Nitrogen regulation of blue light-inducible genes in Neurospora crassa

Sokolovsky, V. Yu.; Lauter, Frank-Roman; Müller‐Röber, Bernd; Ricci, Marta dos Santos Freire; Schmidhauser, Thomas J.; Russo, Vincenzo

doi:10.1099/00221287-138-10-2045

Cited by 39 publications

(20 citation statements)

References 35 publications

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“…Incubation of this fungus under nitrogen starvation increased the levels of the carB and carRA orthologues, al-1 and al-2 (38). As in F. fujikuroi, this activation was independent of that produced by light, as indicated by the similar result obtained with wc mutants, totally devoid of photocarotenogenesis.…”

Section: Discussionsupporting

confidence: 68%

“…The stimulation of the pathway by light is responsible for the orange pigmentation acquired on the surface of cultures of this fungus and is paralleled by a rapid increase in the mRNA levels of the structural car genes (33,34,42). In contrast, the key regulatory signal for gibberellin production is the exhaustion of the nitrogen source, a condition attained in media with a low N/C ratio (13,38). This regulation is mediated by the nitrogen catabolite regulatory protein, AreA (32,46).…”

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confidence: 99%

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Regulation of Carotenogenesis and Secondary Metabolism by Nitrogen in Wild-Type Fusarium fujikuroi and Carotenoid-Overproducing Mutants

Rodríguez-Ortiz

Limón

Ávalos

2009

Appl Environ Microbiol

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The fungus Fusarium fujikuroi (Gibberella fujikuroi MP-C) produces metabolites of biotechnological interest, such as gibberellins, bikaverins, and carotenoids. Gibberellin and bikaverin productions are induced upon nitrogen exhaustion, while carotenoid accumulation is stimulated by light. We evaluated the effect of nitrogen availability on carotenogenesis in comparison with bikaverin and gibberellin production in the wild type and in carotenoid-overproducing mutants (carS). Nitrogen starvation increased carotenoid accumulation in all strains tested. In carS strains, gibberellin and bikaverin biosynthesis patterns differed from those of the wild type and paralleled the expression of key genes for both pathways, coding for geranylgeranyl pyrophosphate (GGPP) and kaurene synthases for the former and a polyketide synthase for the latter. These results suggest regulatory connections between carotenoid biosynthesis and nitrogen-controlled biosynthetic pathways in this fungus. Expression of gene ggs1, which encodes a second GGPP synthase, was also derepressed in the carS mutants, suggesting the participation of Ggs1 in carotenoid biosynthesis. The carS mutations did not affect genes for earlier steps of the terpenoid pathway, such as fppS or hmgR. Light induced carotenoid biosynthesis in the wild type and carRA and carB levels in the wild-type and carS strains irrespective of nitrogen availability.

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Section: Discussionsupporting

confidence: 68%

mentioning

confidence: 99%

Regulation of Carotenogenesis and Secondary Metabolism by Nitrogen in Wild-Type Fusarium fujikuroi and Carotenoid-Overproducing Mutants

Rodríguez-Ortiz

Limón

Ávalos

2009

Appl Environ Microbiol

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“…2). A similar effect has been observed in the transcriptional activation of the N. crassa genes con-5 and con-10 in response to nitrogen limitation in the wc-1 and wc-2 mutants (Sokolovsky et al, 1992). These results indicate the existence of a blue-light perception pathway independent of the BLR-1 and BLR-2 proteins, which exerts transcriptional regulation of gene expression in parallel to that controlled by the BLR complex.…”

Section: Identification Of Novel Blue-light-regulated Genes In T Atrsupporting

confidence: 76%

Novel light-regulated genes in Trichoderma atroviride: a dissection by cDNA microarrays

et al. 2006

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The influence of light on living organisms is critical, not only because of its importance as the main source of energy for the biosphere, but also due to its capacity to induce changes in the behaviour and morphology of nearly all forms of life. The common soil fungus Trichoderma atroviride responds to blue light in a synchronized manner, in time and space, by forming a ring of green conidia at what had been the colony perimeter at the time of exposure (photoconidiation). A putative complex formed by the BLR-1 and BLR-2 proteins in T. atroviride appears to play an essential role as a sensor and transcriptional regulator in photoconidiation. Expression analyses using microarrays containing 1438 unigenes were carried out in order to identify early light response genes. It was found that 2?8 % of the genes were light responsive: 2 % induced and 0?8 % repressed. Expression analysis in blr deletion mutants allowed the demonstration of the occurrence of two types of light responses, a blr-independent response in addition to the expected blr-dependent one, as well as a new role of the BLR proteins in repression of transcription. Exposure of T. atroviride to continuous light helped to establish that the light-responsive genes are subject to photoadaptation. Finally, evidence is provided of red-light-regulated gene expression and a possible crosstalk between the blue and red light signalling pathways.

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“…A major finding in the present study was that carbon deprivation did not induce conidiation in photoreceptor deletion mutants (blr mutants), indicating that the BLR proteins are involved in sensing different signals that allow the fungus to sporulate. In contrast, conidiation in response to nutrient deprivation of mutants in the orthologous genes in N. crassa was not altered (2,40). However, it has been shown in several other organisms that blue light generates changes in carbon metabolism.…”

Section: Discussionmentioning

confidence: 73%

Cross Talk between a Fungal Blue-Light Perception System and the Cyclic AMP Signaling Pathway

et al. 2006

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Blue light regulates many physiological and developmental processes in fungi. In Trichoderma atroviride the complex formed by the BLR-1 and BLR-2 proteins appears to play an essential role as a sensor and transcriptional regulator in photoconidiation. Here we demonstrate that the BLR proteins are necessary for carbon deprivation induced conidiation, even in the absence of light, pointing to the existence of an unprecedented cross talk between light and carbon sensing. Further, in contrast to what has been found in all other fungal systems, clear BLR-independent blue-light responses, including the activation of protein kinase A (PKA) and the regulation of gene expression, were found. Expression of an antisense version of the pkr-1 gene, encoding the regulatory subunit of PKA, resulted in a nonsporulating phenotype, whereas overexpression of the gene produced colonies that conidiate even in the dark. In addition, overexpression of pkr-1 blocked the induction of early light response genes. Thus, our data demonstrate that PKA plays an important role in the regulation of light responses in Trichoderma. Together, these observations suggest that the BLR complex plays a general role in sensing environmental cues that trigger conidiation and that such a role can be separated from its function as a transcription factor.

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Nitrogen regulation of blue light-inducible genes in Neurospora crassa

Cited by 39 publications

References 35 publications

Regulation of Carotenogenesis and Secondary Metabolism by Nitrogen in Wild-Type Fusarium fujikuroi and Carotenoid-Overproducing Mutants

Regulation of Carotenogenesis and Secondary Metabolism by Nitrogen in Wild-Type Fusarium fujikuroi and Carotenoid-Overproducing Mutants

Novel light-regulated genes in Trichoderma atroviride: a dissection by cDNA microarrays

Cross Talk between a Fungal Blue-Light Perception System and the Cyclic AMP Signaling Pathway

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