Objective: A simple, specific, accurate, and precise method, namely, reverse phase high-performance liquid chromatography was to develop for simultaneous estimation of Lidocaine (LDC) and prilocaine (PLC) in a topical local anesthetic cream.Method: The mixture of PLC and LDC was separated on Hi Q Sil C18 HS column, (250 mm × 4.6 mm, 5 μm), column temperature ambient and flow rate 1.2 mL/minutes. The mobile phase was acetonitrile: 0.01 M diethylamine solution (pH adjusts to 6.8 with orthophosphoric acid) (60:40) with detection at 225 nm.Results: The retention time was found to be 6.075±0.12 minutes for PLC and 8.642±0.15 minutes for LDC, respectively. Linearity was observed in the concentration range of 1-6 μg/mL for both LDC and PLC, respectively. The method was validated according to International Conference on Harmonization guideline and values of linearity, precision, robustness, limit of detection, limit of quantitation, selectivity, and recovery were found to be in good accordance with the prescribed value.Conclusion: The proposed method can be useful in the quality control of LDC and PLC in their topical formulation.
A simple, accurate, sensitive and economical spectrophotometric method have been developed and subsequently validated for determination of Formoterol Fumarate Dihydrate (FFD) and Fluticasone Propionate (FP) in bulk and pharmaceutical formulation. For the simultaneous equation method, the estimation of FFD and FP was carried out at 215nm (λmax of FFD) and 236 nm (λmax of FP), respectively. Calibration curves of FFD and FP were found to be linear in the concentration ranges of 6-36 μg/mL and 20-120 μg/mL for FFD and FP, respectively.
To develop a simple, specific, accurate and precise high performance thin layer chromatography method for simultaneous estimation of Lidocaine and Prilocaine in a topical local anesthetic cream. The mixture of Prilocaine and Lidocaine separated on Aluminum precoated silica gel 60 F254 plates using di-isopropyl ether: methanol: ammonia (10: 0.5: 0.1 % v/v/v) as mobile phase and detection was carried a wavelength of 225 nm The method was validated as per ICH guidelines. The Rf value was found to be 0.45 ± 0.03 for Prilocaine and 0.55 ± 0.02 for Lidocaine, respectively. Linearity was observed in the concentration range of 100-600 ng/spot for both Lidocaine and Prilocaine, respectively. HPTLC method was validated according to ICH guideline and values of linearity, precision, robustness, LOD, LOQ, selectivity, recovery were found to be in good accordance with the prescribed value. Conclusion: The proposed method can be used successfully for routine analysis of Lidocaine and Prilocaine in their topical formulation.
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