquinolones, aminoglycosides, tetracyclines, combination drugs). Isolates of associative microflora isolated from the blood of sows were pathogenic for 30% of laboratory mice. It was found that probiotic agent No1 (composition based on Bischofite with probiotics) showed the universal bactericidal activity against the bacteria Actinobacillus pleuropneumonia, Pasteurella multocida, Clostridium perfringens, Neisseria spp.
The obtained experimental-analytical data indicate a significant risk of occurrence of circovirus-associated syndrome complexes proliferative necrotic pneumonia and a complex of respiratory diseases of pigs (PNP and PRDC) from the presence of Pasteurella and Pasteurellacea bacteria in barley grain (OR = 3.48; 2.21 < OR < 5.50; p ≤ 0.01 at n = 633) and the critical importance of these bacteria for the clinical manifestation of these syndromes in pig farms of Ukraine, which are seropositive both for PCV-2 and AD (OR = 35.45; 18.12 < OR < 69.35; p ≤ 0.001 at n = 433). Thus, barley grain grown on forage lands “fertilized” with pig manure is a factor in the transmission of at least pasteurellae in the epizootic chains of PNP and PRDC, and thus form a “feed chain” of PCV-2. Prospects for further development of the concept of the food chain of circovirus-associated infections in pig breeding are discussed
The novel coronavirus SARS-CoV-2 that causes COVID-19 disease, was mainly described as a something extraordinary in media discourse. Its “unusual,” almost “mystical” properties have been constantly invented by world media sources since January 2020 thus far. Critical comparison of the real epidemiological, physical, chemical and biological properties of SARS-CoV-2 with most common causative agents of coronavirus family demonstrates that principles and techniques of laboratory diagnostics and prophylaxis of SARS-CoV-2 should be built only on the basis of evidence-based medicine, not on imaginary properties of the virus that were created with ideological purposes.
B.T. Stegniydocteur en sciences vétérinaires, académicien de l'Académie nationale des sciences d'Ukraine, directeur de l'Institut de médecine vétérinaire expérimentale et clinique (Kharkiv)
Research results and discussion. All vaccinated adult mouse (n=12) at contrast to unvaccinated (n=8) were alive after its inoculation with broth culture of Mannheimia haemolytica in dose 0.5 cm 3 (10 5 BC 50) during 1 month of trials. Level of vaccinated mouse seroconversion on PRRS, PCV-2 and bacterial antigens was 0; 1/2.30+0.11 and 1/351.00+5.73 (P<0.01), in correspondence. All vaccinated pigs (n=5) after first injection had a levels of seroconversion on PRRS, PCV-2 and bacterial antigens (3 week after vaccination) 1/8.80+2.05; 1/22.50+7.73 and 1/51.00+6.88 (P<0.05), in correspondence. After revaccination (3 week after first injection) pigs had a levels of seroconversion on PRRS, PCV-2 and bacterial antigens (3 week after second injection) 1/17.40+0.78; 1/34.23+0.73 and 1/121.11+2.44 (P<0.05), in correspondence. Conclusions. Modified vaccine "ReproSuiVac-IECVM" are ready to clinical trials against the concurrent ASF-hemolytic pasteurellosis infections that promote of blind spreading of ASF agent in the quarantine areas of ASF outbreaks (promote of its attribution) in Ukraine.
The paper presents the results on the species and percentage composition of the microflora in biofilms of pig feed, which varies depending on the seasonal factor. Bacteria Streptococcus spp., Pasteurella multocida, Neisseria spp., and Clostridium perfringens in biofilms were found much more often (by 25% or more) in the warm period of the year, while listeria in silage and haylage — in the autumn–winter period. This property of feed biofilms is also significantly influenced by the conditions of cultivation, harvesting and storage of agricultural products. In the study of biofilms of microflora of barley, corn and wheat, it was found that their structural basis are aerobic fungi of the mold Aspergillus spp. Bacteria Streptococcus spp., Pasteurella multocida, Neisseria spp., and Clostridium perfringens without mold form much looser biofilms in vitro and these biofilms are much more sensitive to a wide range of commercial antibiotics. The structural basis of polymicrobial biofilms of barley, corn and wheat microflora is highly likely to be aerobic fungi of Aspergillus spp.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.