The presence of bovine parainfl uenza virus type 3 (BPIV3) was examined in 119 nasal swabs collected from cattle with severe respiratory infection. All samples were conducted for virus isolation on the MDBK cell line. The cytopathic effect was observed after 48h to 72h in cells inoculated with eight samples (8/119; 6.7%). The confi rmation of isolated strains of BPIV3 was done by the virus-neutralization test. In addition, all samples of bovine nasal swabs were also examined for the presence of BPIV3 virus using RT-PCR with primers specifi c for the part of HN gene. The presence of BPIV3 was detected in eight samples (8/119; 6.7%) that were also positive upon virus isolation. The molecular characterization based on nucleotide sequencing of the part of the HN gene showed that all BPIV3 isolates belonged to genotype C of BPIV3. They branched in one distinct cluster with three different branches, but these branches were very similar to each other (98.1% to 99.8%). Serbian BPIV3c isolates were most similar to the Chinese BPIV3c isolates SD0805, SD0809 and SD0835 (from 97.92% to 99.7%), and to South Korean (12Q061), Japanese (HS9) and American (TVMDL16 and TVMDL20) BPIV3c strains (from 97.1% to 98.8%), and distinct from American (TVMDL15and TVMDL17) and Australian (Q5592) BPI3V genotype B strains (only 79.9% to 82.3% similarity), as well as from the genotype A BPIV3 strains from different countries published in GenBank.
Cervical cancer incidence and mortality rates in Serbia are among the highest
in Europe and data on Human papilloma virus (HPV) type distribution are
scarce. The aim of this study was to determine the prevalence of HPV types
in archival specimens of cervical cancer tissues of women in the Serbian
population. A total of 45 paraffin-embedded tissue samples of cervical
carcinoma were used in this study. The procedure included deparaffinization
of tissue samples, DNA extraction, PCR, gel electrophoresis and HPV
genotyping by direct sequencing. HPV was detected in 32 samples (71%).
Genotyping revealed the presence of 6 high-risk HPV types 16, 18, 33, 45, 53
and 58, where HPV type 16 was the most prevalent type (73.7%). The results
of this study and further studies will provide more detailed information
about HPV genotype distribution and may contribute to the formulation of
national guidelines for the prevention of cervical cancer. [175073]
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