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A glycerol auxotroph was isolated from Rhodopseudomonas capsulata for use as a system for studying membrane synthesis and function. When the mutant was deprived of glycerol, net phospholipid synthesis ceased immediately and a small amount of free fatty acids accumulated. A turnover of lipid occurred in both deprived and supplemented cultures. Deoxyribonucleic acid and protein synthesis continued for one doubling of cell mass and then slowed down in deprived cells. Net ribonucleic acid synthesis slowed down more dramatically. Oxidative phosphorylation activity of membrane preparations from aerobically and semianaerobically grown cells appeared unaffected by glycerol deprivation, indicating that simultaneous lipid synthesis is not a requirement for new oxidative phosphorylating activity. In the absence of net phospholipid synthesis, bacterthrough which a mixture of N2 and CO2 was added. Illumination (800 ft-c [ca. 8,608 lx]) was provided by a bank of 60-W tungsten lamps.Strains and mutagenesis. R. capsulata was obtained from G. Sojka. Aerobic cells growing loga-337 on July 31, 2020 by guest

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