This study aimed to measure oxidative stress parameters during pig slaughter preparation. This study used 60 landrace pigs that were taken the serum and saliva samples under conditions before stunned (T1), after stunned (T2) and after slaughtered (T3). Superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GPx) were also evaluated. The serum was taken through the jugular vein with vacutainer. Saliva was taken with a salivate tube. Serum and saliva were tested using the Cortisol ELISA immunoassay method. The level of SOD, MDA and GPx were analyzed through serum concentration. The results showed significant differences (p<0.05) in T2 and increased significantly (p<0.05) in T3. The SOD levels showed the opposite results with MDA and GPx. The SOD levels decrease after stunning and increase after slaughter, vice versa. It can be concluded that there were significant differences in serum and salivary cortisol of pigs taken before stunned, after stunned and after slaughtered. The level of SOD, MDA and GPx could be a reference for oxidative stress parameters during handling and restraint in pig slaughter.
Laporan kasus ini bertujuan untuk menjelaskan mengenai kasus otitis eksterna dan auricular hematoma (othematoma) yang menyerang anjing Samoyed berusia 8 tahun. Diagnosis ditetapkan berdasarkan informasi yang diperoleh dari anamnesis pemilik anjing serta hasil dari pemeriksaan klinik. Prosedur tata laksana yang paling sering digunakan untuk menangani kasus ini adalah melalui tindakan operasi, serta terapi sistemik pada perawatan pascaoperasi menggunakan kombinasi dari obat antiinflamasi, antibiotik, koagulan dan antihistamin. Berdasarkan pernyataan dari pemilik anjing Samoyed, penilaian penanganan yang telah dilakukan memberikan hasil baik dibuktikan melalui waktu penyembuhan otitis eksterna dan auricular hematoma anjing berkisar 2 minggu.
Timor deer (Cervus timorensis) at Surabaya zoo, Indonesia, that were found to be naturally infected with Fasciola, showed elevated level of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP). Of a total of 75 deer examined, 12 (25%) of the 47 adult deer and 8 (29%) of the 28 juvenile deer were found to be infected with fascioliasis, as evidenced by the shedding of the parasite eggs. The level of ALT, AST and ALP were significantly elevated (p<0.05) in all the infected deer. Only Fasciolainfected deer showed elevated serum liver enzyme. Deer with elevated enzyme level show a trend that positively correspond with higher Egg per gram of feces (EPG). The average size of the parasite eggs at 169.0±11.1 × 96.0±3.5μm, correspond well with that of Fasciola gigantica. No other trematode eggs were observed besides that of F. gigantica. There was no significant difference in the enzyme profile between the two sexes in both the infected and the uninfected group. This is the first report of the elevation of serum liver enzyme in Timor deer that is associated with not only fascioliasis and also correspond positively with the EPG.
This study aimed to determine the antiproliferation effect of Glycine max Linn ethanolic extract on Sprague Dawley albino rats induced by 7,12-dimethylbenz(á)anthracene (DMBA) on the mammary gland. This study used 20 albino rats then divided into five treatment groups, i.e., positive control (C+) induced DMBA; negative control (C-) none treatment; Treatment groups (T1; T2; T3) induced DMBA then followed extract dose (5; 10; 20 mg/day). DMBA induction was given every two days for 20 days subcutaneously. Administration of Glycine max Linn extract was given peroral for 14 days. It can be concluded that Glycine max Linn ethanolic extract inhibits the proliferation cell in mammary gland carcinoma in albino rats revealed on aspect hyperchromatization, tubular formation, and shape of the nuclei.
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