Plant fertilization is a major issue in the context of increasing population and food risk, higher cost of fertilizers, and low target efficiency of traditional mineral fertilization practices. Alternatively, application of microbial inoculants to the soil can enhance the uptake of nutrients by plants and increase the efficiency of mineral fertilizers and manures. Encapsulation methods involve covering and protecting the microorganisms. Encapsulation of bacterial cells has been challenged and used mainly in the agricultural industry using processes, such as spray drying, interfacial polymerization, or cross-linking. Here, we review techniques for microbial inoculants and their benefits for sustainable agriculture. Techniques include fluidized bed, extrusion, molecular inclusion, coacervation, liposomes, ionic or inverse gelation, and oil-entrapped emulsion. Major topics discussed are formulation of microbial inoculants, conventional inoculants, bioencapsulation materials, bioencapsulation techniques, and future trends. We found that (1) conventional inoculant does not provide adequate protection for microorganisms. (2) Bioencapsulation improves the protection and controlled release of bacteria. (3) Sodium alginate is one of the most used products for the bioencapsulation of microorganisms. (4) The bioencapsulation of microbial inoculants is performed with the incorporation of an active ingredient into a matrix followed by a mechanical operation, and finally stabilization by a chemical or physical-chemical process. (5) Spray-drying process works on a continuous basis, low operating cost, and high quality of capsules in good yield, although the high temperature used in the process is not very appropriate for encapsulating non-spore-forming bacteria. 6) Fluid-bed process is a promising encapsulation technique for large-scale production in agricultural industry. (7) Ionic gelation is currently the most adequate method found to encapsulate bacteria. (8) Some advantages and drawbacks are found for each technique; therefore, the selection of suitable bioencapsulation method will depend on bacteria strain, cost, processing conditions, and handling.

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A study was performed to investigate the efficiency of rhizobacteria on solubilization of rock phosphate and their assimilation by wheat plants in quartz sand potted experiments. Two phosphate solubilizing bacteria, P. fluorescens and Serratia sp. were encapsulated in sodium alginate and potato starch beads and selected to investigate the variation on pH values, the enzymatic activity of alkaline and acid phosphatase and phosphate solubilization in Pikovskaya liquid medium. A relation between pH diminution and P solubilization was found. P solubilization of 89 and 93 μg mL -1 was observed with immobilized phosphate solubilizing bacteria, which was significantly higher compared to autoclaved alginate-starch beads. Higher values around 64% in P uptake by wheat plants after 60 days of growth was observed with immobilized P. fluorescens+3.25 ppm of P. The results demonstrated that inoculation of the immobilized rhizobacteria is a promising option for inoculant carriers to increase P level in plants wheat and could be an innovative technique for application in agricultural industry.

The objective of this study was to evaluate the individual and synergic effects of the application of Biochar (B), Humic Substances (HS), and Gypsum (G) on the soil properties of a saline–sodic soil, and plant growth and seed quality (polyphenols, protein and yield) of quinoa. Treatments included (B) 22 t ha−1, (HS) 5 kg ha−1, and (G) 47.7 t ha−1. Two quinoa genotypes from Arid Zones (AZ-51 and AZ-103) were selected and established in eight treatments. The B + HS + G combined treatment resulted in increases in root biomass of 206% and 176% in AZ-51 and AZ-103, respectively. Furthermore, electrical conductivity (ECe), sodium adsorption ratio (SAR), and exchangeable sodium percentage (ESP) decreased significantly in all treated soils. When compared to the control, ESP decreased 11-fold in the G treatment, and 9–13-fold in the B + G; B + HS; and B + HS + G treatments. Similarly, soil microbial biomass increased 112% and 322% in the B + HS + G treatment in AZ-51 and AZ-103 genotypes, respectively. Therefore, it can be concluded that the application of combined amendments (B + HS + G) represents an alternative for reclaiming degraded soils, including saline–sodic soils.

Wogonin is a flavonoid found in different plants such as roots of Scutellaria baicalensis Georgi distributed mainly in Asia and Europe. Dried root extracts of S. baicalensis with high content of wogonin, popularly known as “Huang-Qin” or Chinese or baical skullcap, have been used for long time in traditional Chinese medicine. Several health benefits are attributed to wogonin and derivatives showing anti-inflammatory, antiviral, anticancer, and antioxidant effects and more recently antineurodegenerative properties. Preclinical pharmacological activities of wogonin against diverse types of cancer such as breast, colorectal, and human gastric cancer will be presented in this review. In addition, studies on oxidative stress and bioavailability of wogonin will be discussed together with antineurodegenerative potential with special focus on Alzheimer’s disease. Outcomes extracted from the last preclinical studies related to therapeutic applications of wogonin will be commented and updated in this review. The scientific evidence collected in this review aims to encourage transfer of the preclinical evidence of wogonin to new clinical studies.

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