The correlation of coronary artery disease (CAD) with pro-oxidant/antioxidant balance and oxidative DNA damage was investigated.Seventy-seven patients with CAD and 44 healthy individuals as control were included in this study. The comparative ratios of ubiquinol-10/ubiquinone-10, 8-hydroxy-2'-deoxyguanosine/deoxyguanosine and the level of MDA measured by HPLC and the activities of GPX and SOD by colorimetric approach in blood samples obtained from patients with CAD were unraveled.8-OHdG/dG ratios, serum MDA level and GPX activity were found significantly elevated level in serum of CAD patients compared to control group. The SOD activity was observed in stable levels in CAD patients. Ubiquinol-10/ubiquinone-10 ratio was significantly lower in patients with CAD than the controls.The positive correlation was observed between 8-OHdG/dG ratios in both MDA levels and GPX activity, while the significant negative correlation was seemed between the ratio of 8-OHdG/dG and ubiquinol-10/ ubiquinone-10 as well as MDA levels and ubiquinol-10/ ubiquinone-10 ratio.We conclude that, both the disruption of pro-oxidant/antioxidant balance and oxidative stress in DNA may play an important role in the pathogenesis of coronary artery disease.

Aim: In the present study, we aimed to assess serum concentrations of zinc (Zn), copper (Cu), iron (Fe), cadmium (Cd), lead (Pb), manganese (Mn), vitamins A (retinol), D (cholecalciferol) and E (α-tocopherol) in patients with coronary artery disease (CAD) and to compare with healthy controls.Methods: A total of 30 CAD patients and 20 healthy subjects were included in this study. Atomic absorption spectrophotometry (UNICAM-929) was used to measure heavy metal and trace element concentrations. Serum α-tocopherol, retinol and cholecalciferol were measured simultaneously by high performance liquid chromatography (HPLC).Results: Demographic and baseline clinical characteristics were not statistically different between the groups. Serum concentrations of retinol (0.3521±0.1319 vs. 0.4313±0.0465 mmol/I, p=0.013), tocopherol (3.8630±1.3117 vs. 6.9124±1.0577 mmol/I, p<0.001), cholecalciferol (0.0209±0.0089 vs. 0.0304±0.0059 mmol/I, p<0.001) and Fe (0.5664±0.2360 vs. 1.0689±0,4452 µg/dI, p<0.001) were significantly lower in CAD patients. In addition, while not statistically significant serum Cu (1.0164±0.2672 vs. 1.1934±0.4164 µg/dI, p=0.073) concentrations were tended to be lower in patients with CAD, whereas serum lead (0.1449±0.0886 vs. 0.1019±0.0644 µg/dI, p=0.069) concentrations tended to be higher.Conclusions: Serum level of trace elements and vitamins may be changed in patients with CAD. In this relatively small study we found that serum levels of retinol, tocopherol, cholecalciferol, iron and copper may be lower whereas serum lead concentrations may be increased in patients with CAD.

Our data have demonstrated that HD patients exhibit increased oxidative DNA damage and decreased antioxidant activity. We propose that endothelial function is negatively correlated with 8-OHdG/dG ratio and positively correlated with antioxidant enzymes. To our knowledge, this is the first study to demonstrate the inverse relationship between endothelial function and plasma oxidative DNA damage in HD patients.

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ÖZETAmaç: Obezite ateroskleroz için önemli bir risk faktörüdür ve sıklığı hızla artmaktadır. Son çalışmalar obezitede ve obezite ile ilişkili komplikasyonlarda leptin ve adiponektinin önemli rol oynadığını düşündürtmektedir. Bununla birlikte etki mekanizmaları tam olarak ortaya konulamamıştır. Bu çalışmada leptin ve adiponektin düzeylerinin vücut kütle indeksi (VKİ) ve oksidatif parametreler ile olan ilişkisi araştırıldı. Yöntemler: Enine-kesitli gözlemsel çalışmaya sağlıklı bireyler arasında VKİ ölçütlerine göre VKİ >35 kg/m 2 olan (n=29, Grup 1), VKİ = 25-30 kg/m 2 arası (n=29, Grup 2) ve VKİ <25 kg/m 2 (n=29, Grup 3) olmak üzere toplam 87 olgu çalışmaya alındı. Leptin ve adiponektin düzeyleri, total antioksidan kapasite (TAK) ve total oksidan seviye (TOS) düzeylerine bakıldı. TAK ve TOS değerleri kullanılarak oksidatif stres indeksi (OSİ) hesaplandı. İstatistiksel analiz Ki-kare, Mann-Whitney U, tek yönlü ANOVA, Kruskal-Wallis, Pearson korelasyon ve çoklu regresyon analiz testleri ile yapıldı. Bulgular: Yaş ve cinsiyet dağılımı gruplar arasında benzerdi. TAK düzeyi grup 1'de en küçük iken grup 3'de en yüksekti. TOS ise grup 1'de grup 2 ve 3'e göre daha yüksek (p <0.05), OSİ grup 2 ve 3'de benzer (p=0.059), grup 1'de diğerlerine göre istatistiksel açıdan anlamlı derecede farklıydı (p<0.05). Leptin düzeylerinde grup 1'den grup 3'e doğru anlamlı azalma, adiponektinde ise artma izlenmekteydi. Leptin düzeyleri TOS (β=-1.123, %95 güven aralığı (GA)=-12.734-0.255, p=0.040), OSI (β=1.689, %95 GA=1.105-12.481, p=0.018) ve bel çevresi (β=-0.592, %95 GA= -0.630-0.134, p=0.003) ile diğer parametrelerden bağımsız olarak ilişki gösterirken, adiponektin düzeyleri herhangi bir parametre ile anlamlı ilişki göstermemekteydi. Sonuç: Sağlıklı kişilerde VKİ artış ile birlikte leptin artmakta, adiponektin ise azalmaktadır. Meydana gelen bu değişikliklerden TOS ve OSI'nin sorumlu olabileceğini düşündürmektedir. (Anadolu Kardiyol Derg 2010; 10: 391-6) Anahtar kelimeler: Adiponektin, antioksidan kapasite, leptin, obezite, oksidatif stres indeksi ABSTRACT Objective: Obesity is an important risk factor of atherosclerosis and its prevalence in humans is increasing. Recent studies suggest that the leptin and adiponectin play important roles in obesity, and they are associated with complications of obesity. However, the mechanism of effects has not been outstandingly established. In this study, we studied leptin and adiponectin levels according to body mass index (BMI) and their relationship with oxidative parameters. Methods: A total of 87 healthy individuals with BMI >35 kg/m 2 (n=29, Group 1), BMI=25-35 kg/m 2 (n=29, Group 2) and BMI <25 kg/m 2 (n=29, Group 3) were included in the cross-sectional observational study. Leptin, adiponectin levels, total antioxidant capacity (TAC) and total oxidant status (TOS) were measured. Oxidative stress index (OSI) was calculated using TAC and TOS values. Statistical analyses were performed using Chi-Square, Mann-Whitney U, one-way ANOVA, Kruskal-Wallis, Pearson correlation and multiple regression an...