Distribution of Human papilloma virus genotypes in cervical cancer tissues

Stamenković, Miodrag; Knežević, Aleksandra; Kuzmanovic, I; Karalić, Danijela; Jovanović, Tanja

doi:10.2298/abs1402573s

Cited by 1 publication

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, a prevalence of 69.8% of HPV-DNA in the evaluated cases of cervical cancer tissues was observed. This finding is similar to observations made in other studies conducted in Ethiopia (67.1%) [14] and Serbia (71.1%) [15]. Higher prevalence rates of HPV were reported in studies carried out in Croatia (92-7%) [11], USA (91.3%) [16], India (93.0%) [17], Thailand (94.8%) [18], Malawi (97.0%) [19], South Africa (92.1%) [20] and Ibadan Nigeria (90.7%) [21].…”

Section: Discussionsupporting

confidence: 93%

“…A similar observation was reported in studies from two African countries-Malawi and Ghana having multiple HPV infections that accounted for 54.0% and 52.2% respectively [19, 25]. Lower rates of multiple HPV infection were reported in studies from Croatia [11], Serbia [15], India [17], Thailand [18], Ibadan Nigeria [21], Pakistan [35], Republic of Benin [41], USA [42], Brazil [43], Korea [44] and Sri Lanka [45]. Although most of the studies from Africa have observed multiple HPV infections, the finding in this study was as expected.…”

Section: Discussionsupporting

confidence: 80%

See 1 more Smart Citation

Prevalence of human papillomavirus genotypes in cervical cancer in Maiduguri, Nigeria

Kabir¹,

Bukar²,

Nggada³

et al. 2019

Pan Afr Med J

View full text Add to dashboard Cite

IntroductionCervical cancer is the commonest gynaecological malignancy and the second most common cancer among women worldwide. Several epidemiological, clinical and molecular studies have strongly implicated oncogenic high-risk human papillomavirus infection in the aetiopathogenesis of cervical cancer. The objectives of this study were to determine the cervical HPV prevalence and genotype distribution in cervical cancer in Maiduguri, Nigeria.MethodsThis was a descriptive and retrospective study. Sixty-three archived paraffin-embedded tissue blocks with confirmed diagnoses of cervical cancer during the study period (2013-2015) were retrieved and examined. The procedure included deparaffinization of tissue samples, DNA extraction, PCR, gel electrophoresis, and HPV genotyping by reverse hybridization line probe assay.ResultsSixty-three cervical cancer cases were subjected to genomic DNA extraction and HPV-DNA detection by PCR. Fifty-eight samples showed PCR positivity while 5 samples were PCR negative. HPV-specific DNA was detected in 44 of the 58 PCR-positive samples and thus the prevalence was 69.8%. Ten different high-risk HPV genotypes were detected. Both single and multiple high-risk HPV infections were observed. The most prevalent type of the human papillomavirus detected was HPV16.ConclusionHPV-DNA was prevalent in majority of the examined cervical cancer tissues and that HPV16, HPV18, HPV45, HPV51 and HPV52 were the predominant HPVs detected in both single and multiple HPV infections. The results of this study and further studies will provide more detailed information about HPV and may contribute significantly to the prevention of cervical cancer through primary high-risk HPV testing and HPV vaccination against the oncogenic viruses.

show abstract