IntroductionCervical cancer is the commonest gynaecological malignancy and the second most common cancer among women worldwide. Several epidemiological, clinical and molecular studies have strongly implicated oncogenic high-risk human papillomavirus infection in the aetiopathogenesis of cervical cancer. The objectives of this study were to determine the cervical HPV prevalence and genotype distribution in cervical cancer in Maiduguri, Nigeria.MethodsThis was a descriptive and retrospective study. Sixty-three archived paraffin-embedded tissue blocks with confirmed diagnoses of cervical cancer during the study period (2013-2015) were retrieved and examined. The procedure included deparaffinization of tissue samples, DNA extraction, PCR, gel electrophoresis, and HPV genotyping by reverse hybridization line probe assay.ResultsSixty-three cervical cancer cases were subjected to genomic DNA extraction and HPV-DNA detection by PCR. Fifty-eight samples showed PCR positivity while 5 samples were PCR negative. HPV-specific DNA was detected in 44 of the 58 PCR-positive samples and thus the prevalence was 69.8%. Ten different high-risk HPV genotypes were detected. Both single and multiple high-risk HPV infections were observed. The most prevalent type of the human papillomavirus detected was HPV16.ConclusionHPV-DNA was prevalent in majority of the examined cervical cancer tissues and that HPV16, HPV18, HPV45, HPV51 and HPV52 were the predominant HPVs detected in both single and multiple HPV infections. The results of this study and further studies will provide more detailed information about HPV and may contribute significantly to the prevention of cervical cancer through primary high-risk HPV testing and HPV vaccination against the oncogenic viruses.
Camelus dromedarius dental disorders were surveyed using abattoir samples from Maiduguri, Nigeria. A total of 313 heads, were randomly examined. The age and sex of the heads were estimated using standard methods and disorders were observed following the split of the oral commissure. Heads with multiple disorders were counted once and of 313 heads, 114 (41.67 %) and 199 (58.33 %) were males and females, respectively. Heads aged <5 years was 18.21 %; 5-10 years, 44.41 %; and >15 years was 37.38 %. Whereas 22.61 % of the total heads had one disorder or another, 7.98 and 14.06 % of the disorders belonged to male and female heads, respectively. The prevalence rate of the disorders observed include dental tartar and calculus (5.42 %), inward rotation of incisors (2.87 %), fractured teeth (7.66 %), maleruption (0.31 %), oligodontia (0.31 %), gingivitis (4.15 %), ulcerated cheek(0.63 %), and presence of foreign body(0.31 %) in the mouth. Prevalence rate of oral-cavity abnormalities in relation to age of the total heads is 5.75, 5.11, and 10.54 % for animals <5, between 5 and 10 years, and 10-15 years, respectively. Furthermore, heads that were affected with various types of disorders are 31.58 % (<5 years of age), 11.51 % (aged 5-10 years), and 28.21 % (>15 years). The study serves as a call for increased oral-cavity health care of camels.
Borno state is the second largest state in Nigeria with over 70,000 square kilometers of diverse ecosystems including parts of the fertile Lake Chad basin. However, more than 2/3 of this landmass is threatened with drought, advancing desertification and degraded soils. Most restoration efforts involve revegetation, which in the past has met with limited success. Microbial communities of soils play a pivotal role in soil fertility and plant cover. We conducted the first metagenomic amplicon sequencing study, comparing two soil depths to determine whether soil bacteria abundance and diversity in the harsh bare soils were sufficient to sustain greening efforts. The goal was to glean insights to guide microbial inoculant formulation needed in the region. Samples from top (0-15 cm) and sub (16-65 cm) soils were collected from five strategic locations in the state. Using next generation Illumina sequencing protocols, total DNA extracted directly from the soils was sequenced and analyzed by QIIME. Metadata collected from site showed scorching temperatures of over 46˚C, near zero moisture level and a pH of about 6 for top soil. At 65 cm depth, the temperature averaged 32˚C with a pH of 5 and significantly higher soil moisture of 0.1%. The bacterial community structure was unexpectedly very diverse at both soil depths samples, recording a ChaO1 index ranging from 909 to 4296 and a Shannon diversity range of 3.54 to 6.33. The most abundant phyla in both soil depths were the Firmicutes and Proteobacteria; however the relative abundance of composite lower taxa was strikingly different. Operational taxonomic units and diversity indices were highest How to cite this paper:
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