In this study, the effects of spermiation time are investigated on milt quality of Russian sturgeon over the course of the spawning season. The milt samples were collected from three broodstock batches at three time points including: the beginning, middle and at the end of the spawning season. According to the results, the milt quality parameters including pH, sperm density, spermatocrit, duration of sperm motility and percentage of sperm motility were significantly low in the beginning and end of season than middle of season. The values of milt quality parameters in the middle of season were as follows: (motility percentage: 69.6 AE 3.5, motility duration: 460.3 AE 37.2 s, sperm density: 8.7 AE 0.4 9 10 9 , milt volume: 86.3 AE 8.1 and milt pH: 8.3 AE 0.15). Significant positive correlations were also found between milt pH and sperm motility as well as between sperm density and spermatocrit. In conclusion, our study showed that the middle of season is the best time for collection of milt with appropriate quality in Russian sturgeon. Selection of milt with good quality is necessary aim to cryopreservation of spermatozoa in endangered fish species including Russian sturgeon.
(2015) Changes of seminal fluid sex steroids and milt quality indices during sequential striping of male Russian sturgeon, Acipensergueldenstaedtii, Brandt and Ratzeburg (1833) The present study was conducted to understand changes of seminal fluid sex steroids and milt quality of Russian sturgeon during sequential stripping. Milt samples were collected three times with 24 intervals. According to results, the values of sperm motility duration, sperm production and seminal fluid steroids including 17a-Hydroxyprogesterone (OHP), testosterone (T) and estradiol-17β (E2) decreased significantly during sequential stripping. The levels of OHP showed a close positive correlation with percentage and duration of sperm motility. Similar correlation was found between T and E2 with sperm concentration. The P concentrations did not have significant correlation with sperm quality parameters. Our results revealed that the ability of Russian sturgeon in production of milt with desirable quality decreased with repetition of milt collection. Changes of seminal fluid steroids may be an image of their serum changes during spermiation period. As well as, seminal fluid sex steroids may affect milt quality after spermiation.
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