Penelitian tentang pertumbuhan udang vaname (Litopenaeus vannamei) yang dikultur pada sistem bioflok dengan penambahan probiotik telah dilakukan selama 40 hari di Laboratorium unit produksi, Fakultas Perikanan dan Ilmu Kelautan Universitas Halu Oleo Kendari. Penelitian bertujuan untuk menentukan dosis probiotik yang tepat, dan mampu meningkatkan pertumbuhan udang vaname pada budidaya sistem bioflok. Penelitian didesain dengan menggunakan Rancangan acak lengkap dengan 5 perlakuan dan 3 ulangan. Perlakuan yang diterapkan adalah A (tanpa bioflok), B (bioflok), C (bioflok + probiotik 108CFU/mL), D (bioflok + probiotik 1010CFU/mL), dan E (bioflok + probiotik 1012CFU/mL). Wadah yang digunakan adalah akuarium berukuran 35x35x40 cm, dilengkapi aerasi. Hewan uji adalah juvenil udang vaname berukuran 3 – 4 g, yang dipelihara dengan kepadatan 20 ekor/akuarium. Selama pemeliharaan udang diberi pakan sebanyak 5% dari biomassa udang. Penambahan molase dilakukan setiap pagi ke media bioflok sebanyak 4 g. Hasil penelitian menujukkan bahwa perlakuan memberikan pengaruh yang berbeda nyata terhadap kelangsungan hidup, pertumbuhan mutlak rata-rata, laju pertumbuhan spesifik, efisiensi pakan, rasio konversi pakan, dan retensi protein, namun tidak memberikan pengaruh yang berbeda nyata terhadap volume flok. Secara umum terlihat bahwa perlakuan terbaik didapatkan pada penggunaan bioflok dengan penambahan probiotik 1010CFU/mL. Kata kunci: Pertumbuhan, udang vaname, Litopenaeus vannamei, bioflok, probiotik

This study was conducted to determine the effects of inorganic selenium (sodium selenite) and organic selenium (selenomethionine) supplementation on growth and viability of juvenile humpback grouper (Cromileptes altivelis). The experiment was designed as a completely randomized design with eight treatments and three replications. The treatment being tested was source and dosages of selenium ie., inorganic selenium (sodium selenite) supplementation with dosages of 0.5, 1, 2, and 4 mg Se/kg diet and organic selenium (selenomethionine) with dosages of 1, 2, and 4 mg Se/kg diet. Another treatment was unsupplemented selenium. Juveniles humpback grouper at an initial average length of 6,39±0,41 cm and body weight of 4,49±0,65 g were reared in 90×40×35 cm 3 aquaria and fed artificial diet (pellet) two times daily (08.00 and 16.00) at satiation. Fishes were reared for 40 days at a stocking density of 12 fish per aquarium on sea water with salinity of 30-31 ppt and temperature of 28-29 °C. The results of this study showed that the source of selenium supplementation (inorganic or organic) affected growth performance, glutathione peroxidase (GPx) enzyme activity, and blood profiles of the experimental fish. Generally, it was found that selenomethionine supplementation resulted in better fish performance than sodium selenite. In sodium selenite supplementation, survival declined with the increased dosages of Se in the diet, and the supplementation at dosage of 0,5 mg Se/kg diet showed a toxic effects. Histopatological test showed that there were damage in livers, kidneys, and intestines of fish supplemented with sodium selenite from 0.5 to 4 mg Se/kg diet. On the contrary, supplementation of selenomethionine up to 4 mg Se/kg did not show any signs of toxicity and the survival was on 86.11 to 97.22%. Feed efficiency, protein retention, lipid retention, and Se retention indicated that a dosage of 4 mg Se/kg selenomethionine supplementation was the best dosage.Keywords: selenium, viability, growth, Cromileptes altivelis, humpback grouper, ABSTRAKPenelitian ini bertujuan untuk menentukan dosis penambahan selenium anorganik (sodium selenite) dan organik (selenometionin) yang mampu meningkatkan pertumbuhan dan daya tahan tubuh juvenil ikan kerapu bebek (Cromileptes altivelis). Percobaan didesain menggunakan rancangan acak lengkap dengan delapan perlakuan dan tiga ulangan. Perlakuan yang diujikan adalah jenis dan dosis penambahan selenium, yaitu selenium anorganik dengan dosis 0,5; 1; 2; dan 4 mg Se/kg pakan dan selenium organik dengan dosis 1, 2, dan 4 mg Se/kg pakan. Perlakuan lain adalah tanpa penambahan selenium. Juvenil ikan kerapu bebek berukuran panjang awal rerata 6,39±0,41 cm dan bobot tubuh 4,49±0,65 g dipelihara pada akuarium berukuran 90×40×35 cm 3 dan diberi pakan buatan berbentuk pelet secara at satiation dengan frekuensi dua kali sehari (pukul 08.00 dan 16.00). Ikan dipelihara selama 40 hari dengan padat penebaran 12 ekor per akuarium pada air laut bersalinitas 30-31 ppt dan suhu 28-29 °C. Hasil penelitian m...

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This study aimed to determine the optimum dose of apple snail to improve the growth and albumin content of snake head fish. The fish were reared in 9 plastic container (diameter of 50 cm and height 55 cm). All container were filled with freshwater 10 L in each container filled with 5 fish (initial weight : 32,3 ± 1,7g). Three doses of apple snail : 10% of fish biomassa (treatment A), 15% of fish biomassa (treatment B) and 20% of fish biomassa (treatment C) fed to the fish for 45 days of rearing. The fish fed with apple snail in three times a day (08.00, 12.00 and 16.00). The results showed that the fish fed with different dosage of apple snail were not significantly different in weight gain, spesific growth rate (SGR), feed conversion ratio (FCR) feed eficiency (FE) and survival rate (SR) of snake head fish. The weight gain of fish was ranged between 19.55 ± 1.78-25.60 ± 2.10 g, SGR of fish was ranged between 1.05 - 2.23%, FCR of fish was ranged 4.3 - 6.19 , FE of fish was ranged between 16.58 - 23.30%. The highest of feed consumption was found fish fed with 15% and 20% of apple snail.  While, the highest of albumin content was found in the fish fed with 15% of apple snail dosage.Keywords : growth, albumin, snake head fish, Channa striata, apple snail

This study aims to determine the optimum dose of mangrove leaf extract (A. marina) which is able to improve the resistance of vaname shrimp (L. vannamei). The stages in this study consisted of : (1) extraction of mangrove leaves and, (2) Immunostimulation of shrimp with extracts orally, (3) analysis of shrimp resistance to defend against Vibrio alginolitycus bacterial infection through challange test. The treatment was conducted in different doses of extract in feed (10, 15, 20 g/kg), and the control treatment was pellets without extract. Feeding with the addition of the extract was carried out for 40 days, then tested against the bacterium V.alginolitycus. The parameter observed in this study was Survival Rate (SR) and clinical signs. In the treatment of 20 g showed an optimal increase in the vaname shrimp immune response with significantly different result compared to other treatments. Keywords : Vannamei,  Mangrove  Leaves,  V. alginolyticus,  Immunity