High-risk human papilloma virus genotypes in cervical carcinoma of Serbian women: Distribution and association with pathohistological findings

Stamenković, Miodrag; Knežević, Aleksandra; Knežević, Ivana; Kuzmanovic, I; Karalić, Danijela; Milenković, Sanja; Jovanović, Tanja

doi:10.1016/j.biologicals.2016.05.001

Cited by 10 publications

(5 citation statements)

References 22 publications

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“…It confirmed the known highly oncogenic potential of type 16 since this type was significantly associated with HSIL. The previous research from Serbia reported type 16 to be the most prevalent HPV type in both cervical SCC and adenocarcinoma [25].…”

Section: Discussionmentioning

confidence: 99%

The impact of environmental and behavioural cofactors on the development of cervical disorders in HR-HPV-infected women in Serbia

Tasic¹,

Lazarević

Knežević

et al. 2018

Epidemiol. Infect.

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Persistent infection with one or more highly oncogenic human papillomaviruses (HPVs) or high-risk-HPV (HR-HPV) is necessary but not a sufficient aetiological agent for the development of cervical neoplasia. A number of viral, host, environmental and behavioural factors are suggested to be associated with the progression of cervical disorder. This study aimed to evaluate the impact of environmental and behavioural cofactors on the development of cervical disorders in HR-HPV-infected women in Serbia. A total of 541 women have been tested by PCR for the presence of HPV on the cervix. HPV genotypes were determined by direct DNA sequencing. Women identified as HR-HPV-positive were further classified into four subgroups according to their cytological status. All relevant information about demographical and behavioural factors was obtained by interviewer-based questionnaire. A number of analytical and descriptive statistical methods were used for processing the data. The cofactors found to be of significance for the progression of cervical disease were older age, body mass index >25, lower educational level, long-term smoking, previous genital infections and cervical interventions. On the other hand, condom use was found to have a protective role. Information about these cofactors might be very important for the development of more efficient cancer prevention programmes and promotion of anti-HPV vaccination.

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Section: Discussionmentioning

confidence: 99%

The impact of environmental and behavioural cofactors on the development of cervical disorders in HR-HPV-infected women in Serbia

Tasic¹,

Lazarević

Knežević

et al. 2018

Epidemiol. Infect.

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“…There are very few data on HPV testing in Serbia. In 2016, we published a study on a sample of 80 women, comparing the type of HPV positivity and morphological changes in the cervix of women operated on for cancer [42]. In our study, type 16 was present in 80.39%.…”

Section: закључакmentioning

confidence: 68%

“…Веома је мало података о HPV тестирању у Србији. Ми смо публиковали 2016. године једну студију на узорку од 80 жена, поредећи тип HPV позитивности и морфолошке промене грлића оперисаних жена од карцинома [42]. У нашој студији у 80,39% је био заступљен тип 16.…”

Section: Screening In the Republic Of Serbiaunclassified

HPV detection and/or cytological diagnostics

Milenković¹

2022

Glas javnog zdr

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The aim of this review article is to demonstrate the role and importance of the pathologist/cytologist in screening and to emphasise the importance of introducing the state-of-the-art molecular biology methods into the screening programme, in addition to providing an overview of the screening methods. Dr Tedros Adhanom Ghebreyesus, World Health Organization Director-General, announced a global call for action to eliminate cervical cancer and made it a public health priority in 2018. Cervical screening tests can detect precancerous lesions of the cervix in apparently healthy, asymptomatic women. Many institutions still use the conventional or Pap test, while many others have started using the modern test i.e. liquid-based cytology, which significantly reduces the pre-analytical phase errors. To date, studies comparing the two techniques have not provided consistent evidence that liquid-based cytology offers significant improvements in sensitivity or specificity, so both are considered acceptable. With the discovery of the etiological link between the HPV virus and cervical cancer in 1983, new methods were developed for cervical cancer screening by designing tests aimed at identifying the virus/infection rather than at the onset of the disease and detecting altered cells. The application of the HPV test as the first line of the screening programme is becoming routine. The advantages of applying the molecular technique are that it has a high predictive value, high reproducibility and high throughput. In the Republic of Serbia, the screening programme has been implemented by the National Decree since 2013, as an organised, decentralised programme and has its own difficulties in terms of personnel, financial and organisational deficiencies. The HPV primary screening would enable those difficulties to be overcome to a considerable degree, especially if liquid-based cytology were to be implemented and digital pathology introduced along with HPV screening. All of this, however, has one prerequisite, which is centralisation. Looking at the situation also from the aspect of introducing vaccines against the HPV virus, I believe that we are on the right track to control cervical cancers, and to eradicate them in the not so distant future.

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“…Todas las muestras fueron amplificadas para los primers MY09 y MY11, y un control interno de calidad para verificar si fue ADN amplificable (betaglobina humana) con los primers PC04/ GH20, para observar sus componentes. Todas las muestras se amplificaron para las enzimas consenso de restricción de 450 pb del gen L1 del VPH, MY09: (5'-CGTCCMARRGGAWACTGATC-3') y MY11: (5'-GCMCAGGGWCATAAYAATGG-3') (7)(8)(9)(10) , y se usó como control interno, hemoglobina subunidad beta (HBB) GH20: (5′-GAAGAGCCAA-GGACAGGTAC-3′) y PC04: (5′-CAACTTCATCCACG-TTCACC-3′) de 268 pb (11) .Si una muestra contenía Con relación a la electroforesis y visualización de banda, las muestras resuspendidas fueron examinadas por electroforesis en un gel de agarosa al 1,5 %, usando un marcador de peso molecular de 100 pares de bases. Posteriormente, se llevó a corrida electroforética a 70 V por 10 minutos y luego a 120 V por 45 minutos.…”

Section: Métodosunclassified

Virus papiloma humano y factores asociados en pacientes con citología desconocida atendidas en el norte de Perú

Iglesias-Osores¹,

Serquén-López²

2020

Rev peru ginecol obstet.

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Introducción. El virus papiloma humano es el causante del cáncer de cuello uterino, uno de los cánceres más comunes en las mujeres. Objetivo. Determinar la prevalencia del virus papiloma humano y los factores asociados en mujeres con citología desconocida. Métodos. En pacientes de ginecoobstetricia con citología desconocida atendidas en el Hospital Regional Lambayeque, en la costa norte del Perú, entre abril y junio 2019, se realizó la extracción de ADN para identificar el virus del papiloma humano basada en el método de salting out. Se procesaron las muestras mediante reacción en cadena de la polimerasa y se amplificaron para los primers MY09 y MY11, y PC04/GH20. Se empleó el análisis bivariado mediante la prueba de chi cuadrado y t-student. Resultados. Se encontró que 29,9% de las pacientes atendidas en el área de gineco-obstetricia con citología desconocida tuvieron el virus del papiloma humano. No se encontró diferencia estadística significativa entre la infección por virus del papiloma humano con la edad, edad de primera relación sexual, promiscuidad, número de partos vaginales, lesión de cuello uterino, antecedente de ITS, uso de anticonceptivo hormonal, uso del condón y tabaquismo.

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High-risk human papilloma virus genotypes in cervical carcinoma of Serbian women: Distribution and association with pathohistological findings

Cited by 10 publications

References 22 publications

The impact of environmental and behavioural cofactors on the development of cervical disorders in HR-HPV-infected women in Serbia

The impact of environmental and behavioural cofactors on the development of cervical disorders in HR-HPV-infected women in Serbia

HPV detection and/or cytological diagnostics

Virus papiloma humano y factores asociados en pacientes con citología desconocida atendidas en el norte de Perú

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