Physico-Chemical Properties and Biocompatibility of Thermosensitive Chitosan Lactate and Chitosan Chloride Hydrogels Developed for Tissue Engineering Application

Abstract

Recently, the modification of the initial structure of biopolymers, mainly chitosan, has been gaining importance with a view to obtain functional forms with increased practicality and specific properties enabling their use in tissue engineering. Therefore, in this article, the properties (structural and biological) of thermosensitive hydrogels obtained from chitosan lactate/chloride and two types of crosslinking agents (β-glycerol phosphate disodium salt pentahydrate and uridine 5′-monophosphate disodium salt) are discussed. The aim of the research is to identify changes in the structure of the biomaterials during conditioning in water. Structural investigations were carried out by FTIR spectroscopy. The crystallinity of gels was determined by X-ray diffraction analysis. The biocompatibility (evaluation of cytotoxicity and genotoxicity) of chitosan hydrogels was investigated by contact with human colon adenocarcinoma cell line for 48 h. The cytotoxicity was verified based on the colorimetric resazurin assay, and the genotoxicity was checked by the comet assay (percentage of DNA in the comet tail). The conducted research showed that the analyzed types of chitosan hydrogels are non-cytotoxic and non-genotoxic materials. The good biocompatibility of chitosan hydrogels surfaces makes them interesting scaffolds with clinical potential in tissue regeneration engineering.

Screening of Self-Assembling of Collagen IV Fragments into Stable Structures Potentially Useful in Regenerative Medicine

“…All of the analyzed samples and controls were then incubated with collagen IV fragments for 48 h. Following incubation of cells, the well contents were removed and wells were rinsed with 1 X DPBS. Next, 100 µL of the resazurin solution (10% of cell culture medium volume) was added to each well and incubated with the resazurin solution for 2 h. Absorbance was measured at a wavelength of 600 nm and at a reference wavelength of 690 nm using a Synergy HT (BioTek) spectrophotometer [93]. Cell viability was calculated using the following formula:…”

Chitosan-Based Thermo-Responsive Scaffold for Wound Heal-ing Provides Dexketoprofen Trometamol Controlled Release for 24 Hour-Use

“…In the near IR region, DK strong bands are seen in the three formulations around 1060 cm -1 with vibrations at 1568, 1538 and 771 cm -1 , which are also indicative of the raw material purity [24]. Chitosan's O-H stretching close to 3300 cm -1 and the C-H vibrations close to 2900 cm -1 are observed in the three formulations [25,26]. Regarding F2, C-H alkyl stretching of PVA can be observed near 2900 cm -1 , and also, its typical broad band at 3000-3500 cm -1 [27].…”

Chitosan as an Underrated Polymer in Modern Tissue Engineering

“…Silica has many valuable properties for polymers. One of them is the tolerance to the growth of microorganisms as well as the large specific surface area [185]. Chitosan-silica-based composites may be a potential carrier for mono-, di-, and triphosphate nucleotides [186].…”