Objective: Iron deficiency (ID) is a frequent complication in end stage renal insufficiency. These patients have to be diagnosed and treated to reduce the prevalence of anemia. Functional iron deficiency (FID) is a situation that can disrupt biochemical iron tests and mask an eventual association with ID. In this study, we tried to prove the ability of extended parameters of red cells and reticulocytes to diagnose ID without being influenced by FID. Design and methods: 164 chronic hemodialysis patients (CHP) in end stage renal disease were enrolled. Research parameters of red cells and reticulocytes determined on ADVIA 2120i were studied in the diagnosis of ID associated or not with chronic inflammation. Results: Parameters such as corpuscular hemoglobin of mature red cells (CHm), corpuscular hemoglobin of reticulocytes (CHr), cellular concentration of hemoglobin in mature red cells (CHCMm), cellular concentration of hemoglobin in reticulocytes (CHCMr) and percentage of microcytic and hypochromic red cells (HYMI) showed a high sensitivity to diagnose ID. However, the distinction of combined iron deficiency (CID) from other entities was not possible with all parameters. In chronic inflammatory states, the decrease of CHm, CHCMm and CHCMr with the rise of percentage hypochromic mature red cells (HYPOm) and reticulocytes (HYPOr) is in favor of CID. So, determination of inflammatory state is needed to complete research parameters of blood count in CHP. Conclusion: Extended erythrocyte and reticulocyte parameters can be useful to check iron status in CHP.
Pooling samples for serological testing was used first during the second world war. It was described later as a cost-effective technique permitting large screening of populations, especially for new infectious diseases. However, the dilution effect is responsible for decreasing sensitivity, limiting its use in practice, especially in blood banking. In this paper, we describe a modification of the classic enzyme-linked immunosorbent assay (ELISA) procedure, which permits the test of indefinite samples using just one well. Specimens are tested pure one by one without any dilution, so sensitivity remains unchanged. This new procedure is time-consuming but can be considered as a revolution in qualitative ELISA testing.
Introduction. The ADVIA 2120i is one of the most efficient analyzer used in hematology laboratories. In addition to the quantification of cells; it enables the detection of cytological abnormalities and alert biologist by specific alarms. Material and methods. We conducted a prospective study in the EHU Oran for 3 month. Overall, 146 blood samples were analyzed for which the analyser reported various cytological alarms and we reviewed them under the microscope as a gold standard. Results. This study allowed us to determine how the ADVIA 2120i can be helpful for routine use in screening cytological abnormalities. According to our results it turned out that the analyser is efficient for erythrocyte morphological abnormalities such as microcytosis, macrocytosis, hypochromy and anisocytosis. However, concerning leukocytes; we found a high rate of false positive cases, the specificity should be improved especially for detection of blasts.
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