The objective of this research was to examine the effect of removal raffinose in tris aminomethane egg yolk diluents to semen quality of Boer goat during chilled preservation. This research was carried out at Animal Reproduction Laboratory and Sumber Sekar Laboratory, Animal Husbandry Faculty, Brawijaya University from December 11 th 2016 to January 31 th 2017. Semen diluent was divided into two groups, there were P0 (80% Tris Aminomethane + 20% Egg Yolk); and P1 (80% Tris Aminomethane (without raffinose) + 20% Egg Yolk). Parameter of this research was motility percentage, viability percentage, and abnormality percentage. Data of this research was analyzed using paired design t test. The result showed that after five days chilled preservation, was different (P<0,05) on the average motility percentage between P0 (39,50±9,26%) and P1 (43,50±6,26%). There was significantly different (P<0,01) on the average viability percentage between P0 (45,86±15,66%) and P1 (46,38±15,32%). There was significantly different (P<0,01) on the average abnormality percentage between P0 (1,76±0,60%) and P1 (2,31±0,75%). Total motile sperm count after 5 days chilled preservation was different (P<0,05) in P0 treatment and not different (P>0,05) in P1 treatment compared to the expectation value of 40 million sperm/ml. The conclution of this research was the tris aminomethane egg yolk diluent without raffinose could protect of Boer goat semen quality during chilled preservation.

The experiment was conducted from November 2011 to January 2012 in BBIB Singosari.The study aims was to determine the effect of thawing techniques on the quality frozen bovine sperm Bali, Madura and PO cattle. This material used 90 frozen straw of three cattle from the three breed. The method is an experiment with three treatments and 10 replications for frozen to thawing in temperature wateris 5 0 Cof duration 60 seconds, 15 0 C duration 30 seconds and 37 0 C duration15 seconds. Variables observed in this study were sperm quality are motility, viability and abnormality. Data in percentage transformed in to arcus sinus were analyzed using Completely Randomized Block Design (CRBD) with replication as blocks of 10x.The results showedthe thawing at 37 0 C duration 15 seconds to highly significant effect (P<0,01) viability and motility and significant effect (P<0.05). The abnormalities, no significant effect (P>0,05). Breed cattle not significant effect to quality.It can be cocluded that thawing techniques impact the motility and viability in frozen bovine semen spermatozoa Bali, Madura and PO cattle. Thawing using 37 0 C temperature water with duration of 15 seconds is the best method to obtain the quality of spermatozoa post thawing frozen sperm.Key words : thawing tecnique, sperm quality. Sapi Bali, Madura dan PO merupakan sapi lokal sebagai potensi peternakan nasional perlu diberdayakan sehingga berkontribusi dalam penyediaan daging nasional. Upaya perbaikan mutu genetik ternak sapi Bali, Madura dan PO sebagai potensi sumber daya lokal di sektor peternakan perlu dilakukan dengan tujuan untuk perbaikan kualitas produksi dan kelestarian genetik Salah satu cara ke arah itu adalah melalui introduksi tehnologi IB dengan semen beku ke tiga bangsa sapi tersebut. Namun seringkali terjadi gagal kebuntingan disebabkan rendahnya kualitas semen beku post thawing. Indikator rendahnya kualitas semen beku post thawing antara lain rendahnya motilitas massa ataupun individu, rendahnya angka viabilitas dan tingginya angka abnormalitas. Hal ini disebabkan salah satunya handling semen beku seperti thawing.Thawing dimaksudkan untuk mencairkan kembali semen beku dengan menggunakan media dan durasi tertentuCorresponding author : adesalim@yahoo.com sehingga dapat dideposisikan ke alat reproduksi betina. Kondisi ini menimbulkan heat shock effect maupun kontaminasi dengan oksigen pada spermatozoa sehingga mempengaruhi kestabilan membran yang berdampak pada kualitas semen beku. Rodriguez, et al., (2005), melaporkan bahwa proses thawing pada semen beku sapi dengan suhu 37 o C selama 60 detik menyebabkan terjadinya beberapa kerusakan pada membran spermatozoa. Sedangkan Ansari et al., (2010) melaporkan motilitas, viabilitas dan integritas membran tertingi yaitu thawing pada air bersuhu 37 o C selama 30 detik. Pesch and Hoffmann (2007), menyarankan untuk keperluan IB komersil pada sapi, sebaiknya thawing dilakukan pada air bersuhu 37 o C selama 20 detik karena lebih praktis serta semen beku tidak boleh dithawing di bawah suhu 15 o ...

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ABSTRAK. Keberhasilan IB kambing dipengaruhi salah satunya kualitas pejantan yang ditandai kemampuan menghasilkan semen dengan kualitas makroskopis dan mikroskopis tinggi. Kemampuan ini dipengaruhi oleh sexual behaviornya. Sebagai daerah transmigrasi yang berpotensi untuk pegembangan peternakan kambing, sekaligus banyak populasi kambing kacang di Halmahera Timur, sehingga desa Bina Gara menjadi lokasi sampel penelitian ini. Penelitian ini dilakukan dengan tujuan untuk mengevaluasi sexual behavior sebagai gambaran kinerja reproduksi kambing Kacang jantan. Materi yang digunakan yaitu 9 pejantan dan 5 betina yang dibagi dalam 3 kelompok umur masing-masing P1 (1,5 tahun), P2 (2,5 tahun) P3 (4 tahun). Metode yang digunakan yaitu survey dengan pemilihan sampel secara purposive sampling dengan variabel yaitu waktu reaksi, jumlah fals mounting, daya jepit, daya dorong dan kualitas ereksi. Data dianalisis deskriptif menggunakan software genstat 14.2. Hasil yang diperoleh yaitu waktu reaksi P2 terbaik = 1,07±0,13, fals mounting rata-rata 2x di semua kelompok umur, daya jepit dan daya dorong 3+ di semua kelompok umur dan skor kualitas ereksi 3+ pada semua kelompok umur. Kesimpulan penelitian ini yaitu tingkah laku sexual kambing kacang jantan di Unit Pemukiman Transmigrasi (UPT) Desa Bina Gara Kabupaten Halmahera Timur secara umum memiliki performans terbaik pada semua kelompok umur, ditandai score false mounting 2 kali, daya jepit +3 dan kualitas ereksi +3, sedangkan untuk waktu reaksi skor tertinggi yaitu pada kelompok umur 2,5 tahun. (The evaluation of sexual behavior on kacang buck (capra hircus) in transmigration settlement unit (TSU) Bina Gara Village East Halmahera District) ABSTRACT. The success of Artificial Insemination goat is related to quality service quality with high macroscopic and microscopic qualities. This ability is subject to sexual behavior. As a transmigration area that shifts for the development of goat farms, as well as many Kacang Buck populations in East Halmahera, so the village of Bina Gara became the sample location of this study. This study was conducted with the aim to raise sexual behavior as a description of the performance of Kacang Buck. The material used is 9 males and 5 females divided into 3 age groups of each P1 (1.5 years), P2 (2.5 years) P3 (4 years). The method used is survey with sample selection by purposive sampling with reaction time variabel, the amount of fals mounting, clamp power, thrust and erection quality. The data were analyzed descriptively using software genstat 14.2. The result is the best reaction time P2 = 1.07 ± 0.13, False mounting averages 2x in all age groups; 3-clamp force and thrust in all age groups and 3+ erectile quality score in all age groups. This research is sexual behavior of goat beans in Transmigration Resettlement Unit (TRU) of Bina Gara Village of East Halmahera Regency has the best performance in all age group, cleanliness 2 times, power +3 clamp and erection quality +3, while for time The highest score reactions are in the 2.5 year age group.

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ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety