We have developed a method for isolating high quality total RNA from N. crassa mycelia that reliably yields large quantities. It is possible to extract more than 50 minipreps at once.

Resumo -O objetivo deste trabalho foi avaliar a influência de dois sistemas de cultivo orgânico sobre crescimento, fenologia, nutrição e produtividade de cultivares de cafeeiro, e sobre as características químicas do solo. Seis cultivares de café foram avaliadas em monocultura a pleno sol e em cultivo sombreado com banana e Erythrina verna, em delineamento de blocos ao acaso, com quatro repetições. O sombreamento reduziu a taxa de crescimento das cultivares nos primeiros 15 meses de cultivo. Depois de três anos reduziu, também, o diâmetro, o número de ramos produtivos e de nós dos cafeeiros; aumentou a área foliar e o tamanho dos grãos; reduziu o teor de K do solo, e aumentou os teores de N e de Mg nas folhas do café. Na primeira etapa da colheita (março/2004), a produtividade média das cultivares foi menor no sistema sombreado, o que evidencia que o sombreamento retarda a maturação dos grãos. Na segunda etapa (junho/2004), houve diferença entre cultivares, porém não entre os sistemas de cultivo. A produtividade média total (soma das duas etapas) foi igual nos dois sistemas de cultivo. As cultivares Tupi, Icatu e Obatã são as mais promissoras para o cultivo sombreado.Termos para indexação: Coffea arabica, Musa sp., Erythrina verna, produtividade, produção orgânica, sombreamento. Organic cultivation of coffee cultivars grown under full sun and under shadingAbstract -The objective of this work was to evaluate the influence of two organic cultivation systems on the growth, phenology, nutrition, and productivity of coffee cultivars, as well as on soil chemical characteristics. Six coffee cultivars were evaluated under both a monoculture system under full sun, and an intercropped system with banana and Erythrina verna plants for shading. The experimental design adopted was the randomized blocks with four replicates. The shading reduced the growth rate of cultivars only during the first 15 months of cultivation. After three years of cultivation, the shading reduced the plant diameter, the number of branches and the number of nodes; increased the leaf area and grain size; reduced soil K content and increased N and Mg contents in coffee plant leaves. In the first harvesting (March 2004), the average yield of the cultivars was smaller in the shading system showing that this treatment delayed grain maturity. In the second harvesting (June 2004), there were differences among cultivars, but not between cropping systems. Yield total average for the two harvestings was the same in the two systems. The cultivars Tupi, Icatu, and Obatã are more promising in shading system.

Foi conduzido um ensaio de campo em Seropédica, Estado do Rio de Janeiro, para avaliar o efeito da solarização do solo na população infestante de tiririca (Cyperus rotundus) e na produtividade de hortaliças submetidas a manejo orgânico. A solarização correspondeu à cobertura do solo com polietileno transparente (50 mim) por um período de 210 dias. Houve um aumento da temperatura média da camada superficial do solo (0-10 cm) da ordem de 23%, em relação ao das parcelas não-solarizadas. Após a retirada do plástico, cultivaram-se: cenoura (Daucus carota 'Brasília'), repolho (Brassica oleracea var. capitata 'Astrus'), beterraba (Beta vulgaris 'Tall Top Early Wonder') e vagem-anã (Phaseolus vulgaris 'Alessa'). Em termos gerais, a solarização reduziu em 59% a reinfestação pela tiririca. Nas parcelas solarizadas, houve significativo incremento das produtividades de cenoura (28%), vagem (32%), beterraba (37%) e repolho (34%). A fixação biológica do nitrogênio, a julgar pela nodulação da vagem, não foi afetada pela solarização, mas a população de nematóides do solo sofreu considerável redução.

It is known that nitrogen and carbon limitation regulate conidiation in shaken liquid cultures (Guignard et al. 1984 Can.

Sexual and asexual differentiation of Neurospora crassa are influenced by nitrogen availability and blue light. Nitrogen Limitation induces the production of protoperithecia on solid medium and conidia in liquid medium. Both developmental processes are stimulated by blue light. We have analysed changes in mRNA levels for a variety of light-inducible genes (aZ, bli and con) under conditions of nitrogen limitation. We show that the photoregulated genes aZ-1, aZ-2, bli-4, bli-7, con-5 and con-10 are also regulated by nitrogen limitation. These genes exhibited nitrogen regulation in the nonphotoresponsive mutant strains wc-1 and wc-2. Therefore, the wc-1 and we-2 gene products, although necessary for photoregulation of these d, bli and con genes, are not required for nitrogen regulation.

In view of the low N concentration in organic fertilizers, it is necessary to use high rates of such fertilizers to attend coffee crop requirements. Hence, N is the most limiting nutrient for organic coffee production. The objective of this work was to evaluate the influence of sunn hemp (Crotalaria juncea) organic fertilization on the growth and nutritional status of coffee cultivars, as well as to quantify plant biomass and N input derived from biological nitrogen fixation, and their effect on soil chemical characteristics. The experiment consisted of six coffee (Coffea arabica) cultivars intercropped with and without sunn hemp sown in November 2001 and pruned at mid-height 76 days later. At 175 days, the standing biomass of the legume was cut, measuring dry mass, total N, P, K, Ca, Mg, and 15 N natural abundance, resulting 16 t ha -1 of dry mass and the recycling of 444, 21, 241, 191, and 44 kg ha -1 of N, P, K, Ca, and Mg, respectively. Cultivars 'Obatã' and 'Catuaí Vermelho' presented the highest growth rates in terms of plant height, while cultivars 'Icatu' and 'Oeiras' presented the lowest rates. Biological nitrogen fixation associated to the legume introduced more than 200 kg ha -1 of N, which is a demonstration that N fertilization in organic cropping systems is a valuable alternative. Intercropping lead to a constant coffee leaf N content during the entire cropping cycle, contrary to what was observed in plots grown without sunn hemp. Key words: Coffea arabica, Crotalaria juncea, biological nitrogen fixation, nutrient input, green manure TAXA DE CRESCIMENTO E ESTADO NUTRICIONAL DO CAFEEIRO EM SISTEMA DE PRODUÇÃO ORGÂNICORESUMO: Devido a baixa concentração de N nos fertilizantes orgânicos, são necessárias doses elevadas dessas fontes para suprir as exigências do cafeeiro. Por esta razão, o N é o nutriente mais limitante na cafeicultura orgânica. O objetivo do trabalho foi avaliar, em sistema orgânico de produção, a influência da Crotalaria juncea no crescimento e estado nutricional de cultivares de café, bem como quantificar o aporte de biomassa vegetal e de N via fixação biológica, além do efeito sobre as características químicas do solo. O experimento foi constituído por seis cultivares de café (Coffea arabica), cultivadas com e sem Crotalaria juncea. A crotalária foi semeada em novembro de 2001, e aos 76 dias foi podada a meia altura. Aos 175 dias, foi cortada e quantificada a biomassa vegetal, os teores de N, P, K, Ca, Mg e a abundância natural de 15 N. O cultivo da crotalária proporcionou o aporte de 16 t ha -1 de matéria seca e a reciclagem de 444, 21, 241, 191 e 44 kg ha -1 de N, P, K, Ca e Mg, respectivamente. Os cultivares 'Obatã' e 'Catuaí Vermelho' apresentaram as maiores taxas de crescimento em altura, enquanto os cultivares 'Icatu' e 'Oeiras', as menores taxas. A fixação biológica de N proporcionou um aporte de N superior a 200 kg ha -1 de N, demonstrando ser uma alternativa para o produtor fertilizar os sistemas orgânicos com N. O cultivo da crotalária permitiu que o teor de N ac...

Although Coffea arabica species has its origin in the African understories, there is great resistance on the part of the Brazilian producers for growing this species under agroforestry systems as they fear that shading reduces production. This study aimed at evaluating some vegetative traits and the productivity of organically grown coffee (Coffea arabica L.) cultivars under shaded and unshaded systems. Twelve treatments consisting of two cultivation systems (shaded and unshaded) and six coffee cultivars were arranged in randomized blocks with four replicates, in a split-plot scheme. Shading was provided by banana (Musa sp.) and coral bean plants (Erythrina verna). Shading delayed fruit maturation. Late maturation cultivars, such as the Icatu and the Obatã, matured early in both cultivation systems, while medium and early maturation cultivars presented late maturation. Cultivation in the shaded system increased the leaf area and the number of lower branches, decreased the number of productive nodes per branch, and increased the distance between the nodes and the number of leaves present in the branches. Cultivation in the unshaded system presented greater number of plants with branch blight in relation to plants grown in the shade. The productivity of the cultivars was not different, at 30.0 processed bags per hectare in the shaded system, and 25.8 processed bags per hectare in the unshaded system. The most productive cultivars in the shaded system were the Tupi, the Obatã, and the Catuaí, while no differences between cultivars were obtained in the unshaded system.

Urinary tract infections (UTIs) are among the most common bacterial infections in humans. Although a number of bacteria can cause UTIs, most cases are due to infection by uropathogenic Escherichia coli (UPEC). UPEC are a genetically heterogeneous group that exhibit several virulence factors associated with colonization and persistence of bacteria in the urinary tract. Caenorhabditis elegans is a tiny, free‐living nematode found worldwide. Because many biological pathways are conserved in C. elegans and humans, the nematode has been increasingly used as a model organism to study virulence mechanisms of microbial infections and innate immunity. The virulence of UPEC strains, characterized for antimicrobial resistance, pathogenicity‐related genes associated with virulence and phylogenetic group belonging was evaluated by measuring the survival of C. elegans exposed to pure cultures of these strains. Our results showed that urinary strains can kill the nematode and that the clinical isolate ECP110 was able to efficiently colonize the gut and to inhibit the host oxidative response to infection. Our data support that C. elegans , a free‐living nematode found worldwide, could serve as an in vivo model to distinguish, among uropathogenic E. coli , different virulence behavior.