Characterization of transforming growth factor beta superfamily, growth factors, transcriptional factors, and lipopolysaccharide in bovine cystic ovarian follicles

Polat, İbrahim; Alcigir, Eray; Pekcan, Mert; Vural, Seçil; Özenç, Erhan; Canatan, Halit; Küplülü, Şükrü; Dal, Gamze Evkuran; Yazlık, Murat Onur; Baklacı, C.; Vural, Mehmet Rıfat

doi:10.1016/j.theriogenology.2015.06.003

Cited by 13 publications

(8 citation statements)

References 37 publications

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“…However, it was reported that no correlation was found between plasma estradiol-17ß and progesterone concentrations and the number ovarian cysts ≤0.5 cm diameter; and that the endocrine activity of cysts located on the same ovary was independent of other cysts [11]. Also, formation of cystic ovarian follicles might be a result of inefficient LH stimulation, absence of LH receptors in their structures or the carryover effect of uterine endotoxins like in cattle [22]. The possibility of ovulation of follicles that are too small, but which contain LH receptors, could explain why animals with cystic ovaries exhibited physiologic cycle activity.…”

Section: Discussionmentioning

confidence: 99%

Effects of Ovarian Pathologies and Uterine Inflammations on Adenomyosis in Bitches

Yazlık

Tunc

Çolakoğlu

et al. 2018

Acta Scientiae. Vet.

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Background: Adenomyosis is a non-neoplastic condition, characterized by the presence of endometrial glands or cells in the myometrium. Adenomyosis is defined by post-operative microscopic examination of uterine tissue and a rarely seen infertility problem in bitches. The process of transformation of healthy uterus into adenomiotic uterus is still a mystery. We aimed to determine role of ovarian pathologies and uterine inflammations on pathogenesis of uterine adenomyosis in bitches.Cases: After ovariohysterectomy, the uteri were fixed and processed by routine methods. Sections were stained with HematoxylinEosin, Masson’s trichrome and Periodic-Acid-Schiff and immunohistochemically; with p53 protein, Ki-67, α-smooth-muscle actin, cytokeratin-CK 8, 14, 19 antibodies, estrogen and progesterone receptors by Avidin-Biotin-Complex-Peroxidase method. Histopathologically, 7 of the collected tissue samples (n = 38) were diagnosed as adenomyosis. There were unilateral (n = 6) or bilateral (n = 1) adenomyosis in which endometrial glands had broken through the myometrium, and in some cases there were fibromyoma, inflammation, papillary hyperplasia and cysts in glands. Vaginal fibroma, fibromyoma and benign mix tumor (n = 1), simple carcinoma (n = 3), carcinosarcoma (n = 3) in mammary gland were determinated histopathologically. In addition to these findings, follicular cysts (n = 4), parovarian cyst (n = 1), adenocarcinoma and Rete ovarii adenoma were diagnosed in some ovaries. The CK 19 was scored at endometrial glands (n = 2) positively and also, muscle fibers were stained positively with α-smooth-muscle actin antibody (n = 7).Discussion: Adenomyosis is an infertility problem in adult dogs and is hard to detect with routine gynecological examinations. Adenomyosis can be seen unaccompanied by other gynecological problems such as endometritis or leiomyomas, while mammary tumors with adenomyosis have been previously reported in women and in only one case of a bitch. Little is known of the interrelationships between gross pathological and endocrine features of ovarian cysts in bitches. Due to the local hormonal imbalance, most of these ovarian tumors and cystic structures might be accompanied with uterine-vaginal and mammary gland pathologies, cystic endometrial hyperplasia-pyometra, adenomyosis, vaginal fibroma and mammary tumors. The hyperestrogenic metabolic state may promote both the invagination process and the overall spreading of adenomyosis into the myometrium. Adenomyosis is often associated with polyps, anovulation, hyperplasia without cytological atypia, and uterine leiomyoma which are produced by hyperestrogenic states. Prolactin appears to induce adenomyosis in mice and hyperprolactinemia may cause myometrial degeneration/weakness and subsequent endometrial invasion in the presence of ovarian steroids in women. Premature decline in progesterone, a problem ensues with prolactin release which can promote hyperprolactinemia or endogenous prolactin release. In addition to hormonal problems, uterine infections, immune factors and their products like cytokines and inflammatory mediators, oxidative stress and free radical metabolism also might have roles in the occurrence of adenomyosis. In the present study, four of seven adenomyotic uterine tissues had inflammation and 3 of 7 cases had pre-inflammatory changes. Changes in uterine tissues may increase cell infiltration and proliferation as well as the activity of immune cells which may promote development of adenomyosis in bitches. It is therefore possible that a defect in the p53 tumor suppressor gene may play an important role in the de novo neoplastic transformation of adenomyosis. In conclusion, adenomyosis can be observed bilaterally or unilaterally in dogs. Tissue damage under the influence of inflammation or hormonal disturbances might have a role in the occurrence of uterine adenomyosis.

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Section: Discussionmentioning

confidence: 99%

Effects of Ovarian Pathologies and Uterine Inflammations on Adenomyosis in Bitches

Yazlık

Tunc

Çolakoğlu

et al. 2018

Acta Scientiae. Vet.

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“…Several studies have investigated the role of the follicular fluid microenvironment in oocyte quality and IVF outcomes [5]. Previous research reported that the addition of IGF-1 to in vitro culture medium prevented apoptosis [26]. Most studies have employed in vitro cell culture techniques, with exogenous supplementation of IGF [6,27].…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies documented that perifollicular perfusion influenced oocyte competence, embryo viability, and implantation potential [14][15][16][17][18][19][20]. Studies confirmed that embryos from fertilized oocytes obtained from wellvascularized follicles (WVFs) yielded higher pregnancy rates than oocytes obtained from poorly vascularized follicles [21,26]. Therefore, it is reasonable to presume that WVFs provide a suitable follicular fluid milieu and highquality oocytes, with increased implantation potential.…”

Section: Introductionmentioning

confidence: 90%

“…In granulosa cells, IGF-1, together with gonadotrophins, promotes hormone secretion and follicular growth and prevent apoptosis of mature follicles. Although several studies have investigated IGF-1 in follicular fluid, most of these studies have involved animal models or in vitro cell culture techniques in humans and exogenous rather than endogenous IGF-1 [26,27]. There is no consensus on the role of IGF-1 in human follicular fluid [27][28][29][30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

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Perifollicular blood flow and its relationship with endometrial vascularity, follicular fluid EG-VEGF, IGF-1, and inhibin-a levels and IVF outcomes

Vural

Döğer

et al. 2016

J Assist Reprod Genet

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“…Transforming growth factor b (TGF-b) superfamily comprises TGF-bs, activins, bone morphogenetic proteins (BMPs) and inhibins, [1][2][3] which regulate a variety of biologic functions including cell growth, development, differentiation, migration and carcinogenesis. 4,5 TGF-b1, as the prominent member, has been extensively studied for its vital functions in pathophysiology.…”

Section: Introductionmentioning

confidence: 99%

Binding and biologic characterization of recombinant human serum albumin-eTGFBR2 fusion protein expressed in CHO cells

et al. 2017

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Transforming growth factor-β1 (TGF-β1) signaling is involved in cell metabolism, growth, differentiation, carcinoma invasion and fibrosis development, which suggests TGF-β1 can be treated as a therapeutic target extensively. Because TGF-β1 receptor type α(TGFBR2) is the directed and essential mediator for TGF-β1 signals, the extracellular domain of TGFBR2 (eTGFBR2), binding partner for TGF-β1, has been produced in a series of expression systems to inhibit TGF-β1 signaling. However, eTGFBR2 is unstable with a short half-life predominantly because of enzymatic degradation and kidney clearance. In this study, a fusion protein consisting of human eTGFBR2 fused at the C-terminal of human serum albumin (HSA) was stably and highly expressed in Chinese Hamster Ovary (CHO) cells. The high and stable expression sub-clones with Ig kappa signal peptide were selected by Western blot analysis and used for suspension culture. After fed-batch culture over 8 d, the expression level of HSA-eTGFBR2 reached 180 mg/L. The fusion protein was then purified from culture medium using a 2-step chromatographic procedure that resulted in 39% recovery rate. The TGF-β1 binding assay revealed that HSA-eTGFBR2 could bind to TGF-β1 with the affinity constant (K of 1.42 × 10 M) as determined by the ForteBio Octet System. In addition, our data suggested that HSA-eTGFBR2 exhibited a TGF-β1 neutralizing activity and maintained a long-term activity more than eTGFBR2. It concluded that the overexpressing CHO cell line supplied sufficient recombinant human HSA-eTGFBR2 for further research and other applications.

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Characterization of transforming growth factor beta superfamily, growth factors, transcriptional factors, and lipopolysaccharide in bovine cystic ovarian follicles

Cited by 13 publications

References 37 publications

Effects of Ovarian Pathologies and Uterine Inflammations on Adenomyosis in Bitches

Effects of Ovarian Pathologies and Uterine Inflammations on Adenomyosis in Bitches

Perifollicular blood flow and its relationship with endometrial vascularity, follicular fluid EG-VEGF, IGF-1, and inhibin-a levels and IVF outcomes

Binding and biologic characterization of recombinant human serum albumin-eTGFBR2 fusion protein expressed in CHO cells

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