EVALUATION OF CRUDE FLAXSEED (Linum usitatissimum L.) OIL IN BURN WOUND HEALING IN NEW ZEALAND RABBITS

Abstract

Background: Burns are among the most prevalent injuries in humans with high cost in health care and heavy prolonged or permanent physical, psychological and social consequences. Commercial antimicrobial creams and dressing agents are unsuccessful in healing deep burn wounds. Materials and Methods: A study was conducted to assess the impact of crude linseed oil (LSO) topical application on burn wounds healing in rabbits in comparison with untreated wounds (NAT) and those treated with Vaseline gel (VAG) and Cicatryl-Bio ointment (CBO). By the 28 th day post burning, skin biopsies were analyzed for histological and cytological lesions. The presence of various bioactive phytochemical groups in linseed was also screened. Results: Phytochemical screening has resulted in high concentrations of flavonoids and terpenoids, low amounts of catechic tannins and total absence of alkaloids and saponosides. All along the trial, the rate of wounds contraction was found to be significantly higher in burns treated with LSO which had also a significant shorter healing period (26±5.89 days) as compared to the other treatments. LSO healed wounds included less inflammatory cells, complete epithelium regeneration with a reduced thickness of the new formed dermis, discreet fibrosis, enhanced neo-vascularization, increased number of collagen fibers, fibroblasts and many myofibroblasts. Additionally, no adverse effects of LSO on cicatrization process were recorded. Conclusion: These findings prove the safety and efficaciousness of linseed oil topical application in the therapy of burn wounds.

Vegetable butters and oils in skin wound healing: Scientific evidence for new opportunities in dermatology

“…Throughout the study, burns treated with linseed oil were found to be healing faster and showed significantly higher wound contraction than the control treatments. Less inflammatory cells, complete epithelial regeneration with a reduced thickness of the newly formed dermis, fibrosis, enhanced neovascularisation, and an increased number of collagen fibres, fibroblasts, and myofibroblasts were observed in wounds treated with linseed oil (Beroual et al, ).…”

Evaluation of the wound healing activity of twelve herbal and non-herbal remedies used in Sana'a-Yemen for the treatment of wounds and burns

“…• Fixedoil [139] • α-linolenicacid [139,140] • Tocopherol(vitaminE) [141] •Flaxseedoil [142] •Flaxseedhulloil [143] • Pteroleumetherseedsextract containing palmitic acid, linoliec acid and oleic acid [144] •Fixedoil [145] • Fixedoil [117,141,[146][147][148] • α-Linolenic acid-rich linseedoil [149] • Oleicacid [150] • linoleicacid [146,150] • α-Linolenic acid [146,149,150]…”

“…L. usitatissimum seeds fixed oil was found to inhibit the prostaglandin 2-, leukotriene-, histamine-, bradykinin-, and arachidonic acid-induced inflammation [139]. L. usitatissimum seeds constituents, α-linolenic acid and tocopherol, have been shown to inhibit COX and LOX pathways [140] and to attenuate proinflammatory cytokines and chemokines production [141], respectively. M. communis leaves essential oil, topically applied, was found to inhibit the migration of neutrophils to the inflamed area and the TNF-α and IL-6 formation [155].…”

“…by promoting the proliferation of peripheral blood B-and T-lymphocytes in cell culture, activating phagocytes from blood, stimulating monocytes in cell culture to release the cytokines TNF-α, IL-1 and IL-6, and augmenting the immune response by supplying glucose that is essential for the 'respiratory burst' in macrophages, as well as providing substrates for glycolysis, the major mechanism of energy production in the macrophages [130,131]. L. usitatissimum seeds oil has been shown to exhibit wound healing by several mechanisms of action including cicatrizing and emollient effects, less inflammatory cells in the period of re-epithelization, shortening the inflammatory stage, completing epithelium regeneration, discreet fibrosis, enhancing neo-vascularization, increasing number of collagen fibers, fibroblasts and many myofibroblasts, improving migration of fibroblasts, higher wounds contraction and shortening the healing period [117,141,146,148,149]. A topical administration of a semisolid formulation of linseed oil (SSFLO) (1% or 5%) composed of commercial linseed oil in petroleum jelly was found to promote a significant (P < .05) complete re-epithelialization of skin wounds in 100% of the animals treated compared to negative control (petroleum jelly) at the end of the experiment (14 days), but the same was not observed in groups treated with 10% SSFLO and linseed oil.…”

Herbal Therapy for Burns and Burn Scars

“…Ficus asperifolia, Bridelia ferruginea, Gossypium arboreum [3], Cucurbita moschata [50], Linum usitatissimum L. [51], Sesamum indicum L., Pistacia atlantica Desf., Cannabis sativa L., Juglans regia L. [52], Scutellariae (altissimae, galericulatae, hastifoliae) [53].…”