In the industrial pig farm for 26 thousand heads, the analysis of the influence of a forage factor on bacteria carriers of a uterine pig population in connection with mass morbidity of dairy piglets on anaerobic enterotoxemia is carried out. Actinobacillus pleuropneumonia, Pasteurella multocida, Clostridium perfringens, Neisseria spp., Candida albicans, Aspergillus niger which are able to form biofilms, were isolated from five samples of “SK-1” compound feed for pregnant sows and from the blood of animals (n=20) fed with this compound feed. The structural basis of the most stable biofilms in vitro were the aerobic fungi Aspergillus niger and Candida albicans. Biofilm-forming variants of these bacteria showed multidrug resistance to 30 antimicrobial drugs (synthetic penicillins, cephalosporins, fluoroquinolones, aminoglycosides, tetracyclines, combination drugs). Isolates of associative microflora isolated from the blood of sows were pathogenic for 30% of laboratory mice. It was found that probiotic agent No1 (composition based on Bischofite with probiotics) showed the universal bactericidal activity against the bacteria Actinobacillus pleuropneumonia, Pasteurella multocida, Clostridium perfringens, Neisseria spp.
Research results and discussion. All vaccinated adult mouse (n=12) at contrast to unvaccinated (n=8) were alive after its inoculation with broth culture of Mannheimia haemolytica in dose 0.5 cm 3 (10 5 BC 50) during 1 month of trials. Level of vaccinated mouse seroconversion on PRRS, PCV-2 and bacterial antigens was 0; 1/2.30+0.11 and 1/351.00+5.73 (P<0.01), in correspondence. All vaccinated pigs (n=5) after first injection had a levels of seroconversion on PRRS, PCV-2 and bacterial antigens (3 week after vaccination) 1/8.80+2.05; 1/22.50+7.73 and 1/51.00+6.88 (P<0.05), in correspondence. After revaccination (3 week after first injection) pigs had a levels of seroconversion on PRRS, PCV-2 and bacterial antigens (3 week after second injection) 1/17.40+0.78; 1/34.23+0.73 and 1/121.11+2.44 (P<0.05), in correspondence. Conclusions. Modified vaccine "ReproSuiVac-IECVM" are ready to clinical trials against the concurrent ASF-hemolytic pasteurellosis infections that promote of blind spreading of ASF agent in the quarantine areas of ASF outbreaks (promote of its attribution) in Ukraine.
At different stages of feed production and storage, it is possible to contaminate both feed and their components with various pathogenic and opportunistic microorganisms that can cause infectious diseases not only among animals but also have epidemiological significance. The aim of the study was to isolate biofilm-forming strains of microorganisms from feed, as well as to study the inhibitory activity of the probiotic complex of bacteria of the genus Bacillus against microbial biofilms. Identification and species affiliation of isolated bacterial field isolates were performed by cultural-morphological and biochemical properties. The formation of biofilms was studied by determining the ability of isolates of microbial associations and individual species of microorganisms to adhere to the surface of a 96-well polystyrene tablet according to the method of O'Toole & Kolter, 1998. Determination of microbial contamination of 50 industrial batches of feed from 4 pig farms in two regions of Ukraine (barley, compound feed SK-31 for rearing, SK-51 for fattening pigs, EXCELL starter for pigs 15%, shop prestarter, compound feed for lactating sows). In 11 experimental batches of barley (68.8%) and 13 batches of 3 types of feed (SK-31, SK-51, feed for lactating sows) identified associations with different microorganisms Pasteurella multocida, Corynebacterium striatum, Bacillus subtilis, Leptothrix ochracea, Haemophilus parasuis and yeast Candida albicans. The association of Actinobacillus pleuropneumonia bacteria with B. subtilis was identified in 2 batches (50%) of the shop prestarter. Moderate, by optical density, biofilm formation for associations of microorganisms P. multocida + C. striatum + C. albicans (D620=3.59) and P. multocida + L. ochracea + C. albicans (D620=3.62). Planktonic forms of C. striatum and P. multocida showed low film-forming activity at the level (D620<1.51). Inhibitory activity of the probiotic complex of bacteria of the genus Bacillus (B. subtilis, B. licheniformis, B. amyloliquefaciens) was determined by isolated variations of microbial biofilms in 5 feed species, which displaced all biofilm-forming microorganisms except H. parasuis
The article presents data on the optimization of the clinical protocol for the use of oil and adsorbed forms of experimental vaccine “ReproSuiVac-P” in industrial pig breeding (in the nucleus of herd, in groups of growing and fattening) in the system of biosafety measures “StopASFmix” based on immunological indicators of immunity, including humoral and cellular, with regards of the average daily growth of piglets) and specific (dynamics of postvaccinal antibodies, the intensity of population immunity, the resistance of vaccinated pigs relative to carrier-status of Mannheimia haemolytica). The obtained results allowed, at the level of probability not less than p≤0.01 (n = 879 pigs of different technological groups), to develop a technological card of vaccinations, which provides application of: a) oil form “ReproSuiVac-P” with bacterin on boars, lactating and barren sows twice with an interval 3 weeks in doses of 5+5 sm3; b) oil form of the vaccine in the group of weaned piglets 32nd–35th days of age at a single dose of 1–2 sm3; c) adsorbed with toxoid form of vaccine to sows of the second trimester of gestation twice with an interval of 2 weeks in doses of 5+7 sm3. Based on the indicators of constitutional and specific post-vaccination immunity against associated circovirus-pasteurellosis infection, a technological card of application of experimental vaccine “ReproSuiVac-P” in the main herd and in rearing groups of the industrial pig breeding was developed
The novel coronavirus SARS-CoV-2 that causes COVID-19 disease, was mainly described as a something extraordinary in media discourse. Its “unusual,” almost “mystical” properties have been constantly invented by world media sources since January 2020 thus far. Critical comparison of the real epidemiological, physical, chemical and biological properties of SARS-CoV-2 with most common causative agents of coronavirus family demonstrates that principles and techniques of laboratory diagnostics and prophylaxis of SARS-CoV-2 should be built only on the basis of evidence-based medicine, not on imaginary properties of the virus that were created with ideological purposes.
B.T. Stegniydocteur en sciences vétérinaires, académicien de l'Académie nationale des sciences d'Ukraine, directeur de l'Institut de médecine vétérinaire expérimentale et clinique (Kharkiv)
The paper presents the results on the species and percentage composition of the microflora in biofilms of pig feed, which varies depending on the seasonal factor. Bacteria Streptococcus spp., Pasteurella multocida, Neisseria spp., and Clostridium perfringens in biofilms were found much more often (by 25% or more) in the warm period of the year, while listeria in silage and haylage — in the autumn–winter period. This property of feed biofilms is also significantly influenced by the conditions of cultivation, harvesting and storage of agricultural products. In the study of biofilms of microflora of barley, corn and wheat, it was found that their structural basis are aerobic fungi of the mold Aspergillus spp. Bacteria Streptococcus spp., Pasteurella multocida, Neisseria spp., and Clostridium perfringens without mold form much looser biofilms in vitro and these biofilms are much more sensitive to a wide range of commercial antibiotics. The structural basis of polymicrobial biofilms of barley, corn and wheat microflora is highly likely to be aerobic fungi of Aspergillus spp.
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