Influence of chitosan average molecular weight on degradation and stability of electrodeposited conduits

Nawrotek, Katarzyna; Tylman, Michał; Adamus-Włodarczyk, Agnieszka; Rudnicka, Karolina; Gatkowska, Justyna; Wieczorek, Marek; Wach, Radosław A.

doi:10.1016/j.carbpol.2020.116484

Cited by 20 publications

(20 citation statements)

References 39 publications

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“…Then, THP1-Blue™ NF-κB monocytes were re-cultured in selective RPMI 1640 medium additionally containing 10 µg/mL blasticidin in a density below 1 × 10 6 cells/mL at 37 °C, and in a humidified atmosphere containing 5% CO 2 . The experiments were conducted as described previously [ 32 , 33 ]. Briefly, monocytes were adjusted to the optimal density (1 × 10 6 cells/mL) and distributed equally in each well of the 96-well plate (200 µL/well).…”

Section: Methodsmentioning

confidence: 99%

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Composites Based on Hydroxyapatite and Whey Protein Isolate for Applications in Bone Regeneration

et al. 2021

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Hydroxyapatite (HAp) is a bioactive ceramic with great potential for the regeneration of the skeletal system. However, its mechanical properties, especially its brittleness, limit its application. Therefore, in order to increase its ability to transmit stresses, it can be combined with a polymer phase, which increases its strength without eliminating the important aspect of bioactivity. The presented work focuses on obtaining organic–inorganic hydrogel materials based on whey protein isolate (WPI) reinforced with nano-HAp powder. The proportion of the ceramic phase was in the range of 0–15%. Firstly, a physicochemical analysis of the materials was performed using XRD, FT-IR and SEM. The hydrogel composites were subjected to swelling capacity measurements, potentiometric and conductivity analysis, and in vitro tests in four liquids: distilled water, Ringer’s fluid, artificial saliva, and simulated body fluid (SBF). The incubation results demonstrated the successful formation of new layers of apatite as a result of the interaction with the fluids. Additionally, the influence of the materials on the metabolic activity according to ISO 10993-5:2009 was evaluated by identifying direct contact cytotoxicity towards L-929 mouse fibroblasts, which served as a reference. Moreover, the stimulation of monocytes by hydrogels via the induction of nuclear factor (NF)-κB was investigated. The WPI/HAp composite hydrogels presented in this study therefore show great potential for use as novel bone substitutes.

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Section: Methodsmentioning

confidence: 99%

“…conducted as described previously [32,33]. Briefly, monocytes were adjusted to the optimal density (1 × 10 6 cells/mL) and distributed equally in each well of the 96-well plate (200 µL/well).…”

Section: X-ray Diffraction Analysis Of Hapmentioning

confidence: 99%

Composites Based on Hydroxyapatite and Whey Protein Isolate for Applications in Bone Regeneration

et al. 2021

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“…Polysaccharide's degradation into the mammalian body is generally driven by two different routes: hydrolytic and enzymatic degradation 69 . In both cases there is a cleavage of the glycosidic bond between the polysaccharide units.…”

Section: Enzymatic Biodegradationmentioning

confidence: 99%

Microwave-assisted methacrylation of chitosan for 3D printable hydrogels in tissue engineering

et al. 2022

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“…It is also an environmentally friendly method. 64,65 The enzyme specific chitosanase can be used in the hydrolysis of β-1,4-glycosidic bonds, as well as non-specific chitinases 77 , lysozym 78 or cellulases. 71 The reduction of the CS molecular weight under the influence of the protease enzyme changed the structure of the oligomer, but did not affect the deacetylation degree.…”

Section: Chitosan Degradationmentioning

confidence: 99%

Biomimetic scaffolds based on chitosan in bone regeneration. A review.

Kołakowska

Gadomska‐Gajadhur

Ruśkowski³

2021

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The article focuses on a polysaccharide of natural origin – chitosan and its application in tissue engineering. The preparation process and physicochemical properties of the saccharide are described. The degradation of chitosan and the properties influencing the process both outside and in living organism were examined. Four applications in bone tissue engineering can be distinguished: preparation of cell scaffolds exclusively from chitosan, from a chitosan composite or from a chitosan polyelectrolyte complex. The fourth way is to modify the surface of scaffolds made of other materials by covering them with a layer of chitosan. At the end of the article, the processes taking place after placing the implant inside the body are described, how the structure of chitosan affects the behaviour of bone cells in the adhesion process and life processes.

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Influence of chitosan average molecular weight on degradation and stability of electrodeposited conduits

Cited by 20 publications

References 39 publications

Composites Based on Hydroxyapatite and Whey Protein Isolate for Applications in Bone Regeneration

Composites Based on Hydroxyapatite and Whey Protein Isolate for Applications in Bone Regeneration

Microwave-assisted methacrylation of chitosan for 3D printable hydrogels in tissue engineering

Biomimetic scaffolds based on chitosan in bone regeneration. A review.

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