Development and Validation of HPLC Method for Simultaneous Determination of Lidocaine and Prilocaine in Topical Formulation

Gowekar, Narendra M; Wadher, S. J.

doi:10.22159/ajpcr.2017.v10i10.15162

Cited by 4 publications

(2 citation statements)

References 20 publications

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“…It was analysed for peaks, tailing factors, theoretical plates and relative retention time. 18 Specificity: Prepared Blank, placebo, standard and sample all had been injected. The blank and placebo did not interfere with the retention time of the Choline salicylate and Lignocaine HCl peak.…”

Section: Methods Validationmentioning

confidence: 99%

RP-HPLC Method Development and Validation of Choline Salicylate and Lignocaine HCl in Mouth Ulcer Gel

Boopalan,

Krishna,

Somasekhar

et al. 2024

Ind. J. Pharm. Edu. Res

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Objectives:The prime intent of the current study is to develop a rapid, reliable, robust and cost-effective reversed-phase HPLC method for simultaneous estimation of Choline Salicylate and Lignocaine HCl in mouth Ulcer Gel. Materials and Methods: Buffer was prepared with 3.5 g of disodium hydrogen orthophosphate in 1000 mL of water and adjusted to pH 4.5 with dilute orthophosphoric acid. A mixture of Buffer and Methanol (55:45) containing 0.01M (2.062 g) 1-Hexane Sulphonic acid comprised the mobile phase. The separation was carried out with a Hypersil C 18 column (250x4.6 mm, 5 µ) maintained at a temperature of 30ºC at a flow rate of 1.0 mL/min with detection at 220 nm. The Injection volume was 20 µL and the run time was 20 min. Results: Retention time for choline salicylate and Lignocaine HCl was 3.163 and 9.629 min respectively. Linearity for Choline Salicylate and Lignocaine Hydrochloride was found to be in the range of 12.5-50 µg/mL. The correlation coefficient for the linear curve obtained between concentration vs. area for standard preparations of Choline Salicylate and Lignocaine HCl was 0.9992 and 0.9993 respectively. The projected procedure successfully satisfied the specificity and robustness parameters. Conclusion: The analytical method was successfully validated according to ICH guidelines (ICH, Q2 (R1)). The proposed novel method is reliable and cost-effective and is well-suited for use in the pharmaceutical industry.

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Section: Methods Validationmentioning

confidence: 99%

RP-HPLC Method Development and Validation of Choline Salicylate and Lignocaine HCl in Mouth Ulcer Gel

Boopalan,

Krishna,

Somasekhar

et al. 2024

Ind. J. Pharm. Edu. Res

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“…A total of 1 g of an optimized NIO-HAMIN emulgel was weighed and extracted using 15 mL methanol with the aid of ultrasonication for 15 min. The solutions were filtered into a 25 mL volumetric flask; Isopropyl alcohol was used to top up to the mark [37]. The prepared sample was analyzed using a developed HPLC, as described in 4.2.3.…”

Section: Drug Uniformity Contentmentioning

confidence: 99%

Formulation and In Vivo Pain Assessment of a Novel Niosomal Lidocaine and Prilocaine in an Emulsion Gel (Emulgel) of Semisolid Palm Oil Base for Topical Drug Delivery

Shabery

Widodo

Chik

2023

Gels

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This study aimed to formulate semisolid niosomal encapsulated lidocaine and prilocaine using the patented palm oil base Hamin-C® for further characterization and in vivo pain assessment. Seven formulations were initially studied with various chemical compositions. A thin-layer film hydration method was used to produce niosome using a mixture of surfactant (Span® 40 or Span® 60) and cholesterol (CHOL) at a 1:1 ratio, with/without a charge-inducing agent (diacetyl phosphate) (DCP) and with/without labrasol®. Niosome F1 formulation had been identified as the highest %EE achieved, at 53.74 and 55.63% for prilocaine and lidocaine, respectively. Furthermore, NIO-HAMIN F1 emulgel indicated the best formulation with higher permeability of prilocaine and lidocaine compared to the rest of the formulations. The reformulation of optimization of NIO-HAMIN F1 emulgel using a cold process to NIO-HAMIN F1-C emulgel to improve the viscosity resulted in higher diffusion of prilocaine and lidocaine by 5.71 and 33.38%, respectively. In vivo pain perception studies by verbal rating score (VRS) and visual analogue score (VAS) on healthy subjects show a comparable local anesthetic effect between NIO-HAMIN F1-C emulgel and EMLA® cream.

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A simple, fast, and cost-effective smartphone-based digital imaging method for quantification of lidocaine hydrochloride in pharmaceutical formulations

Ponce-Rodriguez,

Riera-Williams

2024

Annales Pharmaceutiques Françaises

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Development and Validation of HPLC Method for Simultaneous Determination of Lidocaine and Prilocaine in Topical Formulation

Cited by 4 publications

References 20 publications

RP-HPLC Method Development and Validation of Choline Salicylate and Lignocaine HCl in Mouth Ulcer Gel

RP-HPLC Method Development and Validation of Choline Salicylate and Lignocaine HCl in Mouth Ulcer Gel

Formulation and In Vivo Pain Assessment of a Novel Niosomal Lidocaine and Prilocaine in an Emulsion Gel (Emulgel) of Semisolid Palm Oil Base for Topical Drug Delivery

A simple, fast, and cost-effective smartphone-based digital imaging method for quantification of lidocaine hydrochloride in pharmaceutical formulations

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