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O Manual de métodos de análise microbiológica de alimentos e água é um manual de laboratório ilustrado contendo os métodos recomendados por órgãos internacionais (APHA, FDA, USDA, AOAC, ISO) aceitos pela Agência Nacional de Vigilância Sanitária (ANVISA). Cada capítulo traz uma revisão profunda e atualizada sobre o(s) microrganismo(s) tratado(s), incluindo posição taxonômica, mudanças na nomenclatura, características morfológicas e bioquímicas e epidemiologia. Oferece também comparações esquemáticas entre os métodos disponíveis, destacando suas diferenças e similaridades. A apresentação didática do passo a passo dos métodos em figuras esquemáticas permite uma rápida apreensão dos procedimentos, facilitando sua execução no dia a dia dos laboratórios.

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Ochratoxin A (OA)-producing fungi were identified in coffee at different stages of maturation. The toxin was quantified in coffee during terrace drying and in coffee stored in barns. By direct plating, a high level of contamination (100%) was found in the coffee beans studied, with the genus Aspergillus representing 33.2%, of which Aspergillus ochraceus and Aspergillus niger represented 10.3 and 22.9%, respectively, of the strains isolated from the coffee beans. The capacity to produce ochratoxin was determined in 155 strains of A. ochraceus and A. niger using both the agar plug method and extraction with chloroform, giving positive results for 88.1% of the A. ochraceus strains and 11.5% of the A. niger strains. Analysis for OA in the terrace and barn coffee samples showed that, independent of cultivar, year harvested, or production region, all except one of the samples analyzed showed mycotoxin levels below the limit suggested by the European Common Market (8 microg/kg), thus indicating that the problem is restricted and due to severe faults in harvesting and storage practices.

Aspergillus species produce important mycotoxins, in particular aflatoxins, produced by A. flavus and related species, and ochratoxin A, produced by A. ochraceus and related species and also A. carbonarius and (less commonly) A. niger. In this review we briefly discuss the distribution of toxigenic Aspergillus species in nuts, coffee and cocoa beans, dried fruits, grapes, maize, rice and small grains. Future perspectives of distribution of Aspergillus species in foods is briefly discussed taking into account the impacts of climate change and the resilience of these mycotoxigenic species.

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