Recently, the modification of the initial structure of biopolymers, mainly chitosan, has been gaining importance with a view to obtain functional forms with increased practicality and specific properties enabling their use in tissue engineering. Therefore, in this article, the properties (structural and biological) of thermosensitive hydrogels obtained from chitosan lactate/chloride and two types of crosslinking agents (β-glycerol phosphate disodium salt pentahydrate and uridine 5′-monophosphate disodium salt) are discussed. The aim of the research is to identify changes in the structure of the biomaterials during conditioning in water. Structural investigations were carried out by FTIR spectroscopy. The crystallinity of gels was determined by X-ray diffraction analysis. The biocompatibility (evaluation of cytotoxicity and genotoxicity) of chitosan hydrogels was investigated by contact with human colon adenocarcinoma cell line for 48 h. The cytotoxicity was verified based on the colorimetric resazurin assay, and the genotoxicity was checked by the comet assay (percentage of DNA in the comet tail). The conducted research showed that the analyzed types of chitosan hydrogels are non-cytotoxic and non-genotoxic materials. The good biocompatibility of chitosan hydrogels surfaces makes them interesting scaffolds with clinical potential in tissue regeneration engineering.
The paper presents research results on the crystallization kinetics of polycaprolactone (PCL) with micro-and nano-additives such as talc (nucleation agent) and nanosilver (antibacterial agent). The process of isothermal crystallization was studied in a narrow temperature range 38.5-41.5°C using Differential Scanning Calorimetry (DSC) and Thermooptical Analysis (TOA) methods. The kinetics of the process are described using the Avrami model. The parameters n and log K of the equation were calculated. Changes in the half time of crystallization, degree of crystallinity and melting temperature of the polymer under the influence of the fillers and the conditions of crystallization were analyzed.
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The currently observed development of medical science results from the constant search for innovative solutions to improve the health and quality of life of patients. Particular attention is focused on the design of a new generation
of materials with a high degree of biocompatibility and tolerance towards the immune system. In addition, apart from
biotolerance, it is important to ensure appropriate mechanical and technological properties of materials intended for intrabody application. Knowledge of the above parameters becomes the basis for considerations related to the possibilities of
choosing the appropriate polymer materials. The researchers' interest, as evidenced by the number of available publications, is attracted by nanobiocomposites based on chitosan and carbon nanotubes, which, due to their properties, enable
integration with the tissues of the human body. Nanosystems can be used in many areas of medicine. They constitute an
excellent base for use as dressing materials, exhibiting antimicrobial properties. In addition, they can be carriers of drugs
and biological macromolecules, and can be used in gene therapy, tissue engineering and construction of biosensors. For
this reason, potential application areas of chitosan-carbon nanotube nanocomposites in medical sciences are presented in
this publication, considering the characteristics of the system components.
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