The comparative evaluation of serum biochemical, haematological, bacteriological and clinical findings of dead and recovered bitches with pyometra in the postoperative process

Küplülü, Şükrü; Vural, Mehmet Rıfat; Demırel, Arif Hakan; Polat, Muhammed Mirhan; Akçay, Ali

doi:10.2298/avb0903193k

Cited by 14 publications

(6 citation statements)

References 15 publications

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“…Most bacteria isolated from uterine content are gram negative bacteria that release lipopolysaccharidestructured endotoxin from their cell walls [1,3,5] . Multiple organ failure due to sepsis [6] and death may be observed in this disease [7][8][9] . Previous studies have shown that the mortality rate caused by pyometra is about 4-26% [7,9,10] .…”

Section: Introductionmentioning

confidence: 99%

“…Multiple organ failure due to sepsis [6] and death may be observed in this disease [7][8][9] . Previous studies have shown that the mortality rate caused by pyometra is about 4-26% [7,9,10] . Renal dysfunction and hepatocellular damage caused by septicemia and/or diminished hepatic circulation and cellular hypoxia in a dehydrated bitch can lead to alteration in variety of serum biochemical parameters.…”

Section: Introductionmentioning

confidence: 99%

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Köpeklerde Endotoksik Pyometranın Klinik, Bakteriyolojik ve Histopatolojik Özellikleri

Demırel¹,

Vural²,

Vural³

et al. 2018

Kafkas Univ Vet Fak Derg

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The object of this study was to compare the histopathologic change in uterus as well as the findings of clinical and bacteriological of twentyfour bitches with pyometra. Uterine lesions were grouped as acute endometritis, subacute endometritis, acute metritis, subacute metritis. Complete blood-count, blood biochemical parameters and toxin levels were determined. Only alkaline phosphatase levels were significantly different between all groups (P<0.01). Blood endotoxin level was different between the acute and subacute endometritis groups (P<0.01), and subacute endometritis and acute metritis groups (P<0.01). All bitches in the subacute endometritis group recovered. Three bitches in the acute endometritis group, two bitches in the acute metritis group and one bitch in the subacute metritis group died. The blood endotoxin level was determined to be quite high in the dead bitches. There was a difference in the levels of blood urea nitrogen (P<0.05), creatinine (P<0.01), and toxin (P<0.0001) between the dead and surviving bitches. In conclusion, the prognosis of pyometra may be recovered in bitches diagnosed with subacute endometritis associated with low levels of circulating endotoxin. The endotoxemic bitches that have blood endotoxin level above 0.96 EU/mL along with significantly increased alkaline phosphatase, blood urea nitrogen, and creatinine concentration would likely end up with a mortality outcome for acute endometritis, acute metritis, or subacute metritis. These parameters may serve as good prognostic markers in bitches with pyometra. Keywords: Bacteriology, Bitch, Histopathology, Prognosis, Pyometra Köpeklerde Endotoksik Pyometranın Klinik, Bakteriyolojik ve Histopatolojik Özellikleri ÖzBu çalışmanın amacı, pyometralı yirmi dört köpeğin uteruslarındaki histopatolojik değişikliklerin karşılaştırılmasıdır. Uterus lezyonları akut endometritis, subakut endometritis, akut metritis, subakut metritis olarak gruplandırıldı. Tam kan sayımı, kan biyokimyasal parametreler ve toksin seviyeleri belirlendi. Tüm gruplar arasındaki farklılık sadece alkalen fosfotaz seviyesinde önemliydi (P<0.01). Kan endotoksin seviyesi, akut ve subakut endometritis grupları (P<0.01) ile subakut endometritis ve akut metritis grupları (P<0.01) arasında farklıydı. Subakut endometritis grubundaki tüm köpekler iyileşti. Akut endometritis grubunda üç köpek, akut metritis grubunda iki köpek ve subakut metritis grubunda ise bir köpek öldü. Kan endotoksin seviyesinin ölen köpeklerde oldukça yüksek seviyede olduğu belirlendi. Ölen ve yaşayan köpekler arasında kan üre nitrojen (P<0.05), kreatinin (P<0.01) ve toksin (P<0.0001) seviyelerinin farklı olduğu görüldü. Sonuç olarak, dolaşımdaki endotoksinin düşük seviyesi ile ilişkili olarak subakut endometritis tanısı konulan köpeklerde pyometranın prognozunun iyileştirilebileceği düşünüldü. Yüksek seviyede alkalen fosfotaz, kan üre azotu ve kreatinin konsantrasyonu ile birlikte 0.96 EU/mL'nin üstündeki kan endotoksin düzeyine sahip endotoksemik köpeklerde akut endometritis, akut metritis veya ...

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Köpeklerde Endotoksik Pyometranın Klinik, Bakteriyolojik ve Histopatolojik Özellikleri

Demırel¹,

Vural²,

Vural³

et al. 2018

Kafkas Univ Vet Fak Derg

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“…There were 44 eligible publications (Llazani et al n.d., de Schepper et al 1987, van Bree et al 1988, Fransson et al 1997, Faldyna et al 2001, Nak et al 2001, Fransson 2003, Fransson et al 2004, Hagman et al 2006a,b, Fransson et al 2007, Kilinç 2008, Hagman et al 2009, Küplülü et al 2009, Hagman 2011, Emanuelli et al 2012, Karlsson et al 2012, Radwińska et al 2012, Patil et al 2013, Sharif et al 2013, Ambrosen 2014, Enginler et al 2014, Jitpean et al 2014, Klainbart et al 2014, Voorwald et al 2015, Dąbrowski et al 2015a, 2015b, Gultiken et al 2016, Karabulut et al 2016, Karlsson et al 2016, Lidén 2017, Karabulut & Sonmez 2018, Tecles et al 2018, Alkan et al 2020, Gasser et al 2020, Hadiya et al 2020, Koenhemsi et al 2020, Maharathi et al 2020, Pugliese et al 2020, Ahn et al 2021, Günay Uçmak et al 2021…”

Section: Study Characteristics and Risk Of Bias Assessmentmentioning

confidence: 99%

The assessment of haematologic and serum chemistry parameters in canine pyometra: a systematic review and meta‐analysis

Demeli

Meyer

2023

J of Small Animal Practice

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ObjectivesPyometra is common in non‐spayed adult female dogs requiring early diagnosis and treatment to increase the survival rate. The diagnosis of pyometra is mainly based on clinical examination and anamnesis. Radiography, ultrasonography and laboratory analyses are recommended to support a diagnosis. The aim of this study was to assess blood parameters associated with pyometra by performing a systematic review and meta‐analysis.Materials and MethodsThe review was conducted in accordance with PRISMA guidelines. A search of three databases (PubMed, Google Scholar and CAB abstracts) was performed in July 2022. Studies providing information about laboratory parameters for both the pyometra group and healthy control group dogs were eligible for inclusion. Data extraction included the first author’s name, publication year, country, number of participants in both groups, mean values of selected studies, standard deviation values, and blood parameters. The risk of bias for each study was evaluated, and a random‐effects meta‐analysis was performed.ResultsWe included 44 studies which investigated 12 blood parameters. High heterogeneity was detected in all parameters in these meta‐analyses. The following blood parameters were increased in dogs with pyometra: white blood cell (mean=27.75×109 L−1, mean difference (MD)=17.16, 95% confidence interval (CI) 14.85 to 19.47), monocytes (mean=2.06×1012/L, MD=1.37, 95% CI 0.99 to 1.74), blood urea nitrogen (mean=41.42 mg/dL MD=18.06, 95% CI 12.26 to 23.85), alkaline phosphatase (mean=212.78 IU/L, MD=137.51, 95% CI 81.81 to 88.62), and aspartate aminotransferase (mean=48.31 IU/L, MD=16.96, 95% CI 10.61 to 23.30). The following parameters were reduced: red blood cell (mean=5.42 1012/L, MD=−1.37, 95% CI −1.68 to −1.05), haemoglobin (mean=121.20 g/L, MD=−30.57, 95% CI −39.70 to 21.45), albumin (mean=23.71 g/L, MD = −8.16, 95% CI −11.46 to −4.86). Lymphocyte, creatinine, urea, and alanine transaminase parameters were increased in some studies and decreased in others.Clinical SignificanceIn canine pyometra, some blood parameters are consistently increased, some consistently decreased, and some increased or decreased depending on the study.

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“…Elevated levels of blood urea nitrogen (BUN) and serum creatinine in the ovariohysterectomized bitch were linked to higher postoperative mortality rates (Kuplulu et al, 2009). Further, progression of pyometra into sepsis and elevated serum creatinine levels were linked to extended hospital stays, mortality, or both (Sant'Anna et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Several studies emphasize the importance of clinical evaluation of bitches with pyometra to make any decision for treatment and hospitalization, chances of post‐treatment complications and mortality (Hagman, 2012; Jitpean et al, 2014; McCallin et al, 2021). Elevated levels of blood urea nitrogen (BUN) and serum creatinine in the ovariohysterectomized bitch were linked to higher postoperative mortality rates (Kuplulu et al, 2009). Further, progression of pyometra into sepsis and elevated serum creatinine levels were linked to extended hospital stays, mortality, or both (Sant'Anna et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Secretory leucocyte protease inhibitor (SLPI) as an adjunct prognostic biomarker for canine pyometra

Sasidharan,

Patra,

Khan

et al. 2023

Reprod Domestic Animals

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Pyometra is a potentially life‐threatening condition that affects intact female dogs in their middle to advance age. Timely diagnosis and appropriate treatment are critical for the survival of patients, especially when pyometra advances to sepsis. This study aimed to investigate the prognostic potential of certain haematology, serum biochemical and inflammatory biomarker, secretory leucocyte protease inhibitor (SLPI) for pyometra in bitches (n = 41). Blood samples were collected after clinical diagnosis of pyometra for haematology and serum biochemistry. Based on the prognosis following medical/surgical treatment, animals were retrospectively categorized into survivor (n = 29) and dead (n = 12). Endometrial tissue sections were obtained from the bitches undergoing ovariohysterectomy (n = 21). Serum concentration of SLPI was quantified using sandwich ELISA and its expression in the endometrium was investigated using RT‐qPCR. A marked increase in the total leucocyte count (TLC), neutrophils, blood urea nitrogen (BUN) and serum creatinine was observed in the female dogs that did not survive. Significant elevation in the serum SLPI concentration (3.49 ± 0.44 vs. 2.38 ± 0.13 ng/mL) was observed in the bitches those died after the treatment, in comparison to those survived (p < .01). Additionally, there was a notable upregulation of SLPI in the endometrium in the bitches those died due to pyometra. Based on the ROC analysis results, it was observed that a cut‐off concentration of 2.93 ng/mL for SLPI, 27.77 mg/dL for BUN and 16.3 × 103/μL for TLC could effectively distinguish the prognosis of pyometra‐affected dogs. From this study, it can be concluded that upregulation of SLPI in the endometrium and its elevated concentration in peripheral circulation along with TLC and BUN concentration could serve as valuable indicators for predicting the prognosis of pyometra in bitches.

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The comparative evaluation of serum biochemical, haematological, bacteriological and clinical findings of dead and recovered bitches with pyometra in the postoperative process

Cited by 14 publications

References 15 publications

Köpeklerde Endotoksik Pyometranın Klinik, Bakteriyolojik ve Histopatolojik Özellikleri

Köpeklerde Endotoksik Pyometranın Klinik, Bakteriyolojik ve Histopatolojik Özellikleri

The assessment of haematologic and serum chemistry parameters in canine pyometra: a systematic review and meta‐analysis

Secretory leucocyte protease inhibitor (SLPI) as an adjunct prognostic biomarker for canine pyometra

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