Biological characteristics of a subpopulation of cancer stem cells from two triple-negative breast tumour cell lines, HELIYON,
RESUMENLa especificidad entre bacteriófagos y bacterias es una característica utilizada exitosamente para la d de varias especies microbianas. Por este motivo, la detección de vibriófagos es una herramienta útil de in ción y podría ser un método rápido y conveniente de diagnóstico de Vibrio chalerae. El objetivo de este es detectar vibriófagos en muestras de aguas marinas someras y determinar las características morfoló estos vibriófagos.Se determinó la cinética de crecimiento de una cepa de Vfbrfa chalerae serotipo Inaba. Se analizaro tiva y cuantitativamente muestras tomadas de cinco puntos de un sector adyacente a la playa La Chira desembocaduras del río Rímac y río Chillón, usándose distintos inóculos y varios períodos de incubac bacteriófagos fueron concentrados y teñidos para el estudio morfológico por microscopía electrónica de tran Los resultados obtenidos indican que la detección de vibriófagos podría ser una herramienta importan indicador de la presencia de Vibria chalerae.Palabras clav es: Vibria chalerae, vibriófagos , contaminación, aguas, bacteriófagos . ABSTRACTT he specificity between bacteriophages and bacteria is a feature that has been successfully use detection of severaJ microbial species. For this reason th e detection of vibriophages is a valuable research it might be a fast and convenient method to Vibria chalerae diagnostics . The goal of this study was vibriophages in shallow seawater samples and to determine the morphological characteristics of these vibrio It was determined the growth kinetics of a strain of Vibria chalerae serotype Inaba. Seawater sampl from five shallow points from an adjacent sector to La Chira beach, and Rimac and Chillon river mou analysed qualitatively and quantitatively using different inoculum volumes and several incubation peri bacteriophages were concentrated and stained for the morphological study by transmission electron micThe results we obtained indicate that the vibriophages could be an important tool as a Vibria cholerae
Hepatic fibrosis is caused by chronic injury due to toxic, infectious, or metabolic causes, and it may progress to cirrhosis and hepatocellular carcinoma. There is currently no antifibrotic therapy authorized for human use; however, there are promising studies using cell therapies. There are also no animal models that exactly reproduce human liver fibrosis that can be used to better understand the mechanisms of its regression and identify new targets for treatment and therapeutic approaches. On the other hand, mesenchymal stem cells (MSC) have experimentally demonstrated fibrosis regression effects, but it is necessary to have an animal model of advanced liver fibrosis to evaluate the effect of these cells. The aim of this work was to establish a protocol for the induction of advanced liver fibrosis in rats using thioacetamide (TAA), which will allow us to perform trials using MSC as a possible therapy for fibrosis regression. For this purpose, we selected 24 female rats and grouped them into three experimental groups: the control group (G-I) without treatment and groups II (G-II) and III (G-III) that received TAA by intraperitoneal injection for 24 weeks. Then, 1 × 106 / kg adipose mesenchymal stem cells (ASCs) were infused intravenously. Groups G-I and G-II were sacrificed 7 days after the last dose of ASC, and G-III was sacrificed 8 weeks after the last ASC infusion, all with xylazine/ketamine (40 mg/kg). The protocol used in this work established a model of advanced hepatic fibrosis as corroborated by METAVIR tests of the histological lesions; by the high levels of the markers α-SMA, CD68, and collagen type I; by functional alterations due to elevated markers of the hepatic lesions; and by alterations of the leukocytes, lymphocytes, and platelets. Finally, transplanted cells in the fibrous liver were detected. We conclude that TAA applied using the protocol introduced in this study induces a good model of advanced liver fibrosis in rats.
Background and Aim: Ribonucleic acid viruses remain latent in different cell types, including mesenchymal stem cells; however, the distemper virus remains undetected in these cells. This study aimed to determine whether adipose stem cells (ASCs) from dogs with distemper disease are infected with the canine morbillivirus (CM). Materials and Methods: Twelve dogs with the neurological phase of the disease and who were positive for CM by reverse transcription polymerase chain reaction (RT-PCR), were studied. ASCs from adipose tissue of the lesser omentum of these infected dogs were isolated and characterized. Direct fluorescence was used to detect the viral antigen in cell cultures. Flow cytometry and RT-PCR identified detectable quantities of the virus in two cultures, while electron microscopy confirmed the CM particles within ASCs. Results: This study revealed that ASCs of the omentum of dogs with distemper disease can be infected with CM, indicating their possible involvement in this virus latency and persistence. This suggests that its detection should be considered within the quality control process of stem cells intended for regenerative medicine. Conclusion: To the best of our knowledge, this is the first study that demonstrates that omentum ASCs from dogs with distemper disease can be infected with CM and may be involved in viral latency or persistence. Our study also suggests that the detection of CM should be considered within the quality control process of stem cells intended for regenerative medicine.
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