This study aimed to evaluate the anti-inflammatory effect of TQ-PLGA/PVA in treatment of oral carcinogenesis. Nanoparticles were fabricated via emulsification-solvent technique. Their characterization including the morphological analysis and size distribution on NPs performed using TEM and the structure composition was carried out via the FTIR. The charge determined via the DLS and ZP analysis. This study was carried out on 24 hamsters and divided into: group A served as negative control, group B was painted with DMBA for 6 weeks then subdivided into three subgroups: B 1 served as positive control, B 2 was injected with TQ, B 3 was injected with TQ-PLGA. Assessment of the blood cells counts, histologically, immunohistochemically through detection of TNF-α protein were performed. Comparisons between groups were performed using ANOVA. The results showed proper encapsulation for TQ-PLGA, significant regression in tumors histologically, marked improvement in blood cells and expression levels of TNF-α protein. Conclusion: systemic application of TQ-PLGA was considered a promising chemo-therapeutic agent in tumors regression.
Introduction: Oral squamous cell carcinoma (OSCC) shows unsatisfied survival rate which remains not changed over the last years. Thymoquinone (TQ) is a phytochemical, and many studies proved its promising therapeutic effect against OSCC. Gold nanorod (GNR) is a photothermal agent, has an efficient near-infrared heat conversion and induces ablation of solid tumors. The combination of photothermal therapy (PTT) and chemotherapy can enhance synergistic effects that markedly exceed the sum of individual treatments alone. Aim: this study aimed to evaluate the effect of combined photothermal and nano chemotherapy in treatment of induced oral squamous cell carcinoma in hamsters.
Material and Methods:In the present study, the combined drug GNR/NTQ loaded on (poly) (lactic-co-glycolic acid) (PLGA) was prepared. The targeting agent, folate or folic acid (FA), was conjugated to the prepared nanoparticles. The therapeutic activity of the drug against chemically-induced OSCC in hamster buccal pouches was evaluated by clinical observations, tumor volume analysis, histopathological alterations and blood analysis. Results: The combined drug (GNR/NTQ/PLGA/FA/laser) had the highest therapeutic activity compared to individual treatments. It significantly ablated
Objectives: Combining the anti-tumor effect of thymoquinone (TQ) with the efficient penetration of gold nanoparticles (GNPs) into cells and nuclei as an attempt to treat the induced oral squamous cell carcinoma in buccal pouch of hamster. Materials and methods: This study was carried out on forty two male Syrian golden hamsters (n=42), its age ranged from 6 to 7 weeks, weighting 90-110 gm. Animals were housed with controlled temperature and were given pellets formed of seeds, grain, cracked corn and tap water ad libitum. The hamsters were divided into: Group A: (Control groups) n=24, Group A1: eighteen animals (n=18) were served as "negative control". Subgroup A1a: Six animals were sacrificed at day zero. Subgroup A1b: Six animals were sacrificed at 14th week. Subgroup A1c: Six animals were sacrificed at 20th week. Group A2: Six animals (n=6) were considered as "positive control". DMBA was painted to the left cheek pouches, three times /week for 14weeks. Group B: Eighteen animals (n=18) were painted with DMBA to left cheek pouches three times/week for 14weeks, and subdivided into: Subgroup B1: Six animals were injected intra-peritoneal with GNPs/3 times/week for six weeks. Subgroup B2: Six animals were injected intra-peritoneal with TQ/3 times/week for six weeks.Sub-group B3: Six animals were injected intra-peritoneal with GNPs-TQ/3 times/ week for six weeks. All pouches were extracted and prepared to examined through histological examination for any structural changes and immunohistological detection of COX2. Results: Improve superior anti-inflammatory role of TQ when loaded with GNPS in COX-2 retardation and tumor size regression. Conclusion: Thymoquinon loaded on nanogold particle showed protective role in the oral cancer.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.