This study aimed to evaluate the inflammatory response of the subcutaneous tissue of twenty rats when the use of iodoform with different drugs or not associated with laser photobiomodulation. In the dorsum of rats were introduced into tubes containing: Iodoform and calcium hydroxide (Ca (OH)2); Iodoform and Ca(OH)2, associated with Laser; Iodoform and Otosporin; Iodoform and Otosporin, associated with Laser. The animals were euthanized eight and fifteen days after surgery, the parts were removed and processed in the laboratory with staining with Hematoxylin-Eosin (HE) and Picrosirius Red. The statistical significances of the inflammatory infiltrate and quantification of collagen fibers were measured by ANOVA-Tukey test. The association between Ca (OH)2 and iodoform with determined the best response in the host tissues. The low level laser therapy was effective in modulating the inflammatory response and deposit collagen fibers
ABSTRACT:This study aimed to evaluate the cytotoxicity of intracanal medications on L929 fibroblast cells at different periods of observation. The following experimental groups were studied: calcium hydroxide with camphorated paramonochlorophenol and glycerin (CPG); iodoform with glycerin (IG); calcium hydroxide with iodoform and distilled water (CIW); iodoform with distilled water (IW); calcium hydroxide with distilled water (CW); Otosporin ® (OT); and a control group composed of cells and culture medium. Eluates were prepared from each group and placed in contact with 1 x 10 5 cells/well for periods of 30 minutes, 12, 24, 48 and 72 hours, 5 and 7 days. After each experimental period, a cytotoxicity test was performed using methyltetrazolium (MTT) and a spectrophotometer at an optical density of 570 nm to analyze cell viability. The ANOVA and Tukey test with a significance level of 5% was used to analyze the data. At 30 minutes and at 12 hours, all groups were equal to the control group. At 24 hours, there was greater cytotoxicity in the IG group than in the control group (P<0.001). At 48 hours, only the OT group was cytotoxic (P <0.001). At 72 hours and at 5 days, the most cytotoxic groups were CW and OT. At 7 days, the IW and CPG groups were the least cytotoxic (P <0.001). With respect to experimental time, significant differences between 24 hours and 7 days were observed in all groups. Otosporin ® was the most cytotoxic medication, followed by calcium hydroxide with distilled water.
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