Acne vulgaris adalah suatu kondisi inflamasi umum pada unit polisebaseus, ditandai dengan komedo, papul, pustul atau nodul. Penyakit kulit ini bukan merupakan penyakit yang berbahaya tetapi mempunyai dampak yang besar secara fisik maupun psikologik. Prinsip penanganan acne antara lain  menurunkan populasi Propionibacterium acne dan menekan inflamasi. Dari penelitian sebelumnya ditemukan adanya mikroorganisme lainnya dalam lesi yang mungkin berperan selain Propionibacterium acne yaitu Staphylococcus aureus, S. epidermidis dan Pityrosporum ovale. Beberapa sumber melaporkan ada beberapa manfaat Aloe vera untuk kecantikan dan perawatan kulit. Aloe vera juga digunakan secara eksternal untuk mengobati berbagai kondisi kulit seperti luka, nyeri dan menekan proses inflamasi. Penelitian ini dilakukan untuk mengetahui efek antimikroba ekstrak Aloe vera konsentrasi 25%,50% dan 75% terhadap isolat bakteri penyebab Acne vulgaris secara invitro dengan menggunakan metode difusi. Sebelum pengujian dilakukan isolasi dan identifikasi bakteri dari lesi. Bakteri yang ditemukan dari lesi penderita tergolong bakteri golongan Gram positif yaitu  Staphylococcus aureus dan Propionibacterium acne. Hasil uji One-way Anova  menunjukan terdapat perbedaan bermakna efektivitas ekstrak lidah buaya terhadap S.aureus dan P.acne. Uji Post Hoc terhadap S.aureus  menunjukan terdapat perbedaan bermakna ELB 25%, 50% dan 75% dengan kontrol (+) dan kontrol (-), tidak terdapat perbedaan bermakna antara ELB 25% dengan 50% dan 50% dengan 75%. Sedangkan antara 25% - 75% berbeda bermakna. Sedangkan Uji Post Hoc terhadap P.acne menunjukan bahwa tidak terdapat perbedaan bermakna antara  ELB 25%, 50% dan 75% dengan kontrol positif. Kesimpulan : Ekstrak Lidah Buaya (Aloe vera)  mempunyai efek antimikroba terhadap isolat bakteri penyebab Acne vulgaris yaitu Propionibacterium acne dan Staphylococcus aureus  pada konsentrasi 25%, 50% dan 75% secara invitro.

Shigella dysenteriae is pathogenic bacteria causes dysentery diarrhea, a diarrhea with mucus and blood in the feces. Currently, drugs or foods containing Lactobacillus have been circulated in the markets and used by people. This research aims to know the in vitro inhibition test of L. plantarum metabolite substances filtrate against growth of S. dysenteriae. This research used experimental design with L. plantarum metabolite substances filtrate as the samples. Antibacterial test method which was used was the cup-plate technique by looking at clear zone of bacteria's growth around cup-plate and used Mueller Hinton Agar media. The results showed clear zones on filtrate with concentrations 25%, 50%, 75%, and 100% with average inhibition zones 0,284 cm; 0,332 cm; 0,406 cm; and 0,619 cm. Kruskal-Wallis test results indicated there were significant differences of each filtrate concentration against S. dysenteriae (p<0,05). Post Hoc test showed no significant difference between concentrations 25% and 50% (p>0,05). This suggested lactic acid, acetic acid, and bacteriosin presented in L. plantarum metabolite have antibacterial characteristic. Keywords: antibacterial, Lactobacillus plantarum, Shigella dysenteriae INTISARIShigella dysenteriae merupakan bakteri patogen penyebab diare disentri, yaitu kondisi diare disertai lendir dan darah dalam feses. Saat ini, sediaan obat atau makanan yang mengandung Lactobacillus telah beredar di pasaran dan dimanfaatkan oleh masyarakat. Tujuan penelitian ini adalah untuk mengetahui daya hambat filtrat zat metabolit L. plantarum terhadap pertumbuhan S. dysenteriae secara in vitro. Penelitian ini menggunakan desain eksperimental dengan sampel filtrat zat metabolit L. plantarum. Metode uji antibakteri yang digunakan adalah metode sumuran dengan melihat zona hambat pertumbuhan bakteri di sekitar sumuran. Hasil penelitian ini memperlihatkan adanya zona bening pada konsentrasi filtrat 25%, 50%, 75%, dan 100% dari pertumbuhan S. dysenteriae dengan rata-rata zona hambat 2,84 mm; 3,32 mm; 4,06 mm; dan 6,19 mm. Hasil uji Kruskal-Wallis menunjukkan perbedaan signifikan masing-masing konsentrasi filtrat terhadap S. dysenteriae (p<0,05). Pada uji Post Hoc, kelompok konsentrasi 25% dan 50% menunjukkan tidak terdapat perbedaan signifikan (p>0,05). Penelitian ini menunjukkan bahwa asam laktat, asam asetat, dan bakteriosin yang terdapat pada metabolit L. plantarum dapat bersifat sebagai antibakteri.Kata kunci: antibakteri, Lactobacillus plantarum, Shigella dysenteriae PENDAHULUANDiare merupakan masalah kesehatan dunia karena morbiditas (angka kesakitan) dan mortalitasnya (angka kematian) yang tinggi serta merupakan salah satu dari tiga penyakit utama selain pneumonia dan malaria, yang menyebabkan kematian pada anak-anak (WHO, 2012). Diare adalah suatu kondisi buang air besar dengan konsistensi lembek atau cair, bahkan dapat berupa air

Background: Covid-19 is a highly infectious disease. Primary prevention approaches, including personal protective measures, social distancing, and environmental surface cleaning, are required to contain disease transmission. This study aimed to investigate the compliance in implementing Covid-19 health protocols among cadres at Community Health Centre, Depok, West Java. Subjects and Method: This was a descriptive cross-sectional study conducted at Community Health Centre, Depok, West Java. A total of 73 cadres was selected for this study. The study variables were compliance on Covid-19 protocols, including mask-wearing, hand hygiene, physical and social distancing, level of education, level of Covid-19 knowledge, and other personal risk factors. The study subjects were selected by total sampling. The primary data were collected via WhatsApp group using questionnaires. The data were reported descriptively. Results: Two-third of study subjects were at age 40-50 years (61.64%). The majority of subjects were junior high school educational level (74.4%). All the study subjects complied to wear a face mask (100%). The majority of cadres possessed a high level of Covid-19 knowledge (57.53%). Most of the cadres were unemployment (72.88%). The common personal health problem was digestive problems (10.96%). Conclusion: The characteristics of cadres vary with age, educational level, level of compliance in Covid-19 protocols, work status, and level of Covid-19 knowledge. The common personal health problem among cadres is digestive problems. Keywords: compliance, Covid-19, health protocols, characteristics Correspondence: Sri Wahyuningsih. Faculty of Medicine, UPN Veteran Jakarta. Jl. Pondok Labu, Jakarta Selatan. Email: dr_sriwahyuningsih@yahoo.com. Mobile: +6281287552465. DOI: https://doi.org/10.26911/the7thicph.02.32

Latar belakang: Prevalensi dismenore diperkirakan berkisar antara 45–95 persen dan 10–25 persen diantaranya merupakan dismenore primer berat. Dismenore dapat menimbulkan sejumlah masalah, antara lain limitasi aktivitas, penurunan prestasi akademik, dan kesulitan tidur. Saat ini, terdapat kecenderungan penggunaan herbal dan pengobatan alternatif untuk mengatasi dismenore primer. Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh madu terhadap tingkat nyeri dismenore dan kualitas hidup mahasiswi Fakultas Kedokteran Universitas Pembangunan Nasional Veteran Jakarta (FKUPNVJ). Metode: Penelitian menggunakan one group pretest-posttest design. Sebanyak 30 subjek yang ditentukan dengan consecutive sampling  diminta minum madu sebanyak dua sendok makan yang dimulai dari dua hari sebelum menstruasi hingga hari ketiga menstruasi. Data diambil menggunakan kuesioner, Numeric Rating Scale, Verbal Multidimensional Scoring System, dan Brief Pain Inventory. Hasil: Hasil uji Wilcoxon menunjukkan madu dapat menurunkan intensitas nyeri (p = 0,000) dan grade dismenore (p = 0,001). Selain mengurangi derajat nyeri, madu dapat mengurangi lama waktu nyeri menstruasi dari 2 hari menjadi 1 hari (p = 0,001). Hasil uji Wilcoxon juga menunjukkan madu dapat mengurangi gangguan dismenore terhadap aktivitas secara umum, suasana hati, kemampuan berjalan, pekerjaan, hubungan dengan orang lain, tidur, dan menikmati hidup (p ≤ 0,001). Kesimpulan: madu dapat menurunkan tingkat nyeri dan meningkatkan kualitas hidup mahasiswi FKUPNVJ dengan dismenore primer.  Kata kunci: dismenore primer, kualitas hidup, madu, mahasiswi

Tobacco plants are one of the main trade commodities in Indonesia. At present, the main production of tobacco is cigarettes. However, tobacco has active antibacterial compounds, such as phenols, alkaloids, and essential oils. Therefore, tobacco can be used in the health sector. This study was conducted to determine the effectiveness of the pyrolysis extract of Nicotiana tabacum L. var Virginia in inhibiting Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa. This study uses a true experimental research design with tobacco extract samples obtained by pyrolysis at concentrations of 20%, 40%, 60%, 80%, and 100%. The antibacterial test carried out was the Kirby-Bauer diffusion method on Mueller Hinton Agar (MHA) media. One-Way ANOVA test results with p < 0.05 indicate the effectiveness of tobacco pyrolysis extract in inhibiting Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa. The average yield of inhibition zones found in Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa were 6.35 mm, 5.9 mm, 3.97 mm, and 5.025 mm. From these results, Staphylococcus aureus bacteria became the most sensitive bacteria with Virginia tobacco pyrolysis extract.

Covid-19 outbreak that originated in Wuhan's city is spreading very quickly and is currently a pandemic worldwide. Transmission through droplets that come out of the patient's nose or mouth, whether symptomatic or asymptomatic.  Efforts to prevent and fight COVID-19 must be carried out jointly with the community's cooperation and require strong discipline by washing hands frequently, maintaining distance, wearing masks, staying at home, and increasing body power. The purpose of this study was to determine the level of cadre compliance  in carrying out health protocols in the era of the Covid-19 pandemic. This research method with a cross-sectional approach. The respondents were cadres of Limo Health Center, Depok. It was total sampling. The research instrument used a questionnaire of Google Form and analyzed using Chi-Square. The results showed that most of the respondents were included in the 46-60 year interval (51.4%) were highly educated, tertiary education (70%), knowledgeable (54.3%), unemployed (87.1), obeyed the protocol. Health (50%). Compliance increases in the elderly, higher education, sufficient knowledge, work status. conclusion there is no relationship between age (p = 0.632), education level (p = 0.794), knowledge level (p = 0.253), work status (p = 0.074) and compliance with the Covid-19 health protocol.

Background: Moringa oleifera fruit extract contains beneficial chemical compounds. This study was conducted to observed the power of antioxidant against liver injury by 2-Nitropropane induction in an obese male mice model. Methods: This research was in vivo laboratory experimental study with a post-test control design group only. The population was obese male mice models, Swiss strain, aged 6–8 weeks, weighing between 60–80 gr. The research sample was determined by Federer's formula for a complete randomized design experimental test, group N (control), O1 (induced by 2-Nitropropane intraperitoneal (i.p) once), O2 (induced by 2-Nitropropane i.p twice), P1 (induced by 2-Nitropropane i.p. once and gavage with M. oleifera fruit extract 500mg/kg bodyweight (BW) once a day), P2 (induced by 2-Nitropropane i.p. twice and gavage of M. oleifera fruit extract 500mg/kg BW once a day), and P3 (induced by 2-Nitropropane i.p. twice and gavage of vitamin C 500mg/kg BW once a day). Antioxidant potential parameters were measured by levels of malondialdehide (MDA), glutation (GSH), 8-hydroxy-2'-deoxyguanosine (8-OHdG), catalase activity, manganese superoxide dismutase (MnSOD), serum glutamic pyruvic transaminase (SGPT), serum glutamic oxaloacetic transaminase (SGOT). This research was held at the Biochemistry laboratory of Medicine Faculty, UPN Veteran Jakarta in May–September 2020. Analysis was carried out using SPSS version 20.0. The parameters were tested using ANOVA. Results: MDA levels decreased, GSH increased, 8-OHdG decreased, catalase activity increased, MnSOD activity increased and SGOT, SGPT levels decreased. M. oleifera fruit extract was statistically proven to be a candidate for potential antioxidant against liver injury of 2-Nitropropane induction in obese male mice model.  Conclusions: M. oleifera fruit extract was statistically evident as an antioxidant substance that reduces oxidative stress in acute liver injury caused by 2-Nitropropane induction.

Compliance with treatment is an important factor in the health of hypertensive patients. Compliance is a prerequisite for the effectiveness of hypertension therapy, whereas patient non-compliance with antihypertensive drugs is one of the main factors in the failure of therapy. There are several factors that influence medication adherence and some basic principles of therapy that need to be considered to reach the target of therapy and reduce complications of hypertension. Hypertension sufferers in the Cisalak Pasar Community Health Center are quite numerous, they are generally elementary school and not schooled (74.2%). Kidney complications and a combination of heart and kidney are also quite large, which is 40%. This study aims to determine the pattern of antihypertensive administration and the relationship of age, education level, degree of hypertension, complications and type of therapy with the level of adherence to taking medication for hypertensive patients at the Cisalak Pasar Community Health Center. The research design used in this study was a descriptive cross-sectional. The subjects of the study were essential hypertension patients at the Cisalak Pasar Health Center, Depok who met the inclusion and exclusion criteria. The results of this study are that the pattern of antihypertensive administration is in accordance with JNC 8, there is no relationship between age, education level, hypertension degree and the number of drugs taken with medication compliance, and there is a relationship between the presence or absence of complications with medication compliance