Starch filler and osmoprotectants improve the survival of rhizobacteria in dried alginate beads

Schoebitz, Mauricio; Simonin, Hélène; Poncelet, Denis

doi:10.3109/02652048.2012.665090

Cited by 70 publications

(79 citation statements)

References 29 publications

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“…Thus, using only alginate for microbial cells immobilization, it would not be adequate to protect bacteria during immobilization process, drying and storage. However, applying starch an inexpensive material for cells immobilization and one of the most abundant natural biopolymers (Hickman, 1999), would improve cells survival on storage conditions (Schoebitz et al, 2012) since this filler material to serve as a carbon source to the bacteria. Additionally, microbial cells immobilization helps to increase the survival rate and slow cells release into the soil (Rekha et al, 2007).…”

Section: Resultsmentioning

confidence: 99%

“…Gelling of alginate-starch beads was completed after 30 min in contact with the calcium solution. The collected humid beads were stored in sealed plastic flasks at 4°C (Schoebitz et al, 2012).…”

Section: Immobilization Processmentioning

confidence: 99%

“…Cells immobilization in alginatestarch has been demonstrated to be more advantageous to improve cells survival and reducing the risk of decreased survival allowing long term storage of bacterial inoculum (Schoebitz et al, 2012;Cassidy et al, 1996). A major role of inoculant carrier is to provide more suitable microenvironment for the prolonged survival into the soil (Rekha et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies on probiotic and rhizobacteria have revealed cells adhesion to starch granules (Crittenden et al, 2001;Schoebitz et al, 2009b). It has been demonstrated anassociation between cells adhesion and its use as a carbon source substrate by cells (Crittenden et al, 2001).…”

mentioning

confidence: 99%

“…Increasing the growth plant effects, the beads can be forced to release the bacteria by mechanical crushing, while the intact beads can serve for progressive release (Rekha et al, 2007). Nevertheless, the use of starch increase the dry matter of the beads (Schoebitz et al, 2012) to give better mechanical resistance (Tal et al, 1999) and allow a high cells survival and establishment of the microbial inoculants on soil conditions.…”

mentioning

confidence: 99%

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Effect of immobilized phosphate solubilizing bacteria on wheat growth and phosphate uptake

Schoebitz

Ceballos

Ciamp

2013

J. Soil Sci. Plant Nutr.

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A study was performed to investigate the efficiency of rhizobacteria on solubilization of rock phosphate and their assimilation by wheat plants in quartz sand potted experiments. Two phosphate solubilizing bacteria, P. fluorescens and Serratia sp. were encapsulated in sodium alginate and potato starch beads and selected to investigate the variation on pH values, the enzymatic activity of alkaline and acid phosphatase and phosphate solubilization in Pikovskaya liquid medium. A relation between pH diminution and P solubilization was found. P solubilization of 89 and 93 μg mL -1 was observed with immobilized phosphate solubilizing bacteria, which was significantly higher compared to autoclaved alginate-starch beads. Higher values around 64% in P uptake by wheat plants after 60 days of growth was observed with immobilized P. fluorescens+3.25 ppm of P. The results demonstrated that inoculation of the immobilized rhizobacteria is a promising option for inoculant carriers to increase P level in plants wheat and could be an innovative technique for application in agricultural industry.

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Section: Resultsmentioning

confidence: 99%

Section: Immobilization Processmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Effect of immobilized phosphate solubilizing bacteria on wheat growth and phosphate uptake

Schoebitz

Ceballos

Ciamp

2013

J. Soil Sci. Plant Nutr.

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Biofertilizers: Past, Present, and Future

Sharma

Sharma²

2021

Biofertilizers

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Encapsulation of Rhodopseudomonas palustrisKTSSR54 using beads from alginate/starch blends

Rohman

Kaewtatip

Kantachote

et al. 2020

J of Applied Polymer Sci

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Alginate beads are a promising carrier for biofertilizer delivery, but still possess drawbacks of low mechanical strength and bead shrinkage that result in poor appearance and inadequate cell protection. Blending alginate with starch was proposed as a solution to these problems, and here alginate hydrogels were prepared using a 2% (w/v) alginate dispersion blended with varying contents of gelatinized starch (0–5% w/v). The interaction produced a viscosity synergism that increased the complexity of the matrix network in the alginate/starch blends, producing a more suitable matrix for cell entrapment. Hydrogen bonding between alginate and starch influenced the viscosity of the various solutions in a way that was consistent with the FTIR spectra. The starch content also helped beads retain their spherical shape after drying. The starch supported the entrapment of bacterial cells (plant growth‐promoting bacterium Rhodopseudomonas palustris KTSSR54 as biofertilizer) in the matrix, which reduced cell loss. The highest entrapment efficiency of 70.83% was obtained at 4% (w/v) starch, while the entrapment efficiency of control beads was 50.56%. Overall, the appropriate content of starch mixed with alginate is conducive to changes in the morphology of microcapsules and increases in the amount of biological encapsulation.

show abstract

Starch filler and osmoprotectants improve the survival of rhizobacteria in dried alginate beads

Cited by 70 publications

References 29 publications

Effect of immobilized phosphate solubilizing bacteria on wheat growth and phosphate uptake

Effect of immobilized phosphate solubilizing bacteria on wheat growth and phosphate uptake

Biofertilizers: Past, Present, and Future

Encapsulation of Rhodopseudomonas palustrisKTSSR54 using beads from alginate/starch blends

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