No abstract

No abstract

Background: Burns are among the most prevalent injuries in humans with high cost in health care and heavy prolonged or permanent physical, psychological and social consequences. Commercial antimicrobial creams and dressing agents are unsuccessful in healing deep burn wounds. Materials and Methods: A study was conducted to assess the impact of crude linseed oil (LSO) topical application on burn wounds healing in rabbits in comparison with untreated wounds (NAT) and those treated with Vaseline gel (VAG) and Cicatryl-Bio ointment (CBO). By the 28 th day post burning, skin biopsies were analyzed for histological and cytological lesions. The presence of various bioactive phytochemical groups in linseed was also screened. Results: Phytochemical screening has resulted in high concentrations of flavonoids and terpenoids, low amounts of catechic tannins and total absence of alkaloids and saponosides. All along the trial, the rate of wounds contraction was found to be significantly higher in burns treated with LSO which had also a significant shorter healing period (26±5.89 days) as compared to the other treatments. LSO healed wounds included less inflammatory cells, complete epithelium regeneration with a reduced thickness of the new formed dermis, discreet fibrosis, enhanced neo-vascularization, increased number of collagen fibers, fibroblasts and many myofibroblasts. Additionally, no adverse effects of LSO on cicatrization process were recorded. Conclusion: These findings prove the safety and efficaciousness of linseed oil topical application in the therapy of burn wounds.

The essential oil of Matricaria pubescens (Asteraceae) collected at Ghardaia (Algerian Septentrional Sahara) was studied by GC and GC-MS. Isochrysanthemic acid ethyl ester (26.5%), spathulenol (19.4%), α-cadinol (12.9%) and geranylisovalerate (8.2%), were identified as the major components of the essential oil, which was investigated for its analgesic effect.

and B. Ait-Kaki 2Pituranthos scoparius (Coss. & Dur.) Schinz (Umbelliferae) is a saharian species [1] used in traditional medicine for the treatment of asthma and rheumatism [2][3], Touareg people also use it in food as aroma for meal and bread [3]. The GC and GC/MS analysis of the essential oils of Pituranthos scoparius stems and seeds, collected from Ghardaia [4] (septentrional Sahara) during spring, allowed us to identify the respective components with relative percentages which appeared to be different from those reported from the species collected during winter at Ain Diss [5] (presaharian). The comparative percentages of the major components (more than 3%) of the stem and seed oils for each specimen (saharian and presaharian) are given in Table1.The species Pituranthos scoparius has not been the subject of biological studies. We report here the comparative antibacterial activities of the essential oils of stems and seeds of the species collected at Ghardaia, using the disk diffusion method. The MIC (minimum inhibitory concentration) values are given in Table 2-3. Both of the essential oils inhibited the growth of the tested strains, but the stems oils inhibited remarkably the growth of the microorganisms namely Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis, and Klebsiella pneumoniae at the respective concentation levels 1mg/ml, 2 mg/ml, and 16mg/ml with 32 mm, 30 mm, and 28 mm inhibition zone diameters while the seed essential oil showed lowest MIC values with Proteus mirabilis, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC25923 at 0.156 mg/ml, 1.25 mg/ml, and 20 mg/ml with 30 mm, 28 mm, and 28 mm inhibition zone diameters, respectively.

From the n-BuOH extract of the aerial parts of Genista ulicina, six triterpene saponins, 3-O-β-D-glucopyranosyl-olean-12-ene-3β,27,28,30-tetraol, 3-O-β-D-glucopyranosyl-olean-12-ene-3β,27,28,29-tetraol, 3,29-di-O-β-D-glucopyranosyl-olean-12-ene-3β,27,28,29-tetraol, 3-O-β-D-glucopyranosyl-olean-12-ene-3β,28,29-triol-27-oic acid, 3-O-β-D-glucopyranosyl-olean-12-ene-3β,27,28-triol-29-oic acid, and 3-O-β-D-glucopyranosyl-14-H-27-nor-olean-12-ene-3β,28,29-triol, were isolated together with eight known triterpene saponins and six flavonoids. Their structures were established mainly by means of spectroscopic methods (1D and 2D-NMR as well as HR-ESI-MS). The n-BuOH extract, investigated for its antitumor growth inhibition of human colon cancer HT-29 cells, presented no significant activity (IC50>100 μg).

HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

A new triterpenic diester, 3,21-dipalmitoyloxy-16β,21α-dihydroxy-β-amyrine(1), along with two natural cyclitols, conduritol C (2) and viburnitol (3), four known triterpenes (4-7), and seven known flavonoids (8-14) were isolated from the aerial parts of Chrysanthemum macrocarpum. Their structures were established on the basis of extensive 1D and 2D NMR ( 1 H, 13 C, COSY, HMBC, HSQC, and ROESY) and ESIMS studies. The chloroform fraction, taraxasterol (4) and β-sitosterol ( 7) were investigated for their antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae. The chloroform fraction and taraxasterol (4) showed a weak antibacterial activity and were evaluated for their cytotoxic activity against human colon cancer HT-29 cells and human prostate carcinoma PC3 cells. The results indicated that both the chloroform fraction and taraxasterol (4) inhibited cell proliferation of both PC3 and HT-29 cells.