IntroductionThe purpose of the current study was to evaluate the blood glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) levels under seasonal variations in dairy cows during transition period, and to assess the relationship between chosen reproductive parameters, GSH-Px, and MDA.Material and MethodsHolstein cows calving in January were assigned into winter group (n = 42), while cows calving in August were assigned into summer group (n = 42). Blood samples were collected from the jugular vein 21, 14, and 7 days before calving, at calving (0 day), and 7, 14, and 21 days after calving. Reproductive parameters obtained from farm records were evaluated.ResultsIn both groups of cows, GSH-Px activity decreased from 21 days before calving to day 0, and it gradually continued to increase until 21 days after calving. GSH-Px activity was higher in winter group compared to summer group during the transition period (P < 0.05). MDA levels in both groups increased over time starting from 21 days before calving to 0 day, but it gradually decreased thereafter. MDA levels were higher in summer group compared to winter group during the transition periods (P < 0.05). Summer group of cows showed higher intervals of calving-to-oestrus, calving-to-conception, and higher insemination index (P < 0.01). Negative correlation was recorded between GSH-Px and MDA during all examination days (P < 0.01). MDA levels correlated with calving to conception interval on day 21 before calving and day 0 (P < 0.01) and insemination index on day 0 and 21 days after calving (P < 0.01). GSH-Px activity was negatively correlated with calving to conception interval on day 21 before calving, day 0, and 21 days (P < 0.01) after calving. Negative correlation on day 21 before calving and day 0 was also determined between GSH-Px and insemination index (P < 0.01).ConclusionThis study showed that blood oxidant and antioxidant levels have affected the fertility parameters in cows under seasonal variations.

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Introduction The objectives of this study were to determine the role of a fall in pre-calving body condition score (BCS) in postpartum metabolic status and reproductive outcomes, and gauge the indicativeness of blood metabolites during the transition period. Material and Methods Cows were grouped based on BCS loss between days −14 ±3 and 0 relative to calving. Cows that lost no BCS were the BCS control group (BCS-C), cows that lost 0.25 BCS points the low BCS loss group (BCS-L), and those that lost 0.5 points or more the high BCS loss (BCS-H) group. Blood was taken on days −14 ±3, 3, 14, and 30 ±4 for determination of comprehensive metabolic panel biomarker levels. Beta-hydroxybutyric acid (BHBA) levels were quantified on postpartum examination days. Vaginal discharge scores, ovarian activity on day 30 ±4, and subsequent fertility parameters were evaluated. Results The BCS-H cows had lower mean Ca concentrations before calving and on day 3, when the group’s BHBA and CK were higher (P < 0.05); on day 14 they had higher AST concentrations (P < 0.05). The BCS-L cows had greater bilirubin levels (P < 0.05). The BCS-H cows had lower cyclicity and higher endometritis rates. First service pregnancy rates were 50%, 50%, and 61.9%, open days 96.8, 95.75, and 89.2, and overall pregnancy rates 56.25%, 65%, and 80.95 % in the BCS-H, BCS-L, and BCS-C groups, respectively. Conclusion Prepartum BCS loss of ≥ 0.5 points could be associated with Brown Swiss cow low Ca and BHBA concentrations early postpartum, and with subsequent uterine health and overall pregnancy rate. Prepartum Ca concentration might be a prognostic biomarker for postpartum metabolic status and reproductive outcomes.

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The aim of this study was to investigate the prognosis, the risk status and the death reasons in dead and recovered bitches with pyometra after the operative approach by comparing serum biochemical, and haematological parameters, as well as bacteriological and clinical findings. Bitches with pyometra (n=30) were ovariohysterectomized. Eight bitches that died after the ovariohysterectomy formed Group 1 and the rest, which recovered, was Group 2. In addition, 10 healthy bitches were used as the reference for haematological and biochemical parameters. It was found that the level of band neutrophils in Group 1 was higher than 10% and there was lymphopenia along with a marked monocytosis resulting in a highly negative correlation (r=-0.74). There were significant differences (p<0.01) between Group 1 and 2 for BUN, creatinine and BUN/creatinine ratio. Also, the increased serum BUN and creatinine levels were significantly correlated (rs = 0.605 and rs = 0.514, respectively). A significant tendency was apparent for increasing mortality in bitches with pyometra whose BUN levels were over 30 mg/dL (odds ratio=23.80) and creatinine levels above 1.5 mg/dL (odds ratio=7.518). Clinical signs and bacteriological findings did not lead to marked differences between the groups. In pyometra cases, the elderly azotemic dogs having higher BUN and creatinine concentration, would more likely succumb within 3 days after surgery. It may be concluded that the increased BUN concentrations may serve as a reliable prognostic predictor